Keiner, R., A. Rusznyak, D. M. Akob, S. Hanf, K. Küsel, J. Popp, and T. Frosch. 2013. Raman spectroscopy – an innovative and versatile tool to follow the respirational activity and carbonate biomineralization of important cave bacteria. Analytical Chemistry, 85(18): 8708–8714 (DOI: 10.1021/ac401699d).
Denise M Akob, Ph.D.
Biography
Dr. Denise M. Akob is a geomicrobiologist whose research focuses on understanding how microorganisms impact their environment and biogeochemical cycles. She investigates both contaminated and pristine environments. Her research focuses primarily on understanding microbial impacts on Cold War Biogeochemistry (uranium and chlorinated solvent contamination); energy production (either by mitigating degradation of contaminants (from hydraulic fracturing or oil spills) or by enhancing natural gas production)); and carbon cycling. Dr. Akob joined the U.S. Geological Survey in 2012 and is a research scientist in the Water Mission Area's Laboratory Analytical Services Division, Strategic Laboratory Sciences Branch. For more information, please visit the Reston Microbiology Lab website.
Education
- Ph.D. 2008, Florida State University, Department of Oceanography, Advisor: Dr. Joel E. Kostka
- B.A. 2002, St. Mary’s College of Maryland, Major: Biology
Professional Experience
- Affiliated Professor, Department of Biology at George Mason University, 2017-current.
- Affiliated Professor, Department of Geology, Appalachian State University, 2017- present.
- Adjunct Graduate Faculty, University of Alabama, Department of Biological Sciences, 2016-present.
- Affiliated Professor, Department of Geography and Planning, Appalachian State University, 2016-current.
- Affiliated Professor, Environmental Science and Policy Department at George Mason University, 2013-current.
- Research Microbiologist, U.S. Geological Survey, Water Mission Area, Reston Microbiology Laboratory. 2012-current
- Postdoctoral Research Fellow, Friedrich-Schiller University Jena. 2010-2012
- Marie Curie Postdoctoral Research Fellow, Friedrich-Schiller University Jena. 2008-2010
Professional Activities
- Associate Editor, Biogeosciences, 2016-present.
- Editorial Board Member, FEMS Microbiology Ecology, 2016-present.
- Editorial Board Member, Applied and Environmental Microbiology, January 1, 2018- December 31, 2020.
- President, Washington, D.C. Branch of the American Society for Microbiology, 2019-present.
- President-Elect, Washington, D.C. Branch of the American Society for Microbiology, 2017-2019.
- USGS Community for Data Integration’s Bioinformatics Community of Practice, co-organizer, 2017-present.
- USGS Eastern Water Science Series, co-organizer, 2017-present.
- Applied and Environmental Microbiology Gordon Research Seminar Mentor, July 15-16, 2017.
- USGS NRP-EB Weekly Science Series, co-organizer (2013-2017)
- Geobiology Theme Chair, Goldschmidt 2015 25th Anniversary Meeting
- Co-Chair for Session T50. Microbial Hydrocarbon Formation and Biodegradation: Organisms, Pathways, Environmental Limitations, and Isotope Signatures, 2015 Geological Society of America Annual Meeting, Baltimore, MD
Awards
- USGS Early Career Leadership Award, 2017
Science and Products
Date published: May 19, 2017
Status: Completed Two Scientists Receive Early Career Excellence in Leadership Award
The U.S. Geological Survey (USGS) 2016 Early Career Excellence in Leadership Award was given to Dr. Denise M. Akob and Dr. Karl B. Haase. Drs. Akob and Haase have demonstrated outstanding leadership through their scientific accomplishments and service to the USGS.
Contacts: Denise M Akob, Ph.D., Karl B Haase, Ph.D.
Date published: April 12, 2017
Status: Completed Trace Levels of Organic Chemicals Limited to Local Reaches of a Stream near an Oil and Gas Wastewater Disposal Facility
Organic contaminants that were present in Wolf Creek near a wastewater disposal facility were not evident farther downstream where Wolf Creek enters the New River. Wolf Creek and the New River are used for drinking water and recreational purposes.
Contacts: William H Orem, Ph.D., Denise M Akob, Ph.D.
Date published: February 24, 2017
Status: Completed Examining Shifts in Stream Microbial Communities Exposed to Oil and Gas Wastewaters
Shifts in the overall microbial community structure were present in stream sediments that contained chemicals associated with unconventional oil and gas wastewaters. This work is part of a long-term study designed to understand persistence of chemicals from oil and gas wastewaters in sediments and water and how those factors might be related to exposures and adverse health effects, if any, on...
Contacts: Denise M Akob, Ph.D., Isabelle M Cozzarelli
Attribution: Environmental Health, Toxic Substances Hydrology Program
Date published: May 9, 2016
Status: Completed Indication of Unconventional Oil and Gas Wastewaters Found in Local Surface Waters
Evidence indicating the presence of wastewaters from unconventional oil and gas (UOG) production was found in surface waters and surficial sediments near an UOG disposal facility in West Virginia.
Contacts: Denise M Akob, Ph.D., Isabelle M Cozzarelli
Attribution: Environmental Health, Toxic Substances Hydrology Program
Date published: June 17, 2015
Status: Completed Microbiology and Chemistry of Waters Produced from Hydraulic Fracking—A Case Study
A new U.S. Geological Survey (USGS) study determined that the microbiology and organic chemistry of produced waters varied widely among hydraulically fractured shale gas wells in north-central Pennsylvania.
Contacts: Denise M Akob, Ph.D., Isabelle M Cozzarelli
Attribution: Environmental Health, Toxic Substances Hydrology Program
Year Published: 2020
Binning singletons: Mentoring through networking at ASM microbe 2019
The American Society for Microbiology (ASM) national conference, Microbe, is the flagship meeting for microbiologists across the globe. The presence of roughly 10,000 attendees provides enormous opportunities for networking and learning. However, such a large meeting can be intimidating to many, especially early career scientists, students, those...
James, Joseph B.; Gunn, Amanda L.; Akob, Denise M.
Year Published: 2019
Draft genome sequence of the Mn(II)-oxidizing bacterium Oxalobacteraceae sp. AB_14
Biological Mn(II) oxidation produces reactive manganese oxides that help to mitigate metal contamination in the environment. Here we present the genome of Oxalobacteraceae sp. AB_14, a species of Mn(II)-oxidizing bacteria (MOB) notable for its ability to catalyze Mn oxidation at low pH (5.5).
Bushman, Timothy (Contractor); Akob, Denise M.; Bohu, Tsing; Beyer, Andrea; Fabisch, Maria; Woyke, Tanja; Shapiro, Nicole; Lapidus, Alla; Klenk, Hans-Peter; Küsel, Kirsten
Year Published: 2019
Phylogenetic techniques in geomicrobiology
Molecular biological techniques have revolutionized the field of geomicrobiology by providing researchers with robust techniques for identifying microorganisms and characterizing microbial communities in a wide variety of environments. These techniques have freed researchers from the constraints of classical culture-based microbiology and allowed...
Akob, Denise M.; Mumford, Adam C.; Dunlop, Darren S.; Poret-Peterson, Amisha T.
Year Published: 2019
Organic geochemistry and toxicology of a stream impacted by unconventional oil and gas wastewater disposal operations
Water and sediment extracts samples were analyzed for extractable hydrocarbons by gas chromatography/mass spectrometry (GC/MS) using an Agilent (Agilent Technologies, Palo Alto, CA, USA) 7890 series GC and 5975 electron ionization (EI) mass selective detector (MSD) operated in scan mode. Agilent ChemStation software was used for data acquisition...
Orem, William H.; Varonka, Matthew S.; Crosby, Lynn M.; Haase, Karl B.; Loftin, Keith A.; Hladik, Michelle; Akob, Denise M.; Tatu, Calin; Mumford, Adam C.; Jaeschke, Jeanne B.; Bates, Anne L.; Schell, Tiffani; Cozzarelli, Isabelle M.
Year Published: 2018
Microbial community composition of a hydrocarbon reservoir 40 years after a CO2 enhanced oil recovery flood
Injecting CO2 into depleted oil reservoirs to extract additional crude oil is a common enhanced oil recovery (CO2-EOR) technique. However, little is known about how in situ microbial communities may be impacted by CO2 flooding, or if any permanent microbiological changes occur after flooding has ceased. Formation water was...
Shelton, Jenna L.; Andrews, Robert S.; Akob, Denise M.; DeVera, Christina A.; Mumford, Adam C.; McCray, John E.; McIntosh, Jennifer C.
Year Published: 2018
An initial comparison of pesticides and amphibian pathogens between natural and created wetlands in the New Jersey Pinelands, 2014–16
A study conducted by the U.S. Geological Survey, in cooperation with the New Jersey Pinelands Commission and Montclair State University, was designed to compare pesticide concentrations and the presence and prevalence of amphibian pathogens between natural ponds and two types of created wetlands, excavated ponds and stormwater basins, throughout...
Smalling, Kelly L.; Bunnell, John F.; Cohl, Jonathan; Romanok, Kristin M.; Hazard, Lisa; Monsen, Kirsten; Akob, Denise M.; Hansen, Angela M.; Hladik, Michelle L.; Abdallah, Nicole; Ahmed, Quratulain; Assan, Araba; De Parsia, Matthew; Griggs, Amaryl; McWayne-Holmes, Megan; Patel, Naisargi; Sanders, Corey; Shrestha, Yesha; Stout, Sean M.; Williams, BriannaAttribution: New Jersey Water Science Center, Water Resources
View Citation
Smalling, K.L., Bunnell, J.F., Cohl, J., Romanok, K.M., Hazard, L., Monsen, K., Akob, D.M., Hansen, A., Hladik, M.L., Abdallah, N., Ahmed, Q., Assan, A., De Parsia, M., Griggs, A., McWayne-Holmes, M., Patel, N., Sanders, C., Shrestha, Y., Stout, S., and Williams, B., 2018, An initial comparison of pesticides and amphibian pathogens between natural and created wetlands in the New Jersey Pinelands, 2014–16: U.S. Geological Survey Open-File Report 2018–1077, 18 p., https://doi.org/10.3133/ofr20181077.
Year Published: 2018
Acetylenotrophy: A hidden but ubiquitous microbial metabolism?
Acetylene (IUPAC name: ethyne) is a colorless, gaseous hydrocarbon, composed of two triple bonded carbon atoms attached to hydrogens (C2H2). When microbiologists and biogeochemists think of acetylene, they immediately think of its use as an inhibitory compound of certain microbial processes and a tracer for nitrogen fixation. However, what is less...
Akob, Denise M.; Sutton, John M.; Fierst, Janna L.; Haase, Karl B.; Baesman, Shaun; Luther, George; Miller, Laurence G.; Oremland, Ronald S.Attribution: Environmental Health, Environmental Health, Water Resources, Toxic Substances Hydrology Program, United States of America
View Citation
Denise M Akob, John M Sutton, Janna L Fierst, Karl B Haase, Shaun Baesman, George W Luther, Laurence G Miller, Ronald S Oremland; Acetylenotrophy: a hidden but ubiquitous microbial metabolism?, FEMS Microbiology Ecology, Volume 94, Issue 8, 1 August 2018, fiy103, https://doi.org/10.1093/femsec/fiy103.
Year Published: 2018
Common hydraulic fracturing fluid additives alter the structure and function of anaerobic microbial communities
The development of unconventional oil and gas (UOG) resources results in the production of large volumes of wastewater containing a complex mixture of hydraulic fracturing chemical additives and components from the formation. The release of these wastewaters into the environment poses potential risks that are poorly understood. Microbial...
Mumford, Adam C.; Akob, Denise M.; Klinges, J. Grace; Cozzarelli, Isabelle M.Attribution: Environmental Health, Environmental Health, Water Resources, Energy & Wildlife, Toxic Substances Hydrology Program, United States of America, Advanced Research Computing (ARC)
View Citation
Mumford AC, Akob DM, Klinges JG, Cozzarelli IM. 2018. Common hydraulic fracturing fluid additives alter the structure and function of anaerobic microbial communities. Appl Environ Microbiol 84:e02729-17
Year Published: 2017
Degradation of crude 4-MCHM (4-methylcyclohexanemethanol) in sediments from Elk River, West Virginia
In January 2014, approximately 37 800 L of crude 4-methylcyclohexanemethanol (crude MCHM) spilled into the Elk River, West Virginia. To understand the long-term fate of 4-MCHM, we conducted experiments under environmentally relevant conditions to assess the potential for the 2 primary compounds in crude MCHM (1) to undergo biodegradation and (2)...
Cozzarelli, Isabelle M.; Akob, Denise M.; Baedecker, Mary Jo; Spencer, Tracey; Jaeschke, Jeanne B.; Dunlap, Darren S.; Mumford, Adam C.; Poret-Peterson, Amisha T.; Chambers, Douglas B.Attribution: Environmental Health, Environmental Health, Water Resources, Toxic Substances Hydrology Program, United States of America
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Cozzarelli, I. M., D. M. Akob, M. J. Baedecker, T. Spencer, J. Jaeschke, D. S. Dunlap, A. C. Mumford, A. T. Poret-Peterson, D. B. Chambers. 2017. Degradation of MCHM (4-methylcyclohexanemethanol) in Sediments from Elk River, West Virginia. Environmental Science and Technology, 51(21): 12139-12145.
Year Published: 2017
Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93
Acetylene (C2H2) is a trace constituent of the present Earth's oxidizing atmosphere, reflecting a mixture of terrestrial and marine emissions from anthropogenic, biomass-burning, and unidentified biogenic sources. Fermentation of acetylene was serendipitously discovered during C2H2 block assays of N2O reductase, and Pelobacter acetylenicus was...
Akob, Denise M.; Baesman, Shaun; Sutton, John M.; Fierst, Janna L.; Mumford, Adam; Shrestha, Yesha; Poret-Peterson, Amisha T.; Bennett, Stacy; Dunlap, Darren S.; Haase, Karl B.; Oremland, Ronald S.View Citation
Akob, D. M.; Baesman, S. M.; Sutton, J. M.; Fierst, J. L.; Mumford, A. C.; Shrestha, Y.; Poret-Peterson, A. T.; Bennett, S.; Dunlap, D. S.; Haase, K. B.; Oremland, R. S., (2017) Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93. Applied and Environmental Microbiology, 83(17): e01198-17, 10.1128/aem.01198-17.
Year Published: 2017
Archaeal diversity and CO2 fixers in carbonate-/siliciclastic-rock groundwater ecosystems
Groundwater environments provide habitats for diverse microbial communities, and although Archaea usually represent a minor fraction of communities, they are involved in key biogeochemical cycles. We analysed the archaeal diversity within a mixed carbonate-rock/siliciclastic-rock aquifer system, vertically from surface soils to subsurface...
Lazar, Cassandre Sara; Stoll, Wenke; Lehmann, Robert; Herrmann, Martina; Schwab, Valérie F.; Akob, Denise M.; Nawaz, Ali; Wubet, Tesfaye; Buscot, François; Totsche, Kai-Uwe; Küsel, KirstenAttribution: Water Resources,
View Citation
Lazar, C. S.; Stoll, W.; Lehmann, R.; Herrmann, M.; Schwab, V. F.; Akob, D. M.; Nawaz, A.; Wubet, T.; Buscot, F.; Totsche, K.-U.; Küsel, K., Archaeal diversity and CO2 fixers in carbonate-/siliciclastic-rock groundwater ecosystems. Archaea 2017, 2017, 1-13, https://doi.org/10.1155/2017/2136287
Year Published: 2017
Shifts in microbial community structure and function in surface waters impacted by unconventional oil and gas wastewater revealed by metagenomics
Unconventional oil and gas (UOG) production produces large quantities of wastewater with complex geochemistry and largely uncharacterized impacts on surface waters. In this study, we assessed shifts in microbial community structure and function in sediments and waters upstream and downstream from a UOG wastewater disposal facility. To do this,...
Fahrenfeld, N.L.; Reyes, Hannah Delos; Eramo, Alessia; Akob, Denise M.; Mumford, Adam; Cozzarelli, Isabelle M.View Citation
Fahrenfeld, N.L., H. Delos Reyes, A. Eramo, D. M. Akob, A. Mumford, and I.M. Cozzarelli. 2017. Shifts in microbial community structure and function in surface waters impacted by unconventional oil and gas wastewaters revealed by metagenomics, Science of the Total Environment, 580: 1205–1213, http://dx.doi.org/10.1016/j.scitotenv.2016.12.079.
Pre-USGS Publications
Gray, S. M., D. M. Akob, S. J. Green, and J. E. Kostka. 2012. The predictability of bacterial composition within the Sarracenia purpurea model system: local scale differences and relationships with the other members of the food web. PLoS One 7(12): e50969.
Akob, D. M.*, S. H. Lee*, M. Sheth, K. Küsel, D. B. Watson, A.V. Palumbo, J.E. Kostka, and K.-J. Chin. 2012. Gene expression correlates with process rates quantified for sulfate- and Fe(III)-reducing bacteria in U(VI)-contaminated sediments. Frontiers in Terrestrial Microbiology, 3:280. *Equal contribution.
Risse-Buhl, U., M. Herrmann, D. M. Akob, P. Geesink, N. Pizani, W. Schönborn, K.-U. Totsche and K. Küsel. 2012 Phagotrophic protist diversity in the groundwater of a karstic aquifer - morphological and molecular analysis. Journal of Eukaryotic Microbiology, 60(5): 467–479 (DOI: 10.1111/jeu.12054.
Rusznyák, A., D. M. Akob, S. Nietzsche, K. Eusterhues, K. U. Totsche, T. R. Neu, T. Frosch, J. Popp, R. Keiner, J. Geletneky, L. Katzschmann, E.-D. Schulze, and K. Küsel. 2012. Calcite biomineralization by bacterial isolates from the recently discovered pristine karstic Herrenberg Cave. Applied and Environmental Microbiology 78(4): 1157–1167.
Akob, D. M. and K. Küsel. 2011. Where microorganisms meet rocks in the Earth's Critical Zone. Biogeosciences 8, 3531-3543.
Akob, D. M., L. Kerkhof, K. Küsel, D. B. Watson, A. V. Palumbo, and J. E. Kostka. 2011. Linking specific heterotrophic bacterial populations to bioreduction of uranium and nitrate using stable isotope probing in contaminated subsurface sediments. Applied and Environmental Microbiology. 77(22):8197-8200
Burkhardt, E.-M., S. Bischoff, D. M. Akob, G. Büchel, and K. Küsel. 2011. Heavy metal tolerance of Fe(III)-reducing microbial communities in a contaminated creek bank soil. Applied and Environmental Microbiology 77(9): 3132-3136.
Lu, S., S. Gischkat, M. Reiche, D. M. Akob, K. B. Hallberg, and K. Küsel. 2010. Ecophysiology of Fe-cycling Bacteria in Acidic Sediments. Applied and Environmental Microbiology 76 (24): 8174-8183.
Vishnivetskaya, T. A., C. C. Brandt, A. S. Madden, M. S. Drake, J. E. Kostka, D. M. Akob, K. Küsel, and A. V. Palumbo. 2010. Microbial Community Changes in Response to Ethanol or Methanol Amendments for U(VI) Reduction. Applied and Environmental Microbiology 76(17): 5728-5735.
Sitte, J., D. M. Akob, C. Kaufmann, K. Finster, D. Banerjee, E.-M. Burkhardt, J. E. Kostka, A. Scheinost, Georg Büchel and K. Küsel. 2010. Microbial Links between Sulfate Reduction and Metal Retention in Uranium and Heavy Metal-contaminated Soil. Applied and Environmental Microbiology 76(10): 3143–3152.
Green, S. J., O. Prakash, T. M. Gihring, D. M. Akob, P. Jasrotia, P. M. Jardine, D. B. Watson, S. D. Brown, A. V. Palumbo and J. E. Kostka. 2010. Denitrifying bacteria from the terrestrial subsurface exposed to mixed waste contamination. Applied and Environmental Microbiology 76(10): 3244–3254.
Burkhardt, E.-M., D. M. Akob, S. Bischoff, J. Sitte, J. E. Kostka, D. Banerjee, A. C. Scheinost, K. Küsel. 2010. Impact of Biostimulated Redox Processes on Metal Dynamics in an Iron-rich Creek Soil of a former Uranium Mining Area. Environmental Science & Technology 44(1): 177-183.
O. Prakash, T.M. Gihring, D.D. Dalton, K.-J. Chin, S.J. Green, D.M. Akob, G. Wanger, J.E. Kostka. 2010. Geobacter daltonii sp. nov., an iron(III)- and uranium(VI)-reducing bacterium isolated from the shallow subsurface exposed to mixed heavy metal and hydrocarbon contamination. International Journal of Systematic and Evolutionary Microbiology 60: 546-553.
D. M. Akob, H. J. Mills, T. M. Gihring, L. Kerkhof, J. W. Stucki, Kuk-Jeong Chin, Kirsten Kuesel, Anthony V. Palumbo, David B. Watson, and Joel E. Kostka. 2008. Functional diversity and electron donor dependence of microbial populations capable of U(VI) reduction in radionuclide contaminated subsurface sediments. Applied and Environmental Microbiology 74: 3159-3170.
Blöthe, M., D.M. Akob, K. Walter, J.E. Kostka, H.L. Drake, and K. Küsel. 2008. Heterogeneity of Fe(III)-reducing microorganisms in coal-mining lake sediments caused by a pH gradient. Applied and Environmental Microbiology 74(4): 1019–102.
D. M. Akob, H. J. Mills, D. L. Swofford, J. E. Kostka. 2007. Metabolically-Active Microbial Communities in Uranium-Contaminated Subsurface Sediments. FEMS Microbiology Ecology 59: 95-107.
Date published: May 9, 2016
Evidence of Unconventional Oil and Gas Wastewater Found in Surface Waters near Underground Injection Site
These are the first published studies to demonstrate water-quality impacts to a surface stream due to activities at an unconventional oil and gas wastewater deep well injection disposal site.
Date published: May 11, 2015
The Chemistry of Waters that Follow from Fracking: A Case Study
In a study of 13 hydraulically fractured shale gas wells in north-central Pennsylvania, USGS researchers found that the microbiology and organic chemistry of the produced waters varied widely from well to well.