Dorothy Chase
Dorothy is a microbiologist at the Western Fisheries Research Center.
Science and Products
Filter Total Items: 16
Genetic effects of ELISA-based segregation for control of bacterial kidney disease in Chinook salmon (Oncorhynchus tshawytscha)
We evaluated genetic variation in ability of Chinook salmon (Oncorhynchus tshawytscha) to resist two bacterial pathogens: Renibacterium salmoninarum, the agent of bacterial kidney disease (BKD), and Listonella anguillarum, an agent of vibriosis. After measuring R. salmoninarum antigen in 499 adults by enzyme-linked immunosorbent assay (ELISA), we mated each of 12 males with high or low antigen lev
Authors
J.J. Hard, D.G. Elliott, R.J. Pascho, D.M. Chase, L.K. Park, J. R. Winton, D.E. Campton
Comparison of traditional and molecular methods for detection of Renibacterium salmoninarum
Bacterial kidney disease (BKD) caused by Renibacterium salmoninarum occurs in most parts of the world where wild or cultured salmonid fishes are present. Several extensive reviews have been written on the pathogen and the disease (Fryer and Sanders, 1981; Austin and Austin, 1987; Elliott et al., 1989; Evelyn, 1993; Evenden et al., 1993; Fryer and Lannan, 1993). Bacterial kidney disease can cause s
Authors
R.J. Pascho, D.G. Elliott, D.M. Chase
Development of a nested polymerase chain reaction for amplification of a sequence of the p57 gene of Renibacterium salmoninarum that provides a highly sensitive method for detection of the bacterium in salmonid kidney
Nucleic acid-based assays have shown promise for diagnosing Renibacterium salmoninarum in tissues and body fluids of salmonids. DeVelopment of a nested polymerase chain reaction (PCR) method to detect a 320 bp DNA segment of the gene encoding the p57 protein of R. salmoninarum is described. Whereas a conventional PCR for a 383 bp segment of the p57 gene reliably detected 1000 R. salmoninarum cells
Authors
D.M. Chase, R.J. Pascho
Comparison of the membrane-filtration fluorescent antibody test, the enzyme-linked immunosorbent assay, and the polymerase chain reaction to detect Renibacterium salmoninarum in salmon ovarian fluid
Ovarian fluid samples from naturally infected chinook salmon (Oncorhynchus tshawytscha) were examined for the presence of Renibacterium salmoninarum by the membrane-filtration fluorescent antibody test (MF-FAT), an antigen capture enzyme-linked immunosorbent assay (ELISA), and a nested polymerase chain reaction (PCR). On the basis of the MF-FAT, 64% (66/103) samples contained detectable levels of
Authors
Ronald J. Pascho, Dorothy M. Chase, Constance L. McKibben
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Filter Total Items: 16
Genetic effects of ELISA-based segregation for control of bacterial kidney disease in Chinook salmon (Oncorhynchus tshawytscha)
We evaluated genetic variation in ability of Chinook salmon (Oncorhynchus tshawytscha) to resist two bacterial pathogens: Renibacterium salmoninarum, the agent of bacterial kidney disease (BKD), and Listonella anguillarum, an agent of vibriosis. After measuring R. salmoninarum antigen in 499 adults by enzyme-linked immunosorbent assay (ELISA), we mated each of 12 males with high or low antigen levAuthorsJ.J. Hard, D.G. Elliott, R.J. Pascho, D.M. Chase, L.K. Park, J. R. Winton, D.E. CamptonComparison of traditional and molecular methods for detection of Renibacterium salmoninarum
Bacterial kidney disease (BKD) caused by Renibacterium salmoninarum occurs in most parts of the world where wild or cultured salmonid fishes are present. Several extensive reviews have been written on the pathogen and the disease (Fryer and Sanders, 1981; Austin and Austin, 1987; Elliott et al., 1989; Evelyn, 1993; Evenden et al., 1993; Fryer and Lannan, 1993). Bacterial kidney disease can cause sAuthorsR.J. Pascho, D.G. Elliott, D.M. ChaseDevelopment of a nested polymerase chain reaction for amplification of a sequence of the p57 gene of Renibacterium salmoninarum that provides a highly sensitive method for detection of the bacterium in salmonid kidney
Nucleic acid-based assays have shown promise for diagnosing Renibacterium salmoninarum in tissues and body fluids of salmonids. DeVelopment of a nested polymerase chain reaction (PCR) method to detect a 320 bp DNA segment of the gene encoding the p57 protein of R. salmoninarum is described. Whereas a conventional PCR for a 383 bp segment of the p57 gene reliably detected 1000 R. salmoninarum cellsAuthorsD.M. Chase, R.J. PaschoComparison of the membrane-filtration fluorescent antibody test, the enzyme-linked immunosorbent assay, and the polymerase chain reaction to detect Renibacterium salmoninarum in salmon ovarian fluid
Ovarian fluid samples from naturally infected chinook salmon (Oncorhynchus tshawytscha) were examined for the presence of Renibacterium salmoninarum by the membrane-filtration fluorescent antibody test (MF-FAT), an antigen capture enzyme-linked immunosorbent assay (ELISA), and a nested polymerase chain reaction (PCR). On the basis of the MF-FAT, 64% (66/103) samples contained detectable levels ofAuthorsRonald J. Pascho, Dorothy M. Chase, Constance L. McKibben