Environmental DNA (eDNA): A tool for quantifying the abundant but elusive round goby (Neogobius melanostomus)
Environmental DNA (eDNA) is revolutionizing biodiversity monitoring, occupancy estimates, and real-time detections of invasive species. In the Great Lakes, the round goby (Neogobius melanostomus), an invasive benthic fish from the Black Sea, has spread to encompass all five lakes and many tributaries, outcompeting or consuming native species; however, estimates of round goby abundance are confounded by behavior and habitat preference, which impact reliable methods for estimating their population. By integrating eDNA into round goby monitoring, improved estimates of biomass may be obtainable. We conducted mesocosm experiments to estimate rates of goby DNA shedding and decay. Further, we compared eDNA with several methods of traditional field sampling to compare its use as an alternative/complementary monitoring method. Environmental DNA decay was comparable to other fish species, and first-order decay was lower at 12°C (k = 0.043) than at 19°C (k = 0.058). Round goby eDNA was routinely detected in known invaded sites of Lake Michigan and its tributaries (range log10 4.8–6.2 CN/L), but not upstream of an artificial fish barrier. Traditional techniques (mark-recapture, seining, trapping) in Lakes Michigan and Huron resulted in fewer, more variable detections than eDNA, but trapping and eDNA were correlated (Pearson R = 0.87). Additional field testing will help correlate round goby abundance with eDNA, providing insight on its role as a prey fish and its impact on food webs.
Citation Information
| Publication Year | 2018 |
|---|---|
| Title | Environmental DNA (eDNA): A tool for quantifying the abundant but elusive round goby (Neogobius melanostomus) |
| DOI | 10.1371/journal.pone.0191720 |
| Authors | Meredith B. Nevers, Muruleedhara Byappanahalli, Charles C. Morris, Dawn Shively, Katarzyna Przybyla-Kelly, Ashley M. Spoljaric, Joshua Dickey, Edward F. Roseman |
| Publication Type | Article |
| Publication Subtype | Journal Article |
| Series Title | PLoS ONE |
| Index ID | 70194981 |
| Record Source | USGS Publications Warehouse |
| USGS Organization | Great Lakes Science Center |
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Round goby eDNA survey, evaluation, and laboratory data in Lakes Michigan and Huron 2016-2017
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Bureau Approving Official, Biologist
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Research Microbiologist
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Round goby eDNA survey, evaluation, and laboratory data in Lakes Michigan and Huron 2016-2017
Data included are from a series of field sample collections from Lakes Michigan and Huron, and laboratory mesocosms targeting the round goby fish (Neogobius melanostomus). The round goby is a benthic fish that has heavily invaded four of the five Laurentian Great Lakes. Because it inhabits a variety of substrates, including coastal breakwaters, traditional methods (e.g., trawling, trapping) are in - Connect
Meredith B. Nevers
Bureau Approving Official, BiologistEmailPhoneMuruleedhara (Murulee) N Byappanahalli, PhD
Research MicrobiologistEmailPhoneExt421