Using the 384-well plate format, a single zebrafish embryo is tested in each well. This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under a fluorescein isothiocyanate (FITC) filter.
Jessica K Leet, PhD
Dr. Jessica Leet is a Research Toxicologist at the Columbia Environmental Research Center.
Her current research is on effects of environmental contaminants on fish, with an emphasis on endocrine disruption during fish development and reproduction. Typically her research involves evaluation of multiple levels of biological organization, including analysis of gene and protein expression, plasma hormones, histology, growth, and reproduction.
Science and Products
Prior Exposure to Estrogen Enabled Bass to Better Survive A Bacterial Infection, but With an Energetic Price Tag
Summary of the published study: Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass ( Micropterus salmoides ) to bacterial infection (Leet et al. in review)
Effects of Early Life Stage Exposure of Largemouth Bass to Atrazine or a Model Estrogen (17α-Ethinylestradiol)
Early development is a sensitive window for estrogenic endocrine disruption in largemouth bass. Atrazine treatments caused changes to gene expression patterns that were both similar to estrogen and distinct from estrogen.
Contaminant-related Activities in Support of Client Agencies in the Department of the Interior
We undertake many research activities in support of the mission of the Department of the Interior. One such activity is to evaluate the potential hazard and risk of environmental contaminants to natural resources.
Computer-Assisted Sperm Motion Analysis in Measuring Reproductive Effects in Bass
The objective of this research is to assess the sensitivity of female and male LMB reproductive capabilities at the time of year when they are physiologically preparing for spawning season (also known as gonad recrudescence).
Using the 384-well plate format, a single zebrafish embryo is tested
Using the 384-well plate format, a single zebrafish embryo is tested in each well. This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under a fluorescein isothiocyanate (FITC) filter.
Molecular Devices ImageXpress† High-Content Imaging System
Molecular Devices ImageXpress† High-Content Imaging System
Molecular Devices ImageXpress† High-Content Imaging System
Molecular Devices ImageXpress† High-Content Imaging System
Molecular Devices ImageXpress† High-Content Imaging System that can generate more than 25,000 images in less than 5 hours of automated image acquisition.
Molecular Devices ImageXpress† High-Content Imaging System that can generate more than 25,000 images in less than 5 hours of automated image acquisition.
This is an example of a 72 hour post fertilization
This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under transmitted light. The same imaging system with was used.
This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under transmitted light. The same imaging system with was used.
Molecular Devices ImageXpress† High-Content Imaging System
High-Content Screening Laboratory — Columbia, Missouri. Molecular Devices ImageXpress† High-Content Imaging System
High-Content Screening Laboratory — Columbia, Missouri. Molecular Devices ImageXpress† High-Content Imaging System
TZebrafish developmental cardiovascular toxicity high-content screen
The zebrafish developmental cardiovascular toxicity high-content screening assay uses a 384-well plate format to maximize number of treatments and replicates possible on each plate.
The zebrafish developmental cardiovascular toxicity high-content screening assay uses a 384-well plate format to maximize number of treatments and replicates possible on each plate.
Science and Products
- Science
Prior Exposure to Estrogen Enabled Bass to Better Survive A Bacterial Infection, but With an Energetic Price Tag
Summary of the published study: Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass ( Micropterus salmoides ) to bacterial infection (Leet et al. in review)Effects of Early Life Stage Exposure of Largemouth Bass to Atrazine or a Model Estrogen (17α-Ethinylestradiol)
Early development is a sensitive window for estrogenic endocrine disruption in largemouth bass. Atrazine treatments caused changes to gene expression patterns that were both similar to estrogen and distinct from estrogen.Contaminant-related Activities in Support of Client Agencies in the Department of the Interior
We undertake many research activities in support of the mission of the Department of the Interior. One such activity is to evaluate the potential hazard and risk of environmental contaminants to natural resources.Computer-Assisted Sperm Motion Analysis in Measuring Reproductive Effects in Bass
The objective of this research is to assess the sensitivity of female and male LMB reproductive capabilities at the time of year when they are physiologically preparing for spawning season (also known as gonad recrudescence). - Data
- Multimedia
Using the 384-well plate format, a single zebrafish embryo is tested
Using the 384-well plate format, a single zebrafish embryo is tested in each well. This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under a fluorescein isothiocyanate (FITC) filter.
Using the 384-well plate format, a single zebrafish embryo is tested in each well. This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under a fluorescein isothiocyanate (FITC) filter.
Molecular Devices ImageXpress† High-Content Imaging SystemMolecular Devices ImageXpress† High-Content Imaging System
Molecular Devices ImageXpress† High-Content Imaging System
Molecular Devices ImageXpress† High-Content Imaging SystemMolecular Devices ImageXpress† High-Content Imaging System that can generate more than 25,000 images in less than 5 hours of automated image acquisition.
Molecular Devices ImageXpress† High-Content Imaging System that can generate more than 25,000 images in less than 5 hours of automated image acquisition.
This is an example of a 72 hour post fertilizationThis is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under transmitted light. The same imaging system with was used.
This is an example of a 72 hour post fertilization fli1:egfp zebrafish (3 millimeters long) imaged under transmitted light. The same imaging system with was used.
Molecular Devices ImageXpress† High-Content Imaging SystemHigh-Content Screening Laboratory — Columbia, Missouri. Molecular Devices ImageXpress† High-Content Imaging System
High-Content Screening Laboratory — Columbia, Missouri. Molecular Devices ImageXpress† High-Content Imaging System
TZebrafish developmental cardiovascular toxicity high-content screenThe zebrafish developmental cardiovascular toxicity high-content screening assay uses a 384-well plate format to maximize number of treatments and replicates possible on each plate.
The zebrafish developmental cardiovascular toxicity high-content screening assay uses a 384-well plate format to maximize number of treatments and replicates possible on each plate.
- Publications