Skip to main content
U.S. flag

An official website of the United States government

Deborah D Iwanowicz, PhD

Dr. Deborah D. Iwanowicz is a research fish biologist that utilizes molecular diagnostics for research studies.

Her research principally focuses on targeted amplicon sequencing applied to topics such as: diet evaluation, pollen identification, pathogen identification and microbial community profiling. She is a principle investigator and collaborator on a number of projects that focus on the effects of contaminants on fish health, the significance of disease organisms in invasive species, profiling complex microbial community dynamics, as well as protocol and assay development. Her research includes that of molecular microbiology (parasitology, bacteriology, virology on fishes, amphibians, sea turtles, marine mammals, insects, plants, aquatic and terrestrial gastropods). She leads efforts in the NFHRL for method development on metagenomics analyses of diet from scat, stomach content, or regurgitated cough pellets. This work has been modified to identify plant species found in pollen, diets of mammals, birds, and aquatic animals. She also often focuses her efforts on threatened and endangered species.

Education and Certifications

  • Ph.D., 2007, University of Georgia

  • M.S., 1998, Purdue University

  • B.S., 1993, Purdue University

Science and Products

No Result Found
No results found.
Filter Total Items: 36

Establishing molecular methods to quantitatively profile gastric diet items of fish—Application to the invasive blue catfish (ictalurus furcatus)

Understanding the diet of invasive species helps researchers to more accurately assess the health, survivorship, growth, and stability of an invasive fish species, as well as their effects on native populations. Techniques capable of identifying multiple prey species from fish stomach contents have been developed. In this study, a multi-locus metabarcoding approach was used to identify fish and in
Authors
Deborah D. Iwanowicz, W. Bane Schill, Lakyn R. Sanders, Tim Groves, Mary C. Groves
By
Ecosystems Mission Area, Eastern Ecological Science Center, Chesapeake Bay Activities

Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola

The fountain darter Etheostoma fonticola (FOD) is a federally endangered fish listed under the US Endangered Species Act. Here, we identified and characterized a novel aquareovirus isolated from wild fountain darters inhabiting the San Marcos River. This virus was propagated in Chinook salmon embryo (CHSE)-214, rainbow trout gonad-2 and fathead minnow cells at 15°C. The epithelioma papulosum cypri
Authors
Luke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Teresa Lewis, Tom Brandt, Lakyn R. Sanders, Robert S. Cornman
By
Ecosystems Mission Area, Eastern Ecological Science Center, Fort Collins Science Center

A method for determining avian influenza virus hemagglutinin and neuraminidase subtype association

Methods for grouping specific avian influenza virus (AIV) hemagglutinin (HA) and neuraminidase (NA) subtype reverse-transcription polymerase chain reaction (RT-PCR) products into HA:NA subtypes when egg incubation is technically not feasible were evaluated. These approaches were adopted for use as post hoc methods after melt curve analysis. The methods are based on ratios obtained from amplicon co
Authors
Christopher A. Ottinger, Deborah D. Iwanowicz, Luke R. Iwanowicz, Cynthia R. Adams, Lakyn R. Sanders, Christine L. Densmore
By
Ecosystems Mission Area, Eastern Ecological Science Center

Exposure to synthetic hydraulic fracturing waste influences the mucosal bacterial community structure of the brook trout (Salvelinus fontinalis) epidermis

Production of natural gas using unconventional technologies has risen as demand for alternative fuels has increased. Impacts on the environment from waste generated from these processes are largely unexplored. In particular, the outcomes of organismal exposure to hydraulic fracturing waste have not been rigorously evaluated. We evaluated the effects of exposure to surrogate hydraulic fracturing wa
Authors
Heather S. Galbraith, Deborah D. Iwanowicz, Daniel E. Spooner, Luke R. Iwanowicz, David Keller, Paula M. Zelanko, Cynthia R. Adams
By
Ecosystems Mission Area, Eastern Ecological Science Center

Genomic evolution, recombination, and inter-strain diversity of chelonid alphaherpesvirus 5 from Florida and Hawaii green sea turtles with fibropapillomatosis

Chelonid alphaherpesvirus 5 (ChHV5) is a herpesvirus associated with fibropapillomatosis (FP) in sea turtles worldwide. Single-locus typing has previously shown differentiation between Atlantic and Pacific strains of this virus, with low variation within each geographic clade. However, a lack of multi-locus genomic sequence data hinders understanding of the rate and mechanisms of ChHV5 evolutionar
Authors
Cheryl L. Morrison, Luke R. Iwanowicz, Thierry M. Work, Elizabeth Fahsbender, Mya Breitbart, Cynthia R. Adams, Deborah D. Iwanowicz, Lakyn Sanders, Mathias Ackermann, Robert S. Cornman
By
Ecosystems Mission Area, Eastern Ecological Science Center, Fort Collins Science Center, National Wildlife Health Center

Using colony monitoring devices to evaluate the impacts of land use and nutritional value of forage on honey bee health

Colony monitoring devices used to track and assess the health status of honey bees are becoming more widely available and used by both beekeepers and researchers. These devices monitor parameters relevant to colony health at frequent intervals, often approximating real time. The fine-scale record of hive condition can be further related to static or dynamic features of the landscape, such as weath
Authors
Matthew Smart, Clint R.V. Otto, Robert S. Cornman, Deborah D. Iwanowicz
By
Ecosystems Mission Area, Species Management Research Program, Northern Prairie Wildlife Research Center

Endozoicomonas dominates the gill and intestinal content microbiomes of Mytilus edulis from Barnegat Bay, New Jersey

Blue mussels, Mytilus edulis, Linnaeus 1758 from southern Barnegat Bay, New Jersey were examined to determine the make-up of the normal blue mussel microbiome. Sequencing of 16S ribosomal DNA amplicons from gill and intestinal content microbiomes using the Illumina® MiSeq platform yielded 1,276,161 paired end sequence reads from the gill libraries and 1,092,333 paired end sequence reads from the
Authors
William B. Schill, Deborah D. Iwanowicz, Cynthia R. Adams
By
Ecosystems Mission Area, Eastern Ecological Science Center

Draft genome sequence of a picorna-like virus associated with gill tissue in clinically normal brook trout, Salvelinus fontinalis

Here, we report a draft genome sequence of a picorna-like virus associated with brook trout, Salvelinus fontinalis, gill tissue. The draft genome comprises 8,681 nucleotides, excluding the poly(A) tract, and contains two open reading frames. It is most similar to picorna-like viruses that infect invertebrates.
Authors
Luke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Heather S. Galbraith, Aaron Aunins, Robert S. Cornman
By
Ecosystems Mission Area, Eastern Ecological Science Center

RNA sequencing analysis of transcriptional change in the freshwater mussel Elliptio complanata after environmentally relevant sodium chloride exposure

To identify potential biomarkers of salt stress in a freshwater sentinel species, we examined transcriptional responses of the common mussel Elliptio complanata to controlled sodium chloride (NaCl) exposures. Ribonucleic acid sequencing (RNA-Seq) of mantle tissue identified 481 transcripts differentially expressed in adult mussels exposed to 2 ppt NaCl (1.2 ppt chloride) for 7 d, of which 290 had
Authors
Laura S. Robertson, Heather S. Galbraith, Deborah D. Iwanowicz, Carrie J. Blakeslee, Robert S. Cornman
By
Ecosystems Mission Area, Eastern Ecological Science Center

Low pathogenic avian influenza viruses in wild migratory waterfowl in a region of high poultry production, Delmarva, Maryland

Migratory waterfowl are natural reservoirs for low pathogenic avian influenza viruses (AIVs) and may contribute to the long-distance dispersal of these pathogens as well as spillover into domestic bird populations. Surveillance for AIVs is critical to assessing risks for potential spread of these viruses among wild and domestic bird populations. The Delmarva Peninsula on the east coast of the Unit
Authors
Diann J. Prosser, Christine L. Densmore, Larry J. Hindman, Deborah D. Iwanowicz, Christopher A. Ottinger, Luke R. Iwanowicz, Cindy P. Driscoll, Jessica L. Nagel
By
Ecosystems Mission Area, Eastern Ecological Science Center

Potential concerns with analytical Methods Used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA

Taxonomic identification of pollen has historically been accomplished via light microscopy but requires specialized knowledge and reference collections, particularly when identification to lower taxonomic levels is necessary. Recently, next-generation sequencing technology has been used as a cost-effective alternative for identifying bee-collected pollen; however, this novel approach has not been
Authors
Deborah D. Iwanowicz, William B. Schill, Deanna H. Olson, M. J. Adams, Christine L. Densmore, Robert S. Cornman, Cynthia R. Adams, Chester Figiel, Chauncey W. Anderson, Andrew R Blaustein, Tara Chestnut
By
Ecosystems Mission Area, Water Resources Mission Area, Eastern Ecological Science Center, Forest and Rangeland Ecosystem Science Center, Oregon Water Science Center

A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding

Taxonomic identification of pollen has historically been accomplished via light microscopy but requires specialized knowledge and reference collections, particularly when identification to lower taxonomic levels is necessary. Recently, next-generation sequencing technology has been used as a cost-effective alternative for identifying bee-collected pollen; however, this novel approach has not been
Authors
Matthew Smart, Robert S. Cornman, Deborah D. Iwanowicz, Margaret McDermott-Kubeczko, Jeff S Pettis, Marla S Spivak, Clint R.V. Otto
By
Ecosystems Mission Area, Species Management Research Program, Eastern Ecological Science Center, Fort Collins Science Center, Northern Prairie Wildlife Research Center

Non-USGS Publications**

Cartwright, D.D. 1998. Dietary lipid levels of juvenile yellow perch (Master’s Thesis). Purdue University, West Lafayette, IN.
Densmore, C. L., J. H. Schachte, V. S. Blazer, T. B. Waldrop, C. J. Petrie, A. Mack, M. V. Bature, P. S. Pooler, D.D. Cartwright and W. B. Schill. 2001. A comparison of susceptibility to Myxobolus cerebralis, the cause of whirling disease among Onchorhynchus mykiss strains in field and laboratory exposures. J. Aquatic Animal Health 13:220-227.
Gauthier, D. T., D.D. Cartwright, C. L. Densmore, V. S. Blazer, and C. A. Ottinger. 2003. Measurement of in vitro mitogenesis in fish: ELISA based detection of the thymidine analogue 5’-bromo-2’-deoxyuridine. Fish & Shellfish Immunology 14(4):279-288.
Blazer, V. S., Densmore, C. L.; Schill, W. B.; Cartwright, D.D., and Page, S. J. 2004. Comparative susceptibility of Atlantic salmon, lake trout and rainbow trout to Myxobolus cerebralis in controlled laboratory exposures. Diseases of Aquatic Organisms 58(1):27-34.
Iwanowicz, L., Griffin, A., Cartwright, D., and Blazer, V. 2006. Mortality and pathology associated in brown bullheads (Ameiurus nebulosus) naturally infected with Edwarsiella ictaluri under tank culture conditions. Diseases of Aquatic Organisms 70(3):219-225.
Blazer, V. S., L. R. Iwanowicz, D. D. Iwanowicz, D. R. Smith, J. A. Young, J. D. Hedrick, S. W. Foster, and S.J. Reeser. 2007. Intersex (Testicular oocytes) in Smallmouth bass Micropterus dolomieu from the Potomac River and selected nearby drainages. Journal of Aquatic Animal Health. 19: 242-253.
Iwanowicz, D.D. 2007. Effects of environmental stressors and anthropogenic disturbances on infections with myxozoan parasites (Doctoral dissertation). University of Georgia, Athens, Georgia. Print.
Iwanowicz, L. R., D. D. Iwanowicz, L. M. Pote, V. S. Blazer, and W. B. Schill. 2008. Morphology and 18S rDNA of Henneguya gurlei (Myxosporea) from Ameiurus nebulosus (Siluriformes) in North Carolina, USA. Journal of Parasitology 94:46-57.
Burdick, S.M., Ottinger, C., Brown, D.T., VanderKooi, S.P., Robertson, L., and Iwanowicz, D.. 2009. Distribution, health, and development of larval and juvenile Lost River and Shortnose suckers in the Williamson River Delta restoration project and Upper Klamath Lake, Oregon: 2008 Annual Data Summary: U.S. Geological Survey Open-File Report 2009-1287, 76 p.
Blazer, V.S., L.R. Iwanowicz, C.E. Starliper, and D.D. Iwanowicz. 2010. Mortality of Centrarchid fishes in the Potomac Drainage: Survey results and overview of potential contributing factors. Journal of Aquatic Animal Health. 22: 190-218.

1. Iwanowicz, D.D. 2011. Overview On the Effects of Parasites On Fish Health. Pages 176-184 in Cipriano, R.C., Bruckner, A.W. and Shchelkunov, I.S., editors. 2011. Bridging America and Russia with Shared Perspectives on Aquatic Animal Health Proceedings of the Third Bilateral Conference between Russia and the United States. 20 July 2009. Shepherdstown, West Virginia. Khaled bin Sultan Living Oceans Foundation, Landover, Maryland, USA.
https://www.lsc.usgs.gov/files/D%20Iwanowicz%202011.pdf
Walsh, HL, LR Iwanowicz, GW Glenney, D. D. Iwanowicz, VS Blazer. 2012. Description of Two New Gill Myxozoans from Smallmouth (Micropterus dolomieu) and Largemouth (M. salmoides) Bass. Journal of Parasitology. 98 415-422.
Iwanowicz, D. D., L.R. Iwanowicz, E.W. Howerth, W.B. Schill, V.S. Blazer, and R.L. Johnson. 2013. Characterization of a new Myxozoan Species (Myxozoa: Myxobolidae: Myxosporea) in Largescale Stonerollers (Campostoma oligolepis) from the Mobile River Basin (Alabama). Journal of Parasitology 99(1): 102-111.
Ciparis, S., D. D. Iwanowicz and J. R. Voshell. 2013. Relationships between nutrient enrichment, pleurocerid snail densities, and trematode infection rates in streams. Fish Biology 58 (7): 1392-1404.
Iwanowicz. D.D., L.R. Iwanowicz, N. Hitt, T.L. King. 2013. Differential expression profiles of miRNA in the Little Brown Bat (Myotis lucifugus) associated with White Nose affected and unaffected individuals. U.S. Geological Survey Open-File Report 2013–1099, 11 p., available only at http://pubs.usgs.gov/of/2013/1099
Blazer, V.S, D.D. Iwanowicz, H.L. Walsh, A.J. Sperry, L.R. Iwanowicz, D.A. Alvarez, R.A. Brightbill, G. Smith and R. Manning. 2014. Reproductive Health Indicators of Fishes from Pennsylvania Watersheds: Association with Chemicals of Emerging Concern. Environmental Monitoring and Assessment 186 (10): 6471-6491.
Morrison, C., M. Springmann, D. Iwanowicz, and C. Wade. 2014.Development of ten microsatellite loci in the intensive giant African land snail, Achatina (Lissachatina) fulica Bowdich, 1822. Conservation Genetics Resources. 19 September.
Chalupnicki, M., D. Dittman, C.E. Starliper, and D.D. Iwanowicz. 2015. Efficacy of Iodine for Disinfection of Lake Sturgeon Eggs from the St. Lawrence River, New York. North American Journal of Aquaculture 77: 82-89.
Iwanowicz, D, Sanders L, Schill W., Xayavong M, de Silva, A, Qvarnstrom Y, Smith T. 2015. Spread of the Rat Lungworm (Angiostrogylus cantonensis) in Giant African Land Snails (Lissachatina fulica) in Florida, USA. Journal of Wildlife Diseases 51(3): 749-753. doi: 10.7589/2014-06-160. Epub 2015 May 14.
Starliper, C.E., B.J. Watten, D.D. Iwanowicz, P.A. Green, N. L. Bassett, and C.R. Adams. 2015. Efficacy of pH Elevation as a Bactericidal Strategy for Treating Ballast Water of Freight Carriers. Journal of Advanced Research 6: 501-509.

Iwanowicz, L., V. Blazer, A. Pinkney, C. Guy, A. Major, K. Munney, S. Mierzykowski, S. Lingenfelser, A. Secord, T. Kubiak, C. Stern, C. Hahn, D. Iwanowicz, H. Walsh, and A. Sperry. 2015. Evidence of Estrogenic Endocrine Disruption in Smallmouth Bass and Largemouth Bass Inhabiting Northeast U.S. National Wildlife Refuge Waters: A Reconnaissance Study. Ecotoxicology and Environmental Safety 124: 50-59.
Densmore, C.L., L.R. Iwanowicz, A.P. Henderson, D.D. Iwanowicz and J.S. Odenkirk. 2015. Mycobacterial Infection in Northern Snakehead (Channa argus) from the Potomac River Catchment. Journal of Fish Diseases. Epub 2015.
Cornman, R.S., C.R.V. Otto, D. Iwanowicz, J.S. Pettis. 2015. Taxonomic characterization of honey bee (Apis mellifera) pollen foraging based on non-overlapping paired-end sequencing of nuclear ribosomal loci. Plos One
Iwanowicz, D., M.C. Black, V.S. Blazer, H. Zappia, and W. Bryant. 2016. Effects of urban land-use on largescale stonerollers in the Mobile River Basin, Birmingham, AL. Ecotoxicology 25:608-621.
Ottinger, C.A., C.L. Densmore, L.S. Robertson, D.D. Iwanowicz, S.P. VanderKooi. 2016. Transforming growth factor-β1 expression in endangered age-0 shortnose suckers (Chasmistes brevirostris) from Upper Klamath Lake, OR relative to histopathology, meristic, patial and temporal data. Fish and Shellfish Immunology 49:1-6.
Iwanowicz, D.D., A.G. Vandergast, R.S. Cornman, C.R. Adams, J.R. Kohn, R.N. Fisher, C.S. Brehme. 2016. Metabarcoding of fecal samples to determine herbivore diets: a case study of the endangered pacific pocket mouse. Plos One
Iwanowicz, D.D., W.B. Schill, D.H. Olson, M.J. Adams, C. Densmore, R.S.Cornman, C. Adams, C.Figiel, Jr., C.W. Anderson, A.R. Blaustein, and T. Chestnut. 2017. Potential concerns with the analytical methods used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA. Herpetological Review 48(2): 352-355.
Smart, M.D., R.S. Cornman, D.D. Iwanowicz, M. McDermott-Kubeczko, J.S. Pettis, M.S. Spivak, C.R.V. Otto. 2017. A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding. Environmental Entomology 46(1):38-49. DOI: 10.1093/ee/nvw159
Prosser, D.J., C.L.Densmore, L.J. Hindman, D.D. Iwanowicz, C.A. Ottinger, L.R. Iwanowicz, C.P. Driscoll, J.L. Nagel. 2017. Low pathogenic avian influenza viruses in wild migratory waterfowl in a region of high poultry production, Delmarva, Maryland. Avian Diseases 61(1): 128-134.

**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.

Science and Products

  • Science
  • Data
    No Result Found
  • Multimedia
    No results found.
  • Publications
    Filter Total Items: 36

    Establishing molecular methods to quantitatively profile gastric diet items of fish—Application to the invasive blue catfish (ictalurus furcatus)

    Understanding the diet of invasive species helps researchers to more accurately assess the health, survivorship, growth, and stability of an invasive fish species, as well as their effects on native populations. Techniques capable of identifying multiple prey species from fish stomach contents have been developed. In this study, a multi-locus metabarcoding approach was used to identify fish and in
    Authors
    Deborah D. Iwanowicz, W. Bane Schill, Lakyn R. Sanders, Tim Groves, Mary C. Groves
    By
    Ecosystems Mission Area, Eastern Ecological Science Center, Chesapeake Bay Activities

    Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola

    The fountain darter Etheostoma fonticola (FOD) is a federally endangered fish listed under the US Endangered Species Act. Here, we identified and characterized a novel aquareovirus isolated from wild fountain darters inhabiting the San Marcos River. This virus was propagated in Chinook salmon embryo (CHSE)-214, rainbow trout gonad-2 and fathead minnow cells at 15°C. The epithelioma papulosum cypri
    Authors
    Luke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Teresa Lewis, Tom Brandt, Lakyn R. Sanders, Robert S. Cornman
    By
    Ecosystems Mission Area, Eastern Ecological Science Center, Fort Collins Science Center

    A method for determining avian influenza virus hemagglutinin and neuraminidase subtype association

    Methods for grouping specific avian influenza virus (AIV) hemagglutinin (HA) and neuraminidase (NA) subtype reverse-transcription polymerase chain reaction (RT-PCR) products into HA:NA subtypes when egg incubation is technically not feasible were evaluated. These approaches were adopted for use as post hoc methods after melt curve analysis. The methods are based on ratios obtained from amplicon co
    Authors
    Christopher A. Ottinger, Deborah D. Iwanowicz, Luke R. Iwanowicz, Cynthia R. Adams, Lakyn R. Sanders, Christine L. Densmore
    By
    Ecosystems Mission Area, Eastern Ecological Science Center

    Exposure to synthetic hydraulic fracturing waste influences the mucosal bacterial community structure of the brook trout (Salvelinus fontinalis) epidermis

    Production of natural gas using unconventional technologies has risen as demand for alternative fuels has increased. Impacts on the environment from waste generated from these processes are largely unexplored. In particular, the outcomes of organismal exposure to hydraulic fracturing waste have not been rigorously evaluated. We evaluated the effects of exposure to surrogate hydraulic fracturing wa
    Authors
    Heather S. Galbraith, Deborah D. Iwanowicz, Daniel E. Spooner, Luke R. Iwanowicz, David Keller, Paula M. Zelanko, Cynthia R. Adams
    By
    Ecosystems Mission Area, Eastern Ecological Science Center

    Genomic evolution, recombination, and inter-strain diversity of chelonid alphaherpesvirus 5 from Florida and Hawaii green sea turtles with fibropapillomatosis

    Chelonid alphaherpesvirus 5 (ChHV5) is a herpesvirus associated with fibropapillomatosis (FP) in sea turtles worldwide. Single-locus typing has previously shown differentiation between Atlantic and Pacific strains of this virus, with low variation within each geographic clade. However, a lack of multi-locus genomic sequence data hinders understanding of the rate and mechanisms of ChHV5 evolutionar
    Authors
    Cheryl L. Morrison, Luke R. Iwanowicz, Thierry M. Work, Elizabeth Fahsbender, Mya Breitbart, Cynthia R. Adams, Deborah D. Iwanowicz, Lakyn Sanders, Mathias Ackermann, Robert S. Cornman
    By
    Ecosystems Mission Area, Eastern Ecological Science Center, Fort Collins Science Center, National Wildlife Health Center

    Using colony monitoring devices to evaluate the impacts of land use and nutritional value of forage on honey bee health

    Colony monitoring devices used to track and assess the health status of honey bees are becoming more widely available and used by both beekeepers and researchers. These devices monitor parameters relevant to colony health at frequent intervals, often approximating real time. The fine-scale record of hive condition can be further related to static or dynamic features of the landscape, such as weath
    Authors
    Matthew Smart, Clint R.V. Otto, Robert S. Cornman, Deborah D. Iwanowicz
    By
    Ecosystems Mission Area, Species Management Research Program, Northern Prairie Wildlife Research Center

    Endozoicomonas dominates the gill and intestinal content microbiomes of Mytilus edulis from Barnegat Bay, New Jersey

    Blue mussels, Mytilus edulis, Linnaeus 1758 from southern Barnegat Bay, New Jersey were examined to determine the make-up of the normal blue mussel microbiome. Sequencing of 16S ribosomal DNA amplicons from gill and intestinal content microbiomes using the Illumina® MiSeq platform yielded 1,276,161 paired end sequence reads from the gill libraries and 1,092,333 paired end sequence reads from the
    Authors
    William B. Schill, Deborah D. Iwanowicz, Cynthia R. Adams
    By
    Ecosystems Mission Area, Eastern Ecological Science Center

    Draft genome sequence of a picorna-like virus associated with gill tissue in clinically normal brook trout, Salvelinus fontinalis

    Here, we report a draft genome sequence of a picorna-like virus associated with brook trout, Salvelinus fontinalis, gill tissue. The draft genome comprises 8,681 nucleotides, excluding the poly(A) tract, and contains two open reading frames. It is most similar to picorna-like viruses that infect invertebrates.
    Authors
    Luke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Heather S. Galbraith, Aaron Aunins, Robert S. Cornman
    By
    Ecosystems Mission Area, Eastern Ecological Science Center

    RNA sequencing analysis of transcriptional change in the freshwater mussel Elliptio complanata after environmentally relevant sodium chloride exposure

    To identify potential biomarkers of salt stress in a freshwater sentinel species, we examined transcriptional responses of the common mussel Elliptio complanata to controlled sodium chloride (NaCl) exposures. Ribonucleic acid sequencing (RNA-Seq) of mantle tissue identified 481 transcripts differentially expressed in adult mussels exposed to 2 ppt NaCl (1.2 ppt chloride) for 7 d, of which 290 had
    Authors
    Laura S. Robertson, Heather S. Galbraith, Deborah D. Iwanowicz, Carrie J. Blakeslee, Robert S. Cornman
    By
    Ecosystems Mission Area, Eastern Ecological Science Center

    Low pathogenic avian influenza viruses in wild migratory waterfowl in a region of high poultry production, Delmarva, Maryland

    Migratory waterfowl are natural reservoirs for low pathogenic avian influenza viruses (AIVs) and may contribute to the long-distance dispersal of these pathogens as well as spillover into domestic bird populations. Surveillance for AIVs is critical to assessing risks for potential spread of these viruses among wild and domestic bird populations. The Delmarva Peninsula on the east coast of the Unit
    Authors
    Diann J. Prosser, Christine L. Densmore, Larry J. Hindman, Deborah D. Iwanowicz, Christopher A. Ottinger, Luke R. Iwanowicz, Cindy P. Driscoll, Jessica L. Nagel
    By
    Ecosystems Mission Area, Eastern Ecological Science Center

    Potential concerns with analytical Methods Used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA

    Taxonomic identification of pollen has historically been accomplished via light microscopy but requires specialized knowledge and reference collections, particularly when identification to lower taxonomic levels is necessary. Recently, next-generation sequencing technology has been used as a cost-effective alternative for identifying bee-collected pollen; however, this novel approach has not been
    Authors
    Deborah D. Iwanowicz, William B. Schill, Deanna H. Olson, M. J. Adams, Christine L. Densmore, Robert S. Cornman, Cynthia R. Adams, Chester Figiel, Chauncey W. Anderson, Andrew R Blaustein, Tara Chestnut
    By
    Ecosystems Mission Area, Water Resources Mission Area, Eastern Ecological Science Center, Forest and Rangeland Ecosystem Science Center, Oregon Water Science Center

    A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding

    Taxonomic identification of pollen has historically been accomplished via light microscopy but requires specialized knowledge and reference collections, particularly when identification to lower taxonomic levels is necessary. Recently, next-generation sequencing technology has been used as a cost-effective alternative for identifying bee-collected pollen; however, this novel approach has not been
    Authors
    Matthew Smart, Robert S. Cornman, Deborah D. Iwanowicz, Margaret McDermott-Kubeczko, Jeff S Pettis, Marla S Spivak, Clint R.V. Otto
    By
    Ecosystems Mission Area, Species Management Research Program, Eastern Ecological Science Center, Fort Collins Science Center, Northern Prairie Wildlife Research Center

    Non-USGS Publications**

    Cartwright, D.D. 1998. Dietary lipid levels of juvenile yellow perch (Master’s Thesis). Purdue University, West Lafayette, IN.
    Densmore, C. L., J. H. Schachte, V. S. Blazer, T. B. Waldrop, C. J. Petrie, A. Mack, M. V. Bature, P. S. Pooler, D.D. Cartwright and W. B. Schill. 2001. A comparison of susceptibility to Myxobolus cerebralis, the cause of whirling disease among Onchorhynchus mykiss strains in field and laboratory exposures. J. Aquatic Animal Health 13:220-227.
    Gauthier, D. T., D.D. Cartwright, C. L. Densmore, V. S. Blazer, and C. A. Ottinger. 2003. Measurement of in vitro mitogenesis in fish: ELISA based detection of the thymidine analogue 5’-bromo-2’-deoxyuridine. Fish & Shellfish Immunology 14(4):279-288.
    Blazer, V. S., Densmore, C. L.; Schill, W. B.; Cartwright, D.D., and Page, S. J. 2004. Comparative susceptibility of Atlantic salmon, lake trout and rainbow trout to Myxobolus cerebralis in controlled laboratory exposures. Diseases of Aquatic Organisms 58(1):27-34.
    Iwanowicz, L., Griffin, A., Cartwright, D., and Blazer, V. 2006. Mortality and pathology associated in brown bullheads (Ameiurus nebulosus) naturally infected with Edwarsiella ictaluri under tank culture conditions. Diseases of Aquatic Organisms 70(3):219-225.
    Blazer, V. S., L. R. Iwanowicz, D. D. Iwanowicz, D. R. Smith, J. A. Young, J. D. Hedrick, S. W. Foster, and S.J. Reeser. 2007. Intersex (Testicular oocytes) in Smallmouth bass Micropterus dolomieu from the Potomac River and selected nearby drainages. Journal of Aquatic Animal Health. 19: 242-253.
    Iwanowicz, D.D. 2007. Effects of environmental stressors and anthropogenic disturbances on infections with myxozoan parasites (Doctoral dissertation). University of Georgia, Athens, Georgia. Print.
    Iwanowicz, L. R., D. D. Iwanowicz, L. M. Pote, V. S. Blazer, and W. B. Schill. 2008. Morphology and 18S rDNA of Henneguya gurlei (Myxosporea) from Ameiurus nebulosus (Siluriformes) in North Carolina, USA. Journal of Parasitology 94:46-57.
    Burdick, S.M., Ottinger, C., Brown, D.T., VanderKooi, S.P., Robertson, L., and Iwanowicz, D.. 2009. Distribution, health, and development of larval and juvenile Lost River and Shortnose suckers in the Williamson River Delta restoration project and Upper Klamath Lake, Oregon: 2008 Annual Data Summary: U.S. Geological Survey Open-File Report 2009-1287, 76 p.
    Blazer, V.S., L.R. Iwanowicz, C.E. Starliper, and D.D. Iwanowicz. 2010. Mortality of Centrarchid fishes in the Potomac Drainage: Survey results and overview of potential contributing factors. Journal of Aquatic Animal Health. 22: 190-218.

    1. Iwanowicz, D.D. 2011. Overview On the Effects of Parasites On Fish Health. Pages 176-184 in Cipriano, R.C., Bruckner, A.W. and Shchelkunov, I.S., editors. 2011. Bridging America and Russia with Shared Perspectives on Aquatic Animal Health Proceedings of the Third Bilateral Conference between Russia and the United States. 20 July 2009. Shepherdstown, West Virginia. Khaled bin Sultan Living Oceans Foundation, Landover, Maryland, USA.
    https://www.lsc.usgs.gov/files/D%20Iwanowicz%202011.pdf
    Walsh, HL, LR Iwanowicz, GW Glenney, D. D. Iwanowicz, VS Blazer. 2012. Description of Two New Gill Myxozoans from Smallmouth (Micropterus dolomieu) and Largemouth (M. salmoides) Bass. Journal of Parasitology. 98 415-422.
    Iwanowicz, D. D., L.R. Iwanowicz, E.W. Howerth, W.B. Schill, V.S. Blazer, and R.L. Johnson. 2013. Characterization of a new Myxozoan Species (Myxozoa: Myxobolidae: Myxosporea) in Largescale Stonerollers (Campostoma oligolepis) from the Mobile River Basin (Alabama). Journal of Parasitology 99(1): 102-111.
    Ciparis, S., D. D. Iwanowicz and J. R. Voshell. 2013. Relationships between nutrient enrichment, pleurocerid snail densities, and trematode infection rates in streams. Fish Biology 58 (7): 1392-1404.
    Iwanowicz. D.D., L.R. Iwanowicz, N. Hitt, T.L. King. 2013. Differential expression profiles of miRNA in the Little Brown Bat (Myotis lucifugus) associated with White Nose affected and unaffected individuals. U.S. Geological Survey Open-File Report 2013–1099, 11 p., available only at http://pubs.usgs.gov/of/2013/1099
    Blazer, V.S, D.D. Iwanowicz, H.L. Walsh, A.J. Sperry, L.R. Iwanowicz, D.A. Alvarez, R.A. Brightbill, G. Smith and R. Manning. 2014. Reproductive Health Indicators of Fishes from Pennsylvania Watersheds: Association with Chemicals of Emerging Concern. Environmental Monitoring and Assessment 186 (10): 6471-6491.
    Morrison, C., M. Springmann, D. Iwanowicz, and C. Wade. 2014.Development of ten microsatellite loci in the intensive giant African land snail, Achatina (Lissachatina) fulica Bowdich, 1822. Conservation Genetics Resources. 19 September.
    Chalupnicki, M., D. Dittman, C.E. Starliper, and D.D. Iwanowicz. 2015. Efficacy of Iodine for Disinfection of Lake Sturgeon Eggs from the St. Lawrence River, New York. North American Journal of Aquaculture 77: 82-89.
    Iwanowicz, D, Sanders L, Schill W., Xayavong M, de Silva, A, Qvarnstrom Y, Smith T. 2015. Spread of the Rat Lungworm (Angiostrogylus cantonensis) in Giant African Land Snails (Lissachatina fulica) in Florida, USA. Journal of Wildlife Diseases 51(3): 749-753. doi: 10.7589/2014-06-160. Epub 2015 May 14.
    Starliper, C.E., B.J. Watten, D.D. Iwanowicz, P.A. Green, N. L. Bassett, and C.R. Adams. 2015. Efficacy of pH Elevation as a Bactericidal Strategy for Treating Ballast Water of Freight Carriers. Journal of Advanced Research 6: 501-509.

    Iwanowicz, L., V. Blazer, A. Pinkney, C. Guy, A. Major, K. Munney, S. Mierzykowski, S. Lingenfelser, A. Secord, T. Kubiak, C. Stern, C. Hahn, D. Iwanowicz, H. Walsh, and A. Sperry. 2015. Evidence of Estrogenic Endocrine Disruption in Smallmouth Bass and Largemouth Bass Inhabiting Northeast U.S. National Wildlife Refuge Waters: A Reconnaissance Study. Ecotoxicology and Environmental Safety 124: 50-59.
    Densmore, C.L., L.R. Iwanowicz, A.P. Henderson, D.D. Iwanowicz and J.S. Odenkirk. 2015. Mycobacterial Infection in Northern Snakehead (Channa argus) from the Potomac River Catchment. Journal of Fish Diseases. Epub 2015.
    Cornman, R.S., C.R.V. Otto, D. Iwanowicz, J.S. Pettis. 2015. Taxonomic characterization of honey bee (Apis mellifera) pollen foraging based on non-overlapping paired-end sequencing of nuclear ribosomal loci. Plos One
    Iwanowicz, D., M.C. Black, V.S. Blazer, H. Zappia, and W. Bryant. 2016. Effects of urban land-use on largescale stonerollers in the Mobile River Basin, Birmingham, AL. Ecotoxicology 25:608-621.
    Ottinger, C.A., C.L. Densmore, L.S. Robertson, D.D. Iwanowicz, S.P. VanderKooi. 2016. Transforming growth factor-β1 expression in endangered age-0 shortnose suckers (Chasmistes brevirostris) from Upper Klamath Lake, OR relative to histopathology, meristic, patial and temporal data. Fish and Shellfish Immunology 49:1-6.
    Iwanowicz, D.D., A.G. Vandergast, R.S. Cornman, C.R. Adams, J.R. Kohn, R.N. Fisher, C.S. Brehme. 2016. Metabarcoding of fecal samples to determine herbivore diets: a case study of the endangered pacific pocket mouse. Plos One
    Iwanowicz, D.D., W.B. Schill, D.H. Olson, M.J. Adams, C. Densmore, R.S.Cornman, C. Adams, C.Figiel, Jr., C.W. Anderson, A.R. Blaustein, and T. Chestnut. 2017. Potential concerns with the analytical methods used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA. Herpetological Review 48(2): 352-355.
    Smart, M.D., R.S. Cornman, D.D. Iwanowicz, M. McDermott-Kubeczko, J.S. Pettis, M.S. Spivak, C.R.V. Otto. 2017. A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding. Environmental Entomology 46(1):38-49. DOI: 10.1093/ee/nvw159
    Prosser, D.J., C.L.Densmore, L.J. Hindman, D.D. Iwanowicz, C.A. Ottinger, L.R. Iwanowicz, C.P. Driscoll, J.L. Nagel. 2017. Low pathogenic avian influenza viruses in wild migratory waterfowl in a region of high poultry production, Delmarva, Maryland. Avian Diseases 61(1): 128-134.

    **Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.

  • Web Tools
  • News