Hepatic microsomal cytochromes P450 in mink fed Saginaw Bay carp (SBC)
January 1, 1992
Livers from mink fed diets containing 0% (n = 12), 10% (n = 11), 20% (n = 12) and 40% (n = 10) SBC for 6 months contained 0.1, 2.2, 3.6, and 6.3 ug/g total PCBs, respectively. Hepatic microsomes were prepared and assayed for protein, arylhydrocarbon hydroxylase (AHH), benzyloxyresorufin-O-dealkylase (BROD), ethoxy-ROD (ER0D), pentoxy-ROD (PROD), and ethoxycoumarin-OD (ECOD). Mink fed SBC had increased AHH, EROD, and ECOD (group means 2.2-3.4 X control means), decreased BROD and unchanged PROD (the latter 2 assays indicators for phenobarbital-type induction in mammals). Three samples from each group were examined by western blot using a polyclonal anti-P450llB antibody and a monoclonal anti-P450lA antibody (MAb 1-12-3). Mink fed SBC showed induction of a protein recognized by anti-P450lA (8 X control), but had little protein recognized by anti-P450IlB. The monooxygenase activities and western blot data give a consistent picture of MC-type but not PB-type induction in mink fed SBC.
Citation Information
| Publication Year | 1992 |
|---|---|
| Title | Hepatic microsomal cytochromes P450 in mink fed Saginaw Bay carp (SBC) |
| Authors | M. J. Melancon, L. LeCaptain, Barnett A. Rattner, S. Heaton, R. Aulerich, D. Tillitt, John J. Stegeman, B. Woodin |
| Publication Type | Article |
| Publication Subtype | Journal Article |
| Series Title | Society of Environmental Toxicology and Chemistry, annual meeting abstract book |
| Index ID | 5224016 |
| Record Source | USGS Publications Warehouse |
| USGS Organization | Patuxent Wildlife Research Center |