Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology
January 1, 2008
PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.
Citation Information
| Publication Year | 2008 |
|---|---|
| Title | Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology |
| DOI | 10.1128/AEM.01303-08 |
| Authors | Julia P. Galkiewicz, Christina A. Kellogg |
| Publication Type | Article |
| Publication Subtype | Journal Article |
| Series Title | Applied and Environmental Microbiology |
| Index ID | 70033136 |
| Record Source | USGS Publications Warehouse |
| USGS Organization | St. Petersburg Coastal and Marine Science Center |