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Publications

Below is a list of available WFRC peer reviewed and published science.

Filter Total Items: 2475

Control of infectious Hematopoietic Necrosis virus disease by elevating the water temperature

Studies were performed to determine if increasing water temperatures could control infectious hematopoietic necrosis virus (IHN) disease in sockeye salmon (Oncorhynchus nerka). Mortalities could be prevented if the water temperature was raised to at least 18 C within the first 24 hr after infection of the fish and if the fish were maintained at this temperature for 4–6 days. The disease did not re
Authors
Donald F. Amend

Experimental control of Columnaris sisease with a new Nitrofuran drug, P-7138

P-7138 is a new, broad-spectrum, vinylogous, nitrofuran chemotherapeutic that was developed specifically for treating fish diseases. Although it can be added to the diet of fish, therapeutic tissue levels can be obtained in eels (Anguilla japonica) and goldfish (Carassius auratur) by absorption of the drug directly from the water. Moreover, the drug is eliminated from the tissues within hours and
Authors
Donald F. Amend, Avron J. Ross

Morphology of certain viruses of Salmonid Fishes. I. in vitro studies of some viruses causing Hematopoietic Necrosis

An electron microscope study was performed on three virus isolates that caused hematopoietic necrosis in salmonid fishes: infectious hematopoietic necrosis (IHN), Oregon Sockeye Disease (OSD), and Sacramento River Chinook Salmon Disease (SRCD). All three isolates were examined by negative staining of fathead minnow (FHM) monolayer tissue culture concentrates and IHN virus was also examined in thin
Authors
Donald F. Amend, Velma C. Chambers

Morphology of certain viruses of Salmonid fishes. II. In vivo studies of infectious Hematopoietic Necrosis Virus

Juvenile sockeye salmon (Oncorhynchus nerka) were injected with the infectious hematopoietic necrosis (IHN) virus, and tissue samples from the anterior kidney, spleen, liver, intestine, and pyloric caeca of moribund fish were prepared for electron microscopy. Bullet-shaped virus particles measuring 158 × 90 mμ were observed in the hematopoietic tissues of the anterior kidney and spleen. Virus part
Authors
Donald F. Amend, Velma C. Chambers

Precipitating antibody against Aeromonas salmonicida in serums of inbred albino Rainbow Trout (Salmo gairdneri)

Precipitins in albino rainbow trout serums were demonstrated by gel diffusion after a single parenteral exposure to the soluble antigens of Aeromonas salmonicida. The fraction of the serum containing antibody activity against the presented antigens was shown by immunoelectrophoresis to be in the nonmigrating region. This corresponded to the beta-2 fraction of rabbit serum. An antibody-containing c
Authors
Douglas P. Anderson, George W. Klontz

Stress of anesthesia with M.S. 222 and Benzocaine in Rainbow Trout (Salmo gairdneri)

Rainbow trout (Salmo gairdneri) anesthetized with M.S. 222 for periods up to 12 min experience interrenal ascorbate depletion, uremia, and moderate hypercholesterolemia. Anesthesia with neutralized M.S. 222 (pH 7) or benzocaine prevented these changes and significantly reduced the variability in plasma glucose, cholesterol, and cortisol, indicating that the stress of anesthesia with M.S. 222 is du
Authors
Gary Wedemeyer

Some blood chemistry values for the Rainbow Trout (Salmo gairdneri)

Normal distribution curves were graphically fitted to approximately 1400 clinical test values obtained from the plasma or kidney tissue of more than 200 yearling rainbow trout (Salmo gairdneri). Estimated normal ranges were ascorbate, 102–214 μg/g; blood urea nitrogen (BUN), 0.9–4.5 mg/100 ml; chloride, 84–132 mEq/liter; cholesterol, 161–365 mg/100 ml; cortisol, 1.5–18.5 μg/100 ml; glucose, 41–151
Authors
Gary Wedemeyer, K. Chatterton

An obscure disease of rainbow trout

An annul mortality among Rainbow Trout (Salmo gairdneri) has plagued the Shelton Hatchery of the Washington State Department of Game for the last several years. No infectious agent could be isolated from the moribund fish, but histopathologica1 changes in the liver of 1-month-old fish suggested the presence of a toxic substance. Scoliosis in 3-month-old fish suggested a possible deficiency in vita
Authors
R.R. Rucker, W. T. Yasutake, G. Wedemeyer

Occurrence of whirling disease of trout in western United States

Whirling disease of trout, caused by Myxosoma cerebralis, was diagnosed in 1966 for the first time in rainbow trout (Salmo gairdneri) from California and Nevada hatcheries. In 1969 the first incidence of this disease in cutthroat trout (S. clarki) was reported in Nevada. To date these have been the only confirmed cases of whirling disease in western United States.
Authors
H. Wolf, W. T. Yasutake

Stress-induced ascorbic acid depletion and cortisol production in two salmonid fishes

Interrenal ascorbic acid and serum cortisol were measured in non-specificity stressed yearling coho salmon and rainbow trout.Interrenal ascorbate was markedly decreased during stress but increased to normal if adaptation occurred.Serum cortisol was elevated by non-specific stress and remained high after interrenal ascorbate had returned to initial levels.
Authors
Gary Wedemeyer