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High-Content Screening Alternative Toxicity Testing— Columbia, Missouri Active

By Environmental Health Program August 30, 2018

Molecular Devices ImageXpress† High-Content Imaging System

72 hour post fertilization zebrafish

Zebrafish developmental cardiovascular toxicity high-content screen

72 hour post fertilization zebrafish under transmitted light

Imaging System can generate >25,000 images in less than 5 hours

About the Research

The Environmental Health Program works with toxicologists at the High-Content Screening Laboratory develop alternative toxicity testing to efficiently provide specific toxicity data to managers and regulators and prioritize compounds for further testing. Our high-content imaging capability provides a highly adaptable platform for early life stage fish toxicity testing at stages considered an animal alternative. Endpoints are directly relevant to adverse population-level effects.

Molecular Devices ImageXpress† High-Content Imaging System
Sources/Usage: Public Domain.
Molecular Devices ImageXpress† High-Content Imaging System(Credit: Jessica K. Leet, USGS. Public domain.)

Key Capabilities

  • Developmental cardiovascular toxicity assay
    • Pericardial edema/heart malformations, effects on angiogenesis, effects on heart rate and blood flow
    • Capable of screening pesticides, pharmaceuticals, mixtures, etc.
  • Establishment of additional endpoints/assays
    • The high-content imaging system is easily adjusted to add new endpoints to the current assay or develop new assays to meet needs for rapid toxicity testing and prioritizing of chemicals.
Using the 384-well plate format, a single zebrafish embryo is tested in each well
Sources/Usage: Public Domain.
Using the 384-well plate format, a single zebrafish embryo is tested in each well. This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under a fluorescein isothiocyanate (FITC) filter.(Credit: Jessica K. Leet, USGS. Public domain.)
The zebrafish developmental cardiovascular toxicity high-content screening assay uses a 384-well plate format
Sources/Usage: Public Domain.
The zebrafish developmental cardiovascular toxicity high-content screening assay uses a 384-well plate format to maximize number of treatments and replicates possible on each plate.(Credit: Jessica K. Leet, USGS. Public domain.)
This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under transmitted light
Sources/Usage: Public Domain.
This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under transmitted light. The same imaging system with was used.(Credit: Jessica K. Leet, USGS. Public domain.)

 

Key Instrumentation

High-content imaging system that allows for automated imaging of 384-well or 96-well plates, and semi-automated data extraction and analysis.

Molecular Devices ImageXpress† High-Content Imaging System
Sources/Usage: Public Domain.
Molecular Devices ImageXpress† High-Content Imaging System that can generate more than 25,000 images in less than 5 hours of automated image acquisition.(Credit: Jessica K. Leet, USGS. Public domain.)

 

 

 

  • Overview
    About the Research

    The Environmental Health Program works with toxicologists at the High-Content Screening Laboratory develop alternative toxicity testing to efficiently provide specific toxicity data to managers and regulators and prioritize compounds for further testing. Our high-content imaging capability provides a highly adaptable platform for early life stage fish toxicity testing at stages considered an animal alternative. Endpoints are directly relevant to adverse population-level effects.

    Molecular Devices ImageXpress† High-Content Imaging System
    Sources/Usage: Public Domain.
    Molecular Devices ImageXpress† High-Content Imaging System(Credit: Jessica K. Leet, USGS. Public domain.)

    Key Capabilities

    • Developmental cardiovascular toxicity assay
      • Pericardial edema/heart malformations, effects on angiogenesis, effects on heart rate and blood flow
      • Capable of screening pesticides, pharmaceuticals, mixtures, etc.
    • Establishment of additional endpoints/assays
      • The high-content imaging system is easily adjusted to add new endpoints to the current assay or develop new assays to meet needs for rapid toxicity testing and prioritizing of chemicals.
    Using the 384-well plate format, a single zebrafish embryo is tested in each well
    Sources/Usage: Public Domain.
    Using the 384-well plate format, a single zebrafish embryo is tested in each well. This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under a fluorescein isothiocyanate (FITC) filter.(Credit: Jessica K. Leet, USGS. Public domain.)
    The zebrafish developmental cardiovascular toxicity high-content screening assay uses a 384-well plate format
    Sources/Usage: Public Domain.
    The zebrafish developmental cardiovascular toxicity high-content screening assay uses a 384-well plate format to maximize number of treatments and replicates possible on each plate.(Credit: Jessica K. Leet, USGS. Public domain.)
    This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under transmitted light
    Sources/Usage: Public Domain.
    This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under transmitted light. The same imaging system with was used.(Credit: Jessica K. Leet, USGS. Public domain.)

     

    Key Instrumentation

    High-content imaging system that allows for automated imaging of 384-well or 96-well plates, and semi-automated data extraction and analysis.

    Molecular Devices ImageXpress† High-Content Imaging System
    Sources/Usage: Public Domain.
    Molecular Devices ImageXpress† High-Content Imaging System that can generate more than 25,000 images in less than 5 hours of automated image acquisition.(Credit: Jessica K. Leet, USGS. Public domain.)