David S Blehert
David Blehert is Chief of the National Wildlife Health Center's Laboratory Sciences Branch
David Blehert received a Ph.D. in Bacteriology from the University of Wisconsin-Madison in 1999, and he joined the USGS National Wildlife Health Center (NWHC) as a Diagnostic and Research Microbiologist in 2003. His research focuses on investigation of the ecology and pathobiology of bat white-nose syndrome. Today, Dr. Blehert is Chief of the NWHC's Laboratory Sciences Branch, which specializes in the investigation of unusual mortality events impacting our nation's wildlife. His laboratories also conduct surveillance for animal diseases of high consequence, such as white-nose syndrome in bats and highly pathogenic avian influenza viruses in wild waterfowl.
Professional Experience
2014 to present, Chief, Laboratory Sciences Branch, U.S. Geological Survey National Wildlife Health Center, Madison, Wisconsin
2003-2014, Diagnostic and Research Microbiologist, U.S. Geological Survey National Wildlife Health Center, Madison, Wisconsin
1999 to 2003, Intramural Research Training Associate Fellow, National Institutes of Health, Bethesda, Maryland
Education and Certifications
Ph.D. Bacteriology, University of Wisconsin-Madison, 1999
B.S. Biology, University of Minnesota-Minneapolis St. Paul, 1993
Affiliations and Memberships*
Fellow, American Academy of Microbiology. Elected in February, 2020.
Fellow, Canadian Institute for Advanced Research. CIFAR Program Fungal Kingdom: Threats and Opportunities. Elected in June, 2019.
Honorary Fellow, University of Wisconsin-Madison School of Veterinary Medicine, Department of Pathobiological Sciences. 2003-2018.
Member, Wildlife Disease Association. 2006-present.
Member, American Society for Microbiology. 1995-present.
Science and Products
Geomyces and Pseudogymnoascus: Emergence of a primary pathogen, the causative agent of bat white-nose syndrome
Snake fungal disease: An emerging threat to wild snakes
Detection of spring viraemia of carp virus in imported amphibians reveals an unanticipated foreign animal disease threat
A pelagic outbreak of avian cholera in North American gulls: Scavenging as a primary mechanism for transmission?
First detection of bat white-nose syndrome in western North America
Use of multiple sequencing technologies to produce a high-quality genome of the fungus Pseudogymnoascus destructans, the causative agent of bat White-Nose syndrome
Optimized methods for total nucleic acid extraction and quantification of the bat white-nose syndrome fungus, Pseudogymnoascus destructans, from swab and environmental samples
Experimental infection of snakes with Ophidiomyces ophiodiicola causes pathological changes that typify snake fungal disease
TaqMan real-time polymerase chain reaction for detection of Ophidiomyces ophiodiicola, the fungus associated with snake fungal disease
Avian botulism type E in waterbirds of Lake Michigan, 2010–2013
Direct detection of fungal siderophores on bats with white-nose syndrome via fluorescence microscopy-guided ambient ionization mass spectrometry
The fungus Trichophyton redellii sp. nov. causes skin infections that resemble white-nose syndrome of hibernating bats
Non-USGS Publications**
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
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Filter Total Items: 71
Geomyces and Pseudogymnoascus: Emergence of a primary pathogen, the causative agent of bat white-nose syndrome
Geomyces and Pseudogymnoascus (Fungi, Ascomycota, Leotiomycetes, aff. Thelebolales) are closely related groups of globally occurring soil-associated fungi. Recently, these genera of fungi have received attention because a newly identified species, Pseudogymnoascus (initially classified as Geomyces) destructans, was discovered in association with significant and unusual mortality of hibernating batAuthorsMichelle L. Verant, Andrew M. Minnis, Daniel L. Lindner, David S. BlehertSnake fungal disease: An emerging threat to wild snakes
Since 2006, there has been a marked increase in the number of reports of severe and often fatal fungal skin infections in wild snakes in the eastern USA. The emerging condition, referred to as snake fungal disease (SFD), was initially documented in rattlesnakes, where the infections were believed to pose a risk to the viability of affected populations. The disease is caused byOphidiomyces ophiodiiAuthorsJeffrey M. Lorch, Susan Knowles, Julia S. Lankton, Kathy Michell, Jaime L. Edwards, Joshua M. Kapfer, Richard A. Staffen, Erik R. Wild, Katie Z. Schmidt, Anne Ballmann, Doug Blodgett, Terence M. Farrell, Brad M. Glorioso, Lisa A. Last, Steven J. Price, Krysten L. Schuler, Christopher Smith, James F. X. Wellehan, David S. BlehertDetection of spring viraemia of carp virus in imported amphibians reveals an unanticipated foreign animal disease threat
Global translocation of plants and animals is a well-recognized mechanism for introduction of pathogens into new regions. To mitigate this risk, various tools such as preshipment health certificates, quarantines, screening for specific disease agents and outright bans have been implemented. However, such measures only target known infectious agents and their hosts and may fail to prevent translocaAuthorsHon S. Ip, Jeffrey M. Lorch, David S. BlehertA pelagic outbreak of avian cholera in North American gulls: Scavenging as a primary mechanism for transmission?
Avian cholera, caused by the bacterium Pasteurella multocida, is an endemic disease globally, often causing annual epizootics in North American wild bird populations with thousands of mortalities. From December 2006 to March 2007, an avian cholera outbreak caused mortality in marine birds off the coast of Atlantic Canada, largely centered 300–400 km off the coast of the island of Newfoundland. ScaAuthorsMichelle Wille, Scott McBurney, Gregory J. Robertson, Sabine Wilhelm, David S. Blehert, Catherine Soos, Ron Dunphy, Hugh WhitneyFirst detection of bat white-nose syndrome in western North America
White-nose syndrome (WNS) is an emerging fungal disease of bats caused by Pseudogymnoascus destructans. Since it was first detected near Albany, NY, in 2006, the fungus has spread across eastern North America, killing unprecedented numbers of hibernating bats. The devastating impacts of WNS on Nearctic bat species are attributed to the likely introduction of P. destructans from Eurasia to naive hoAuthorsJeffrey M. Lorch, Jonathan M. Palmer, Daniel L. Lindner, Anne Ballmann, Kyle George, Kathryn M. Griffin, Susan Knowles, John R. Huckabee, Katherine H. Haman, Christopher D. Anderson, Penny A. Becker, Joseph B. Buchanan, Jeffrey T. Foster, David S. BlehertUse of multiple sequencing technologies to produce a high-quality genome of the fungus Pseudogymnoascus destructans, the causative agent of bat White-Nose syndrome
White-Nose syndrome has recently emerged as one of the most devastating wildlife diseases recorded, causing widespread mortality in numerous bat species throughout eastern North America. Here, we present an improvised reference genome of the fungal pathogen Pseudogymnoascus destructans for use in comparative genomic studies.AuthorsKevin P. Drees, Jonathan M. Palmer, Robert Sebra, Jeffrey M. Lorch, Cynthia Chen, ChengCang Wu, Jin Woo Bok, Nancy F. Keller, David S. Blehert, Christina A. Cuomo, Daniel L. Linder, Jeffrey T. FosterOptimized methods for total nucleic acid extraction and quantification of the bat white-nose syndrome fungus, Pseudogymnoascus destructans, from swab and environmental samples
The continued spread of white-nose syndrome and its impacts on hibernating bat populations across North America has prompted nationwide surveillance efforts and the need for high-throughput, noninvasive diagnostic tools. Quantitative real-time polymerase chain reaction (qPCR) analysis has been increasingly used for detection of the causative fungus, Pseudogymnoascus destructans, in both bat- and eAuthorsMichelle Verant, Elizabeth A. Bohuski, Jeffrey M. Lorch, David S. BlehertExperimental infection of snakes with Ophidiomyces ophiodiicola causes pathological changes that typify snake fungal disease
Snake fungal disease (SFD) is an emerging skin infection of wild snakes in eastern North America. The fungus Ophidiomyces ophiodiicola is frequently associated with the skin lesions that are characteristic of SFD, but a causal relationship between the fungus and the disease has not been established. We experimentally infected captive-bred corn snakes (Pantherophis guttatus) in the laboratory withAuthorsJeffrey M. Lorch, Julia S. Lankton, Katrien Werner, Elizabeth A. Falendysz, Kevin McCurley, David S. BlehertTaqMan real-time polymerase chain reaction for detection of Ophidiomyces ophiodiicola, the fungus associated with snake fungal disease
Background Fungal skin infections associated with Ophidiomyces ophiodiicola, a member of the Chrysosporiumanamorph of Nannizziopsis vriesii (CANV) complex, have been linked to an increasing number of cases of snake fungal disease (SFD) in captive snakes around the world and in wild snake populations in eastern North America. The emergence of SFD in both captive and wild situations has led to an iAuthorsElizabeth A. Bohuski, Jeffrey M. Lorch, Kathryn M. Griffin, David S. BlehertAvian botulism type E in waterbirds of Lake Michigan, 2010–2013
During 2010 to 2013, waterbird mortality surveillance programs used a shared protocol for shoreline walking surveys performed June to November at three areas in northern Lake Michigan. In 2010 and 2012, 1244 total carcasses (0.8 dead bird/km walked) and 2399 total carcasses (1.2 dead birds/km walked), respectively, were detected. Fewer carcasses were detected in 2011 (353 total carcasses, 0.2 deadAuthorsJennifer G. Chipault, C. LeAnn White, David S. Blehert, Susan K. Jennings, Sean M. StromDirect detection of fungal siderophores on bats with white-nose syndrome via fluorescence microscopy-guided ambient ionization mass spectrometry
White-nose syndrome (WNS) caused by the pathogenic fungus Pseudogymnoascus destructans is decimating the populations of several hibernating North American bat species. Little is known about the molecular interplay between pathogen and host in this disease. Fluorescence microscopy ambient ionization mass spectrometry was used to generate metabolic profiles from the wings of both healthy and diseaseAuthorsSamantha J. Mascuch, Wilna J. Moree, Cheng-Chih Cheng-Chih Hsu, Gregory G. Turner, Tina L. Cheng, David S. Blehert, A. Marm Kilpatrick, Winifred F. Frick, Michael J. Meehan, Pieter C. Dorrestein, Lena GerwickThe fungus Trichophyton redellii sp. nov. causes skin infections that resemble white-nose syndrome of hibernating bats
Before the discovery of white-nose syndrome (WNS), a fungal disease caused by Pseudogymnoascus destructans, there were no reports of fungal skin infections in bats during hibernation. In 2011, bats with grossly visible fungal skin infections similar in appearance to WNS were reported from multiple sites in Wisconsin, USA, a state outside the known range of P. destructans and WNS at that time. TapeAuthorsJeffrey M. Lorch, Andrew M. Minnis, Carol U. Meteyer, Jennifer A. Redell, J. Paul White, Heather M. Kaarakka, Laura K. Muller, David L. Lindner, Michelle L. Verant, Valerie I. Shearn-Bochsler, David S. BlehertNon-USGS Publications**
Rickard, A.H., R.J. Palmer, Jr., D.S. Blehert, S.R. Campagna, M.F. Semmelhack, P.G. Egland, B.L. Bassler, and P.E. Kolenbrander. 2006. Autoinducer 2: a concentration-dependent signal for mutualistic bacterial biofilm growth. Molecular Microbiology 60: 1446-1456.Orville, A. M., L. Manning, D.S. Blehert, J.M. Studts, B.W. Matthews, B.G. Fox, and G.H. Chambliss. 2004. Crystallization and preliminary analysis of xenobiotic reductase A and ligand complexes from Pseudomonas putida II-B. Acta Crystallographica 60: 957-961.Orville, A. M., L. Manning, D.S. Blehert, B.G. Fox, and G.H. Chambliss. 2004. Crystallization and preliminary analysis of xenobiotic reductase B from Pseudomonas fluorescens I-C. Acta Crystallographica 60: 1289-1291.Blehert, D.S., R.J. Palmer, Jr., J.B. Xavier, J.S. Almeida, and P.E. Kolenbrander. 2003. Autoinducer-2 production by Streptococcus gordonii DL1 and the biofilm phenotype of a luxS mutant are influenced by nutritional conditions. Journal of Bacteriology 185: 4851-4860.Kolenbrander, P.E., R.F. Lerud, D.S. Blehert, P.G. Egland, J.S. Foster, and R.J. Palmer, Jr. 2003. The role of coaggregation in oral biofilm formation. In V. O’Flaherty, A. Moran, P. Lens and P. Stoodley (eds.), Biofilms in Medicine, Industry and Environmental Biotechnology, IWA Publishing, London, UK. p. 32-46.Kolenbrander, P.E., R.N. Andersen, D.S. Blehert, P.G. Egland, J.S. Foster, and R.J. Palmer, Jr. 2002. Communication among oral bacteria. Microbiology and Molecular Biology Reviews 66: 486-505.Blehert, D.S., B.G. Fox, and G.H. Chambliss. 1999. Cloning and sequence analysis of two Pseudomonas flavoprotein xenobiotic reductases. Journal of Bacteriology 181: 6254-6263.Blehert, D.S., K.L. Knoke, B.G. Fox, and G.H. Chambliss. 1997. Regioselectivity of nitroglycerin denitration by flavoprotein nitroester reductases purified from two Pseudomonas species. Journal of Bacteriology 179: 6912-6920.**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
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*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government