Complex Mixtures, Complex Responses—Using Comprehensive Approaches to Assess Pharmaceutical Effects on Fish Completed
Fathead Minnows
Field Exposure Setup at a Wastewater Treatment Plant
The occurrence of complex mixtures of prescription pharmaceuticals in streams and rivers around the globe has raised concerns about potential unintended adverse effects on aquatic organisms. The deleterious effects (for example, alteration of fish behavior) observed in this study confirm that effluents containing pharmaceuticals can adversely affect fish in ways that are central to sustaining populations and that the effects may not be temporally consistent.
Prescription pharmaceuticals have been found in streams and lakes throughout the United States. They enter aquatic ecosystems primarily through treated municipal wastewater treatment plant (WWTP) effluent following human excretion or disposal of unwanted pharmaceuticals into the sewer system. Pharmaceuticals and their transformation products are generally present in low concentrations (less than 100 nanograms per liter) in most treated municipal wastewater effluents, but according to previous U.S. Geological Survey (USGS) research, they can be locally elevated in the effluent at WWTPs that receive incoming waste from pharmaceutical formulation facilities.
The present collaborative study between St. Cloud State University and USGS scientists used a comprehensive set of biological endpoints and minnow life stages in laboratory and field exposures to better understand the effects of pharmaceuticals on larval and adult fathead minnows (Pimephales promelas). This type of minnow was chosen because they are a common laboratory model for studies of this kind and are also an ecologically important species present throughout North America.
Larval and adult fathead minnows were exposed to treated municipal effluent from a WWTP known to receive input from a pharmaceutical formulation facility. In addition, both life stages were also exposed in the laboratory to the following nine pharmaceuticals individually or in mixtures: temazepam, a sleep aid; methocarbamol, a muscle relaxant; tramadol, an opioid agonist; hydrocodone, methadone, and oxycodone, opioids; and fluoxetine, paroxetine, and venlafaxine-antidepressants. The selected pharmaceuticals and corresponding exposure levels were guided by the previous USGS research.
The complexity of the effluent and laboratory exposures resulted in comparable complex biological responses. Juvenile fathead minnows exposed to the chemicals suffered from reduced growth and altered escape behavior. The altered escape behavior means that, when faced with a threat, the minnows did not escape as efficiently as they normally would, which could potentially increase the chances they would be eaten and could ultimately translate to population-level effects.
Adult fish reacted differently than juvenile fish to exposures. Adult females exposed to a mixture of the pharmaceuticals generally experienced an increase in relative liver size compared to control females, which suggests that the liver is reacting to pharmaceutical exposure. Adult males exposed to the pharmaceuticals had a variety of reactions. Most did not defend their nests as rigorously as those that were not exposed to the pharmaceuticals. The males exposed to WWTP effluent in the field component of this research produced a chemical known as plasma vitellogenin, a protein associated with egg production in females and not present in male fish under normal conditions.
This study documents that treated effluent containing pharmaceuticals and exposures to pharmaceuticals in a laboratory study can affect fish behavior and other biological endpoints. This study highlights the importance of including diverse biological endpoints spanning levels of biological organization and life stages when assessing the effects of pharmaceuticals. The environmental concentrations of prescription pharmaceuticals are usually below human or domestic animal therapeutic concentrations; however, these lower concentrations may still have effects on aquatic organisms that are exposed on a continual basis.
This study is part of a long-term effort by the USGS Toxic Substances Hydrology Program to determine the fate and effects of contaminants of emerging concern and to provide water-resource managers with objective information that assists in the development of effective water management practices. Understanding the impacts of environmental pharmaceutical occurrence in aquatic ecosystems may allow for effective strategies to reduce organism exposures.
This research was funded by the USGS Ecosystems Mission Area’s Environmental Health Program (Contaminant Biology and Toxic Substances Hydrology) and supported by the National Science Foundation (CBET 1336062).
Below are other science projects associated with this project.
Pharmaceutical fish: SCSU experiments: St. Cloud Times, December 2, 2015, news story (Hypertext links and other references to non-USGS products and services are provided for information only and do not constitute endorsement or warranty by the USGS, U.S. Department of the Interior, or U.S. Government)
Household Chemicals and Drugs Found in Biosolids from Wastewater Treatment Plants
National Reconnaissance of Pharmaceuticals, Hormones and Other Organic Wastewater Contaminants in U.S. Streams is Making an Impact
National Reconnaissance of Pharmaceuticals, Hormones, and Other Organic Wastewater Contaminants in Streams Named as One of the Top 100 Science Stories of the Year
Below are publications associated with this project.
Below are news stories associated with this project.
- Overview
The occurrence of complex mixtures of prescription pharmaceuticals in streams and rivers around the globe has raised concerns about potential unintended adverse effects on aquatic organisms. The deleterious effects (for example, alteration of fish behavior) observed in this study confirm that effluents containing pharmaceuticals can adversely affect fish in ways that are central to sustaining populations and that the effects may not be temporally consistent.
Prescription pharmaceuticals have been found in streams and lakes throughout the United States. They enter aquatic ecosystems primarily through treated municipal wastewater treatment plant (WWTP) effluent following human excretion or disposal of unwanted pharmaceuticals into the sewer system. Pharmaceuticals and their transformation products are generally present in low concentrations (less than 100 nanograms per liter) in most treated municipal wastewater effluents, but according to previous U.S. Geological Survey (USGS) research, they can be locally elevated in the effluent at WWTPs that receive incoming waste from pharmaceutical formulation facilities.
The present collaborative study between St. Cloud State University and USGS scientists used a comprehensive set of biological endpoints and minnow life stages in laboratory and field exposures to better understand the effects of pharmaceuticals on larval and adult fathead minnows (Pimephales promelas). This type of minnow was chosen because they are a common laboratory model for studies of this kind and are also an ecologically important species present throughout North America.
Larval and adult fathead minnows were exposed to treated municipal effluent from a WWTP known to receive input from a pharmaceutical formulation facility. In addition, both life stages were also exposed in the laboratory to the following nine pharmaceuticals individually or in mixtures: temazepam, a sleep aid; methocarbamol, a muscle relaxant; tramadol, an opioid agonist; hydrocodone, methadone, and oxycodone, opioids; and fluoxetine, paroxetine, and venlafaxine-antidepressants. The selected pharmaceuticals and corresponding exposure levels were guided by the previous USGS research.
The complexity of the effluent and laboratory exposures resulted in comparable complex biological responses. Juvenile fathead minnows exposed to the chemicals suffered from reduced growth and altered escape behavior. The altered escape behavior means that, when faced with a threat, the minnows did not escape as efficiently as they normally would, which could potentially increase the chances they would be eaten and could ultimately translate to population-level effects.
Adult fish reacted differently than juvenile fish to exposures. Adult females exposed to a mixture of the pharmaceuticals generally experienced an increase in relative liver size compared to control females, which suggests that the liver is reacting to pharmaceutical exposure. Adult males exposed to the pharmaceuticals had a variety of reactions. Most did not defend their nests as rigorously as those that were not exposed to the pharmaceuticals. The males exposed to WWTP effluent in the field component of this research produced a chemical known as plasma vitellogenin, a protein associated with egg production in females and not present in male fish under normal conditions.
This study documents that treated effluent containing pharmaceuticals and exposures to pharmaceuticals in a laboratory study can affect fish behavior and other biological endpoints. This study highlights the importance of including diverse biological endpoints spanning levels of biological organization and life stages when assessing the effects of pharmaceuticals. The environmental concentrations of prescription pharmaceuticals are usually below human or domestic animal therapeutic concentrations; however, these lower concentrations may still have effects on aquatic organisms that are exposed on a continual basis.
This study is part of a long-term effort by the USGS Toxic Substances Hydrology Program to determine the fate and effects of contaminants of emerging concern and to provide water-resource managers with objective information that assists in the development of effective water management practices. Understanding the impacts of environmental pharmaceutical occurrence in aquatic ecosystems may allow for effective strategies to reduce organism exposures.
This research was funded by the USGS Ecosystems Mission Area’s Environmental Health Program (Contaminant Biology and Toxic Substances Hydrology) and supported by the National Science Foundation (CBET 1336062).
- Science
Below are other science projects associated with this project.
Pharmaceutical fish: SCSU experiments: St. Cloud Times, December 2, 2015, news story (Hypertext links and other references to non-USGS products and services are provided for information only and do not constitute endorsement or warranty by the USGS, U.S. Department of the Interior, or U.S. Government)
Filter Total Items: 27Household Chemicals and Drugs Found in Biosolids from Wastewater Treatment Plants
Biosolids, the treated sludge generated by the treatment of sewage at wastewater treatment plants, is something that most people don't think about as they flush everyday chemicals and drugs down the drain. However, U.S. Geological Survey (USGS) scientists found that biosolids contain relatively high concentrations (hundreds of milligrams per kilogram) of the active ingredients commonly found in a...National Reconnaissance of Pharmaceuticals, Hormones and Other Organic Wastewater Contaminants in U.S. Streams is Making an Impact
The USGS's National Reconnaissance of Pharmaceuticals in U.S. Streams is making an impact in the scientific and regulatory communities.National Reconnaissance of Pharmaceuticals, Hormones, and Other Organic Wastewater Contaminants in Streams Named as One of the Top 100 Science Stories of the Year
Discover Magazine has named the Toxic Substances Hydrology Program's "National Reconnaissance of Pharmaceuticals, Hormones, and Other Organic Wastewater Contaminants in Streams" as one of the 100 top science stories of 2002. - Publications
Below are publications associated with this project.
- News
Below are news stories associated with this project.