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Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting

January 1, 2011

Real-time, or quantitative, polymerase chain reaction (qPCR) is quickly supplanting other molecular methods for detecting the nucleic acids of human and other animal pathogens owing to the speed and robustness of the technology. As the aquatic animal health community moves toward implementing national diagnostic testing schemes, it will need to evaluate how qPCR technology should be employed. This review outlines the basic principles of qPCR technology, considerations for assay development, standards and controls, assay performance, diagnostic validation, implementation in the diagnostic laboratory, and quality assurance and control measures. These factors are fundamental for ensuring the validity of qPCR assay results obtained in the diagnostic laboratory setting.

Citation Information

Publication Year 2011
Title Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting
DOI 10.1080/08997659.2011.620217
Authors Maureen K. Purcell, Rodman G. Getchell, Carol A. McClure, S.E. Weber, Kyle A. Garver
Publication Type Article
Publication Subtype Journal Article
Series Title Journal of Aquatic Animal Health
Series Number
Index ID 70043655
Record Source USGS Publications Warehouse
USGS Organization Western Fisheries Research Center