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RAD sequencing yields a high success rate for westslope cutthroat and rainbow trout species-diagnostic SNP assays

July 1, 2012

Hybridization with introduced rainbow trout threatens most native westslope cutthroat trout populations. Understanding the genetic effects of hybridization and introgression requires a large set of high-throughput, diagnostic genetic markers to inform conservation and management. Recently, we identified several thousand candidate single-nucleotide polymorphism (SNP) markers based on RAD sequencing of 11 westslope cutthroat trout and 13 rainbow trout individuals. Here, we used flanking sequence for 56 of these candidate SNP markers to design high-throughput genotyping assays. We validated the assays on a total of 92 individuals from 22 populations and seven hatchery strains. Forty-six assays (82%) amplified consistently and allowed easy identification of westslope cutthroat and rainbow trout alleles as well as heterozygote controls. The 46 SNPs will provide high power for early detection of population admixture and improved identification of hybrid and nonhybridized individuals. This technique shows promise as a very low-cost, reliable and relatively rapid method for developing and testing SNP markers for nonmodel organisms with limited genomic resources.

Publication Year 2012
Title RAD sequencing yields a high success rate for westslope cutthroat and rainbow trout species-diagnostic SNP assays
DOI 10.1111/j.1755-0998.2012.03157.x
Authors Stephen J. Amish, Paul A. Hohenlohe, Sally Painter, Robb F. Leary, Clint C. Muhlfeld, Fred W. Allendorf, Gordon Luikart
Publication Type Article
Publication Subtype Journal Article
Series Title Molecular Ecology Resources
Index ID 70155347
Record Source USGS Publications Warehouse
USGS Organization Northern Rocky Mountain Science Center