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Enhanced microbial coalbed methane generation: A review of research, commercial activity, and remaining challenges

Coalbed methane (CBM) makes up a significant portion of the world’s natural gas resources. The discovery that approximately 20% of natural gas is microbial in origin has led to interest in microbially enhanced CBM (MECoM), which involves stimulating microorganisms to produce additional CBM from existing production wells. This paper reviews current laboratory and field research on understanding pro
Authors
Daniel J. Ritter, David S. Vinson, Elliott P. Barnhart, Denise M. Akob, Matthew W. Fields, Al B. Cunningham, William H. Orem, Jennifer C. McIntosh

Large fractions of CO2-fixing microorganisms in pristine limestone aquifers appear to be involved in the oxidation of reduced sulfur and nitrogen compounds

The traditional view of the dependency of subsurface environments on surface-derived allochthonous carbon inputs is challenged by increasing evidence for the role of lithoautotrophy in aquifer carbon flow. We linked information on autotrophy (Calvin-Benson-Bassham cycle) with that from total microbial community analysis in groundwater at two superimposed—upper and lower—limestone groundwater reser
Authors
Martina Herrmann, Anna Rusznyák, Denise M. Akob, Isabel Schulze, Sebastian Opitz, Kai Uwe Totsche, Kirsten Küsel

Carbon flow from volcanic CO2 into soil microbial communities of a wetland mofette

Effects of extremely high carbon dioxide (CO2) concentrations on soil microbial communities and associated processes are largely unknown. We studied a wetland area affected by spots of subcrustal CO2 degassing (mofettes) with focus on anaerobic autotrophic methanogenesis and acetogenesis because the pore gas phase was largely hypoxic. Compared with a reference soil, the mofette was more acidic (Δp
Authors
Felix Beulig, Verena B. Heuer, Denise M. Akob, Bernhard Viehweger, Marcus Elvert, Martina Herrmann, Kai-Uwe Hinrichs, Kirsten Küsel

Identification of Mn(II)-oxidizing bacteria from a low-pH contaminated former uranium mine

Biological Mn oxidation is responsible for producing highly reactive and abundant Mn oxide phases in the environment that can mitigate metal contamination. However, little is known about Mn oxidation in low-pH environments, where metal contamination often is a problem as the result of mining activities. We isolated two Mn(II)-oxidizing bacteria (MOB) at pH 5.5 (Duganella isolate AB_14 and Albidife
Authors
Denise M. Akob, Tsing Bohu, Andrea Beyer, Franziska Schäffner, Matthias Händel, Carol A. Johnson, Dirk Merten, Georg Büchel, Kai Uwe Totsche, Kirsten Küsel

Surprising abundance of Gallionella-related iron oxidizers in creek sediments at pH 4.4 or at high heavy metal concentrations

We identified and quantified abundant iron-oxidizing bacteria (FeOB) at three iron-rich, metal-contaminated creek sites with increasing sediment pH from extremely acidic (R1, pH 2.7), to moderately acidic (R2, pH 4.4), to slightly acidic (R3, pH 6.3) in a former uranium-mining district. The geochemical parameters showed little variations over the 1.5 year study period. The highest metal concentrat
Authors
Maria Fabisch, Felix Beulig, Denise M. Akob, Kirsten Küsel