Informing seed transfer guidelines and native plant materials development: Research supporting restoration across the Colorado Plateau and beyond Active
As restoration needs for natural landscapes grow due to higher frequency and/or intensity disturbances, pressure from invasive species, and impacts resulting from changing climates, considerable time and resources are being invested to guide the development and deployment of native plant materials (NPMs). Across lower elevations of the Colorado Plateau, a region composed primarily of public land where arid conditions make restoration especially challenging, NPM coordination has been spearheaded by the Bureau of Land Management’s Colorado Plateau Native Plant Program (CPNPP) since 2009.
To help CPNPP achieve its vision of healthy and resilient native plant communities, the Southwest Biological Science Center (SBSC) has provided scientific support and leadership since 2010. SBSC’s research includes field, lab, and greenhouse activities, many of which currently culminate in the development of species-specific seed transfer zones. These zones are developed to protect species’ natural patterns of genetic variation and depict species’ adaptations to regional climatic gradients so that managers and practitioners can make informed seed transfer and plant material decisions.
Background & Importance
The majority of native plant materials (NPMs) used for restoration represent wide-ranging species that provide important ecosystem services like stabilizing soils and filtering water.
Disturbed ecosystems benefit from the use of genetically appropriate NPMs or those that display ecological fitness, are compatible with other species in the plant community, and will not become invasive, because they can rejuvenate ecosystem function.
While many NPMs have been developed, a broader diversity of species and geographic sources is needed to provide the right choices in relation to a restoration site. Determining the right NPM for a restoration site can be tricky, which is why researchers develop tools like seed transfer zones.
Seed transfer zones are maps that allow managers and practitioners to easily match an NPM to areas where it may best be used in a restoration treatment. Guidance on seed transfer has been developed from common garden studies, molecular research, and climate modeling investigations.
All these approaches intend to improve the germination and establishment of NPMs at restoration sites, thereby supporting successful outcomes and stretching limited resources (e.g., time and money).
Genetic data (which hereafter refers to molecular/sequencing data) have broad utility when considering how to use existing NPMs or develop new NPMs. For example, landscape genetic analyses can illuminate patterns of genetic diversity across a species’ distribution and delineate the geographic distribution of evolutionary lineages.
Determining how long evolutionary lineages have been separated from one another and their rates of gene flow (e.g., cross-pollination) can help practitioners identify regions of a species’ range where individuals should not be mixed, even if those individuals occupy similar environments.
In addition, genetic diversity is well suited to determine taxonomic relationships when morphological variation is questionable. Landscape genetics can also identify adaptive genetic variation, or variation that may provide a benefit to the survival (and therefore reproductive capacity) of a species.
For most of the important Colorado Plateau restoration species, knowledge on adaptive differentiation, genetic diversity, and spatial variation in standing genetic diversity is unavailable.
General Methods
Leaf tissue samples for priority restoration species are field collected from sites located across the Colorado Plateau – sampling sites are stratified to represent the dominant, regional climatic gradients.
Colorado Plateau collections are often supplemented with leaf tissues from herbarium vouchers or collected by collaborators so as to incorporate/identify genetic patterns across species’ western distributions.
DNA is extracted and used to develop next-generation sequencing libraries at a laboratory at Northern Arizona University. Libraries are sequenced at the University of Oregon’s Genomics and Cell Characterization Core Facility on an Illumina HiSeq 6000. Data processing and analyses are completed at SBSC and take advantage of the USGS Yeti supercomputer.
Important Results
Genetically informed seed transfer zones for the Colorado Plateau and adjacent regions are available for eight species:
James’ galleta grass (Pleuraphis jamesii; syn. Hilaria jamesii); small-leaf globemallow (Sphaeralcea parvifolia); sand dropseed (Sporobolus cryptandrus); Rocky Mountain beeplant (Cleome serrulata); showy goldeneye (Heliomeris multiflora), rushy milkvetch (Astragalus lonchocarpus); yellow beeplant (Cleome lutea); and hoary tansyaster (Machaeranthera canescens).
Seed transfer zones reflect both genetic differentiation and putative adaptation and are available, along with the zones for other western species, from the Western Wildland Environmental Threat Assessment Center.
Future Directions
The Genetics for Western Restoration and Conservation (GWRC) research group has a variety of ongoing molecular and field-based projects.
For example, we are researching how the agricultural increase of seeds (i.e., the production of native plant materials) affects patterns of genetic diversity, as well as the long-term impacts of native plant materials on their conspecifics at restoration sites.
Please reach out to Rob Massatti (rmassatti@usgs.gov) for more information!
Below are other science projects associated with this project.
Plant Responses to Drought and Climate Change in the Southwestern United States
Aeolian Dust in Dryland Landscapes of the Western United States
RAMPS: Restoration Assessment & Monitoring Program for the Southwest
Big Sagebrush Ecosystem Response to Climate & Disturbance
Below are data or web applications associated with this project.
Below are publications associated with this project.
Assessment of population genetics and climatic variability can refine climate‐informed seed transfer guidelines
The historical context of contemporary climatic adaptation: A case study in the climatically dynamic and environmentally complex southwestern United States
Supporting the development and use of native plant materials for restoration on the Colorado Plateau (Fiscal Year 19 Report)
Genetically-informed seed transfer zones for Pleuraphis jamesii, Sphaeralcea parvifolia, and Sporobolus cryptandrus across the Colorado Plateau and adjacent regions
Earlier plant growth helps compensate for reduced carbon fixation after 13 years of warming
Supporting the development and use of native plant materials for restoration on the Colorado Plateau (Fiscal Year 18 Report)
Shrub persistence and increased grass mortality in response to drought in dryland systems
Population history provides foundational knowledge for utilizing and developing native plant restoration materials
Beyond traditional ecological restoration on the Colorado Plateau
Matching seed to site by climate similarity: techniques to prioritize plant materials development and use in restoration
Below are news stories associated with this project.
- Overview
As restoration needs for natural landscapes grow due to higher frequency and/or intensity disturbances, pressure from invasive species, and impacts resulting from changing climates, considerable time and resources are being invested to guide the development and deployment of native plant materials (NPMs). Across lower elevations of the Colorado Plateau, a region composed primarily of public land where arid conditions make restoration especially challenging, NPM coordination has been spearheaded by the Bureau of Land Management’s Colorado Plateau Native Plant Program (CPNPP) since 2009.
To help CPNPP achieve its vision of healthy and resilient native plant communities, the Southwest Biological Science Center (SBSC) has provided scientific support and leadership since 2010. SBSC’s research includes field, lab, and greenhouse activities, many of which currently culminate in the development of species-specific seed transfer zones. These zones are developed to protect species’ natural patterns of genetic variation and depict species’ adaptations to regional climatic gradients so that managers and practitioners can make informed seed transfer and plant material decisions.
Background & Importance
The majority of native plant materials (NPMs) used for restoration represent wide-ranging species that provide important ecosystem services like stabilizing soils and filtering water.
Disturbed ecosystems benefit from the use of genetically appropriate NPMs or those that display ecological fitness, are compatible with other species in the plant community, and will not become invasive, because they can rejuvenate ecosystem function.
While many NPMs have been developed, a broader diversity of species and geographic sources is needed to provide the right choices in relation to a restoration site. Determining the right NPM for a restoration site can be tricky, which is why researchers develop tools like seed transfer zones.
Seed transfer zones are maps that allow managers and practitioners to easily match an NPM to areas where it may best be used in a restoration treatment. Guidance on seed transfer has been developed from common garden studies, molecular research, and climate modeling investigations.
All these approaches intend to improve the germination and establishment of NPMs at restoration sites, thereby supporting successful outcomes and stretching limited resources (e.g., time and money).
Genetic data (which hereafter refers to molecular/sequencing data) have broad utility when considering how to use existing NPMs or develop new NPMs. For example, landscape genetic analyses can illuminate patterns of genetic diversity across a species’ distribution and delineate the geographic distribution of evolutionary lineages.
Determining how long evolutionary lineages have been separated from one another and their rates of gene flow (e.g., cross-pollination) can help practitioners identify regions of a species’ range where individuals should not be mixed, even if those individuals occupy similar environments.
In addition, genetic diversity is well suited to determine taxonomic relationships when morphological variation is questionable. Landscape genetics can also identify adaptive genetic variation, or variation that may provide a benefit to the survival (and therefore reproductive capacity) of a species.
For most of the important Colorado Plateau restoration species, knowledge on adaptive differentiation, genetic diversity, and spatial variation in standing genetic diversity is unavailable.
General Methods
Leaf tissue samples for priority restoration species are field collected from sites located across the Colorado Plateau – sampling sites are stratified to represent the dominant, regional climatic gradients.
Colorado Plateau collections are often supplemented with leaf tissues from herbarium vouchers or collected by collaborators so as to incorporate/identify genetic patterns across species’ western distributions.
DNA is extracted and used to develop next-generation sequencing libraries at a laboratory at Northern Arizona University. Libraries are sequenced at the University of Oregon’s Genomics and Cell Characterization Core Facility on an Illumina HiSeq 6000. Data processing and analyses are completed at SBSC and take advantage of the USGS Yeti supercomputer.
Important Results
Genetically informed seed transfer zones for the Colorado Plateau and adjacent regions are available for eight species:
James’ galleta grass (Pleuraphis jamesii; syn. Hilaria jamesii); small-leaf globemallow (Sphaeralcea parvifolia); sand dropseed (Sporobolus cryptandrus); Rocky Mountain beeplant (Cleome serrulata); showy goldeneye (Heliomeris multiflora), rushy milkvetch (Astragalus lonchocarpus); yellow beeplant (Cleome lutea); and hoary tansyaster (Machaeranthera canescens).
Seed transfer zones reflect both genetic differentiation and putative adaptation and are available, along with the zones for other western species, from the Western Wildland Environmental Threat Assessment Center.
Future Directions
The Genetics for Western Restoration and Conservation (GWRC) research group has a variety of ongoing molecular and field-based projects.
For example, we are researching how the agricultural increase of seeds (i.e., the production of native plant materials) affects patterns of genetic diversity, as well as the long-term impacts of native plant materials on their conspecifics at restoration sites.
Please reach out to Rob Massatti (rmassatti@usgs.gov) for more information!
- Science
Below are other science projects associated with this project.
Filter Total Items: 16Plant Responses to Drought and Climate Change in the Southwestern United States
Land managers face tremendous challenges in the future as drought and climate change alter the abundance, distribution, and interactions of plant species. These challenges will be especially daunting in the southwestern US, which is already experiencing elevated temperatures and prolonged droughts, resulting in reduced soil moisture in an already water-limited environment. These changes will...Aeolian Dust in Dryland Landscapes of the Western United States
Dust emission caused by wind erosion has received considerable attention because of its far-reaching effects on ecosystems, including the loss of nutrients and water-holding capacity from source areas, changes to climate and global energy balance in areas where dust is entrained in the atmosphere, fertilization of terrestrial and marine ecosystems, in addition to decreases in snow albedo, causing...RAMPS: Restoration Assessment & Monitoring Program for the Southwest
The Restoration Assessment and Monitoring Program for the Southwest (RAMPS) seeks to assist U.S. Department of the Interior (DOI) and other land management agencies in developing successful techniques for improving land condition in dryland ecosystems of the southwestern United States. Invasion by non-native species, wildfire, drought, and other disturbances are growing rapidly in extent and...Big Sagebrush Ecosystem Response to Climate & Disturbance
Big sagebrush ecosystems are a major component of landscapes in the western U.S. and provide vital habitat to a wide array of wildlife species. However, big sagebrush ecosystems have been dramatically impacted by disturbances in the past several decades. This collaborative research between USGS and the University of Wyoming focuses on understanding how climatic and soil conditions influence big... - Data
Below are data or web applications associated with this project.
- Publications
Below are publications associated with this project.
Filter Total Items: 22Assessment of population genetics and climatic variability can refine climate‐informed seed transfer guidelines
Restoration guidelines increasingly recognize the importance of genetic attributes in translocating native plant materials (NPMs). However, when species‐specific genetic information is unavailable, seed transfer guidelines use climate‐informed seed transfer zones (CSTZs) as an approximation. While CSTZs may improve how NPMs are developed and/or matched to restoration sites, they overlook genetic fAuthorsRobert Massatti, Robert K. Shriver, Daniel E. Winkler, Bryce A. Richardson, John B. BradfordThe historical context of contemporary climatic adaptation: A case study in the climatically dynamic and environmentally complex southwestern United States
The process of adaptation can be highly dependent upon historical and contemporary factors, especially in environmentally and topographically complex regions affected by Pleistocene glaciations. Here, we investigate Hilaria jamesii (Poaceae), a dryland C4 graminoid, to test how patterns of adaptive genetic variation are linked to its glacial and post‐glacial history. We show that the species persiAuthorsRobert Massatti, L. Lacey KnowlesSupporting the development and use of native plant materials for restoration on the Colorado Plateau (Fiscal Year 19 Report)
A primary focus of the Bureau of Land Management’s (BLM’s) Colorado Plateau Native Plant Program (CPNPP) is to identify and develop appropriate native plant materials (NPMs) for current and future restoration projects. Multiple efforts have characterized the myriad challenges inherent in providing appropriate seed resources to enable effective, widespread restoration and have identified a broad suAuthorsRobert Massatti, Daniel E. Winkler, Sasha C. Reed, Michael C. Duniway, Seth M. Munson, John B. BradfordGenetically-informed seed transfer zones for Pleuraphis jamesii, Sphaeralcea parvifolia, and Sporobolus cryptandrus across the Colorado Plateau and adjacent regions
(Massatti) Introduction: The majority of native plant materials (NPMs) utilized for restoration purposes are developed for widely distributed species that provide a variety of ecosystem services (Wood et al. 2015; Butterfield et al. 2017). Disturbed ecosystems benefit from the use of appropriate NPMs, which are those that display ecological fitness at the restoration site, are compatible with consAuthorsRobert MassattiEarlier plant growth helps compensate for reduced carbon fixation after 13 years of warming
1. Drylands play a dominant role in global carbon cycling and are particularly vulnerable to increasing temperatures, but our understanding of how dryland ecosystems will respond to climatic change remains notably poor. Considering that the area of drylands is projected to increase 11–23% by 2100, understanding the impacts of warming on the functions and services furnished by these arid and semiarAuthorsDaniel E. Winkler, Charlotte Grossiord, Jayne Belnap, Armin J. Howell, Scott Ferrenberg, Hilda J. Smith, Sasha C. ReedSupporting the development and use of native plant materials for restoration on the Colorado Plateau (Fiscal Year 18 Report)
Introduction A primary focus of the Colorado Plateau Native Plant Program (CPNPP) is to identify and develop appropriate native plant materials (NPMs) for current and future restoration projects. Multiple efforts have characterized the myriad challenges inherent in providing appropriate seed resources to enable effective, widespread restoration and identified a broad suite of research activities tAuthorsRobert Massatti, Daniel Winkler, Sasha Reed, Michael Duniway, Seth Munson, John BradfordShrub persistence and increased grass mortality in response to drought in dryland systems
Droughts in the southwest United States have led to major forest and grassland die‐off events in recent decades, suggesting plant community and ecosystem shifts are imminent as native perennial grass populations are replaced by shrub‐ and invasive plant‐dominated systems. These patterns are similar to those observed in arid and semiarid systems around the globe, but our ability to predict which spAuthorsDaniel E. Winkler, Jayne Belnap, David L. Hoover, Sasha C. Reed, Michael C. DuniwayPopulation history provides foundational knowledge for utilizing and developing native plant restoration materials
A species’ population structure and history are critical pieces of information that can help guide the use of available native plant materials in restoration treatments and decide what new native plant materials should be developed to meet future restoration needs. In the western United States, Pseudoroegneria spicata (bluebunch wheatgrass; Poaceae) is an important component of grassland and shrubAuthorsRobert Massatti, Holly R. Prendeville, Steve Larson, Bryce A. Richardson, Blair Waldron, Francis F. KilkennyBeyond traditional ecological restoration on the Colorado Plateau
The Colorado Plateau is one of North America's five major deserts, encompassing 340,000 km2 of the western U.S., and offering many opportunities for restoration relevant to researchers and land managers in drylands around the globe. The Colorado Plateau is comprised of vast tracts of public land managed by local, state, and federal agencies that oversee a wide range of activities (e.g., mineral anAuthorsDaniel E. Winkler, Dana M. Backer, Jayne Belnap, John B. Bradford, Bradley J. Butterfield, Stella M. Copeland, Michael C. Duniway, Akasha M. Faist, Stephen E. Fick, Scott L. Jensen, Andrea T. Kramer, Rebecca Mann, Robert Massatti, Molly L. McCormick, Seth M. Munson, Peggy Olwell, Steve D. Parr, Alix Pfennigwerth, Adrienne M. Pilmanis, Bryce A. Richardson, Ella Samuel, Kathy See, Kristina E. Young, Sasha C. ReedMatching seed to site by climate similarity: techniques to prioritize plant materials development and use in restoration
Land management agencies are increasing the use of native plant materials for vegetation treatments to restore ecosystem function and maintain natural ecological integrity. This shift toward the use of natives has highlighted a need to increase the diversity of materials available. A key problem is agreeing on how many, and which, new accessions should be developed. Here we describe new methods thAuthorsKyle Doherty, Bradley J. Butterfield, Troy E. Wood - Software
- News
Below are news stories associated with this project.
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