Click here to download the Quality Assurance / Quality Control Manual
LINKS: Back to MIOH Microbiology Homepage
LIST OF APPENDICES
General Instructions
A1. Instructions for quality control of deionized water
A2. Buffer preparation
A3. Storage and maintenance of bacteria control cultures
A4. Laboratory map
Forms
B1. Temperature log sheet
B2. Sample log sheet
B3 Analytical Services Request Form
B4. Media and buffer quality-control log sheet
B5. Expendable supplies request form
B6. QC log for Actinomycetes detection by double agar layer method
B7. QC log for aerobic endospores by membrane filtration
Culture Methods
C1. M-Endo method for total coliforms
C2. MI agar method for total coliforms and Escherichia coli
C3. Colilert Quanti-Tray and presence/absence methods for total coliforms and Escherichia coli
C4. mFC method for fecal coliforms
C5. Modified mTEC method for Escherichia coli
C6. Colilert Quanti-Tray sediment method for Escherichia coli
C7. mEI method for enterococci
C8. Verification of enterococci colonies from mEI
C9. Enterolert Quanti-Tray for enterococci
C10. mCP agar method for Clostridium perfringens
C11. Indicator bacteria quality-control procedures
C12. Coliphage detection by USEPA Method 1602, single-agar layer method
C13. Coliphage detection by USEPA Method 1601, Two-step enrichment method
C14. Detection of Actinomycetes in water
C15. Detection of aerobic endospores in water
Molecular methods (appendices are available upon request)
D1. Collection and initial processing of water samples for MST markers and Cyanobacteria
D1a. Bench sheet for filtration and extraction for MST and Cyanobacteria
D2. Collection and initial processing of solid samples for MST markers and Cyanobacteria
D2a. Field sheet for collection of known-source fecal samples
D3. DNA extraction and purification using the GeneRite DNA-EZ kit
D3a. Extraction batch bench sheet
D4. Detection of MST markers and Cyanobacteria by qPCR
D4a. qPCR bench sheets for MST assays
D4b. qPCR bench sheets for Cyanobacteria DNA
D4c. RT-qPCR bench sheets for Cyanobacteria RNA
D5. Development and quantification of plasmid positive controls
D5a. Plasmid-based positive control bench sheet
D6. qPCR data interpretation and file management
D7. RNA extraction and purification using the MoBio PowerPlant RNA Isolation Kit with DNase
D8. Limits of blank, detection, and quantification for qPCR and qRT-PCR analyses
Enteric virus methods (appendices are available upon request)
E1. Virus elution and organic flocculation
E1a. Virus processing form
E1b. Virus qPCR batch sheet
E2. Molecular concentration and extraction
E2a. Foam elution and concentration from dead-end ultrafiltration
E3. Reverse transcription and qPCR protocol for viruses
E3a. qPCR and RT-qPCR benchsheets for viruses
E4. Total culturable virus assay
E4a. Total culturable virus and viral titering benchsheet
E5. Collection and initial processing of water samples for human polyomarvirus
E5a. Human polyomavirus filtration benchsheet
E6. Extraction for human polyomavirus samples
Cyanobacterial toxins
F1. Total microcystins - ADDA by ELISA
F1a. ELISA benchsheet and plate map template
- Overview
Click here to download the Quality Assurance / Quality Control Manual
LINKS: Back to MIOH Microbiology Homepage
LIST OF APPENDICES
General Instructions
A1. Instructions for quality control of deionized water
A2. Buffer preparation
A3. Storage and maintenance of bacteria control cultures
A4. Laboratory map
Forms
B1. Temperature log sheet
B2. Sample log sheet
B3 Analytical Services Request Form
B4. Media and buffer quality-control log sheet
B5. Expendable supplies request form
B6. QC log for Actinomycetes detection by double agar layer method
B7. QC log for aerobic endospores by membrane filtration
Culture Methods
C1. M-Endo method for total coliforms
C2. MI agar method for total coliforms and Escherichia coli
C3. Colilert Quanti-Tray and presence/absence methods for total coliforms and Escherichia coli
C4. mFC method for fecal coliforms
C5. Modified mTEC method for Escherichia coli
C6. Colilert Quanti-Tray sediment method for Escherichia coli
C7. mEI method for enterococci
C8. Verification of enterococci colonies from mEI
C9. Enterolert Quanti-Tray for enterococci
C10. mCP agar method for Clostridium perfringens
C11. Indicator bacteria quality-control procedures
C12. Coliphage detection by USEPA Method 1602, single-agar layer method
C13. Coliphage detection by USEPA Method 1601, Two-step enrichment method
C14. Detection of Actinomycetes in water
C15. Detection of aerobic endospores in water
Molecular methods (appendices are available upon request)
D1. Collection and initial processing of water samples for MST markers and Cyanobacteria
D1a. Bench sheet for filtration and extraction for MST and Cyanobacteria
D2. Collection and initial processing of solid samples for MST markers and Cyanobacteria
D2a. Field sheet for collection of known-source fecal samples
D3. DNA extraction and purification using the GeneRite DNA-EZ kit
D3a. Extraction batch bench sheet
D4. Detection of MST markers and Cyanobacteria by qPCR
D4a. qPCR bench sheets for MST assays
D4b. qPCR bench sheets for Cyanobacteria DNA
D4c. RT-qPCR bench sheets for Cyanobacteria RNA
D5. Development and quantification of plasmid positive controls
D5a. Plasmid-based positive control bench sheet
D6. qPCR data interpretation and file management
D7. RNA extraction and purification using the MoBio PowerPlant RNA Isolation Kit with DNase
D8. Limits of blank, detection, and quantification for qPCR and qRT-PCR analyses
Enteric virus methods (appendices are available upon request)
E1. Virus elution and organic flocculation
E1a. Virus processing form
E1b. Virus qPCR batch sheet
E2. Molecular concentration and extraction
E2a. Foam elution and concentration from dead-end ultrafiltration
E3. Reverse transcription and qPCR protocol for viruses
E3a. qPCR and RT-qPCR benchsheets for viruses
E4. Total culturable virus assay
E4a. Total culturable virus and viral titering benchsheet
E5. Collection and initial processing of water samples for human polyomarvirus
E5a. Human polyomavirus filtration benchsheet
E6. Extraction for human polyomavirus samples
Cyanobacterial toxins
F1. Total microcystins - ADDA by ELISA
F1a. ELISA benchsheet and plate map template