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Ecosystems - Genetics and Genomics

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Genetics and Genomics Capabilities

Methods and Equipment

Listed below are the genetics and genomics methods and equipment present at USGS facilities.

Facility
Location
Methods and Equipment

Alaska Science Center

 

Anchorage, Alaska
  • Cloning Techniques
  • DNA/Automated Sequencers
  • Electrophoresis Equipment
  • Multiplex PCR Thermocycler
  • Other:
    • Ancient DNA extraction
    • Collagen extraction for stable isotope analysis
    • DNA extraction from multiple sources
    • DNA quantification
    • Genetic imagery software
    • Marker development
    • SNP development
    • Virus sequencing

Arizona Fish and Wildlife Cooperative Research Unit

Tucson, Arizona

 

Columbia Environmental Research Center

Columbia, Missouri
  • Cloning Techniques
  • Cytometers
  • Electrophoresis Equipment
  • Mass Spectrometers
  • Real-time PCR Thermocycler
  • Other: Automated RNA isolation system

Florida Science Centers: Southeast Ecological Science Center and St. Petersburg Science Center

Florida
  • Cloning Techniques
  • DNA/Automated Sequencers
  • Electrophoresis Equipment
  • Multiplex PCR Thermocycler
  • Transcriptomics
  • DNA extraction from multiple sources
  • DNA quantification
  • Marker development

Forest and Rangeland Ecosystem Science Center

Corvallis, Oregon
  • Cloning Techniques
  • Electrophoresis Equipment
  • Multiplex PCR Thermocycler
  • Real-time PCR Thermocycler
  • Other:
    • Bio-imaging system
    • DNA purification from multiple sources
    • Genotyping
    • Marker development
  • Genetic Sampling Protocol (DOC, 55 KB) Word

Fort Collins Science Center: FORT Molecular Ecology Lab

Fort Collins, Colorado
  • Cloning Techniques
  • DNA/Automated Sequencers
  • Electrophoresis Equipment
  • Mass Spectrometers
  • Multiplex PCR Thermocycler
  • Real-time PCR Thermocycler
  • Other:
    • High-throughput robotics
    • Phosphoimager
    • Separate clean lab for ancient DNA work
    • Southern blot analysis
    • Transgenomics WAVE apparatus (denaturing high performance liquid chromatography)

Great Lakes Science Center

 

Ann Arbor, Michigan
  • DNA/Automated Sequencers
  • Electrophoresis Equipment
  • Multiplex PCR Thermocycler
  • Other:
    • Genotyping

Leetown Science Center

 

Kearneysville, West Virginia
  • Cloning Techniques
  • DNA/Automated Sequencers
  • Electrophoresis Equipment
  • Multiplex PCR Thermocycler
  • Real-time PCR Thermocycler
  • Other:
    • Software to facilitate analysis of eco-evolutionary dynamics using molecular markers
      • CREATE - assists with converting input data into various formats for use with over 50 genetic analysis programs
      • PEDAGOG - models eco-evolutionary dynamics
      • PedAgree - easily compares and assesses pedigree reconstruction using molecular markers

National Wetlands Research Center

 

Lafayette, Louisiana
  • DNA/Automated Sequencers
  • Multiplex PCR Thermocycler
  • Real-time PCR Thermocycler
  • Other:
    • Flow cytometry
    • Microplate reader
    • Microscopic image analysis

National Wildlife Health Center

 

Madison, Wisconsin
  • Cloning Techniques
  • Cytometers
  • DNA/Automated Sequencers
  • Electrophoresis Equipment
  • Mass Spectrometers
  • Multiplex PCR Thermocycler
  • Protein Sequencers
  • Real-time PCR Thermocycler

Northern Prairie Wildlife Research Center

Jamestown, North Dakota  

Northern Rocky Mountain Science Center

 

Bozeman, Montana  
Oregon Fish and Wildlife Cooperative Research Unit Corvallis, Oregon  

Pacific Islands Ecosystems Research Center

 

Honolulu, Hawaii  
Patuxent Wildlife Research Center Laurel, Maryland
  • Cloning Techniques
  • DNA/Automated Sequencers
  • Electrophoresis Equipment
  • Multiplex PCR Thermocycler
  • Real-time PCR Thermocycler

Texas Cooperative Fish and Wildlife Research Unit

Lubbock, Texas  

Upper Midwest Environmental Sciences Center

 

La Crosse, Wisconsin  

Western Ecological Research Center

 

Sacramento, California
  • Electrophoresis Equipment
  • Mass Spectrometers
  • Multiplex PCR Thermocycler
  • Other:
    • DNA extraction from multiple sources
    • DNA quantification
    • Gel imagery/documentation
    • Marker development, DNA quantification, DNA extraction

Western Fisheries Research Center

Seattle, Washington  
Wisconsin Cooperative Fishery Research Unit Stevens Point, Wisconsin  
Wisconsin Cooperative Wildlife Research Unit Madison, Wisconsin  
Voytek Microbiology Reston, Virginia  

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