Jodi S Blum
Jodi Blum is a Physical Scientist for the USGS Water Mission Area.
Science and Products
Filter Total Items: 18
Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucumán, Argentina
Brevibacterium linens AE038-8, isolated from As-contaminated groundwater in Tucumán (Argentina), is highly resistant to arsenic oxyanions, being able to tolerate up to 1 M As(V) and 75 mM As(III) in a complex medium. Strain AE038-8 was also able to reduce As(V) to As(III) when grown in complex medium but paradoxically it could not do this in a defined minimal medium with sodium acetate and ammoniu
Authors
Daniela Maizel, Jodi S. Blum, Marcela A. Ferrero, Sagar M. Utturkar, Steven D. Brown, Barry P. Rosen, Ronald S. Oremland
A microbial arsenic cycle in sediments of an acidic mine impoundment: Herman Pit, Clear Lake, California
The involvement of prokaryotes in the redox reactions of arsenic occurring between its +5 [arsenate; As(V)] and +3 [arsenite; As(III)] oxidation states has been well established. Most research to date has focused upon circum-neutral pH environments (e.g., freshwater or estuarine sediments) or arsenic-rich “extreme” environments like hot springs and soda lakes. In contrast, relatively little work h
Authors
Jodi S. Blum, Shelley McCann, S. Bennett, Laurence G. Miller, J. R. Stolz, B. Stoneburner, C. Saltikov, Ronald S. Oremland
Microbiological reduction of Sb(V) in anoxic freshwater sediments
Microbiological reduction of millimolar concentrations of Sb(V) to Sb(III) was observed in anoxic sediments from two freshwater settings: (1) a Sb- and As-contaminated mine site (Stibnite Mine) in central Idaho and 2) an uncontaminated suburban lake (Searsville Lake) in the San Francisco Bay Area. Rates of Sb(V) reduction in anoxic sediment microcosms and enrichment cultures were enhanced by amend
Authors
Ronald S. Oremland, Thomas R. Kulp, Laurence G. Miller, Franco Braiotta, Samuel M. Webb, Benjamin D Kocar, Jodi S. Blum
Desulfohalophilus alkaliarsenatis gen. nov., sp. nov., an extremely halophilic sulfate- and arsenate-respiring bacterium from Searles Lake, California
A haloalkaliphilic sulfate-respiring bacterium, strain SLSR-1, was isolated from a lactate-fed stable enrichment culture originally obtained from the extreme environment of Searles Lake, California. The isolate proved capable of growth via sulfate-reduction over a broad range of salinities (125–330 g/L), although growth was slowest at salt-saturation. Strain SLSR-1 was also capable of growth via d
Authors
Jodi Switzer Blum, Thomas R. Kulp, Sukkyun Han, Brian Lanoil, Chad W. Saltikov, John F. Stolz, Laurence G. Miller, Ronald S. Oremland
A bacterium that can grow by using arsenic instead of phosphorus
Life is mostly composed of the elements carbon, hydrogen, nitrogen, oxygen, sulfur, and phosphorus. Although these six elements make up nucleic acids, proteins, and lipids and thus the bulk of living matter, it is theoretically possible that some other elements in the periodic table could serve the same functions. Here, we describe a bacterium, strain GFAJ-1 of the Halomonadaceae, isolated from Mo
Authors
Felisa Wolfe-Simon, Jodi S. Blum, T.R. Kulp, Gordon W. Rattray, S.E. Hoeft, J. Pett-Ridge, J.F. Stolz, S.M. Webb, P.K. Weber, P.C.W. Davies, A.D. Anbar, R.S. Oremland
Microbiology: A microbial arsenic cycle in a salt-saturated, extreme environment
Searles Lake is a salt-saturated, alkaline brine unusually rich in the toxic element arsenic. Arsenic speciation changed from arsenate [As(V)] to arsenite [As(III)] with sediment depth. Incubated anoxic sediment slurries displayed dissimilatory As(V)-reductase activity that was markedly stimulated by H2 or sulfide, whereas aerobic slurries had rapid As(III)-oxidase activity. An anaerobic, extremel
Authors
R.S. Oremland, T.R. Kulp, J.S. Blum, S.E. Hoeft, S. Baesman, L.G. Miller, J.F. Stolz
Structural and spectral features of selenium nanospheres produced by Se-respiring bacteria
Certain anaerobic bacteria respire toxic selenium oxyanions and in doing so produce extracellular accumulations of elemental selenium [Se(0)]. We examined three physiologically and phylogenetically diverse species of selenate- and selenite-respiring bacteria, Sulfurospirillum barnesii, Bacillus selenitireducens, and Selenihalanaerobacter shriftii, for the occurrence of this phenomenon. When grown
Authors
R.S. Oremland, M.J. Herbel, J.S. Blum, S. Langley, T.J. Beveridge, P.M. Ajayan, T. Sutto, A.V. Ellis, S. Curran
Reduction of elemental selenium to selenide: Experiments with anoxic sediments and bacteria that respire Se-oxyanions
A selenite-respiring bacterium, Bacillus selenitireducens, produced significant levels of Se(-II) (as aqueous HSe−) when supplied with Se(0). B. selenitireducens was also able to reduce selenite [Se(IV)] through Se(0) to Se(-II). Reduction of Se(0) by B. selenitireducens was more rapid in cells grown on colloidal sulfur [S(0)] or Se(IV) as their electron acceptor than for cell lines grown on fumar
Authors
M.J. Herbel, J.S. Blum, R.S. Oremland, S.E. Borglin
Dissimilatory arsenate reductase activity and arsenate-respiring bacteria in bovine rumen fluid, hamster feces, and the termite hindgut
Bovine rumen fluid and slurried hamster feces completely reduced millimolar levels of arsenate to arsenite upon incubation under anoxic conditions. This activity was strongly inhibited by autoclaving or aerobic conditions, and partially inhibited by tungstate or chloramphenicol. The rate of arsenate reduction was faster in feces from a population of arsenate-watered (100 ppm) hamsters compared to
Authors
M.J. Herbel, Blum J. Switzer, S.E. Hoeft, S.M. Cohen, L.L. Arnold, J. Lisak, J.F. Stolz, R.S. Oremland
Selenihalanaerobacter shriftii gen. nov., sp. nov., a halophilic anaerobe from Dead Sea sediments that respires selenate
We isolated an obligately anaerobic halophilic bacterium from the Dead Sea that grew by respiration of selenate. The isolate, designated strain DSSe-1, was a gram-negative, non-motile rod. It oxidized glycerol or glucose to acetate+CO2 with concomitant reduction of selenate to selenite plus elemental selenium. Other electron acceptors that supported anaerobic growth on glycerol were nitrate and tr
Authors
Blum J. Switzer, J.F. Stolz, A. Oren, R.S. Oremland
Bacterial dissimilatory reduction of arsenate and sulfate in meromictic Mono Lake, California
The stratified (meromictic) water column of alkaline and hypersaline Mono Lake, California, contains high concentrations of dissolved inorganic arsenic (~200 ??mol/L). Arsenic speciation changes from arsenate [As (V)] to arsenite [As (III)] with the transition from oxic surface waters (misolimnion) to anoxic bottom waters (monimolimnion). A radioassay was devised to measure the reduction of 73As (
Authors
R.S. Oremland, P.R. Dowdle, S. Hoeft, J.O. Sharp, J.K. Schaefer, L.G. Miller, Blum J. Switzer, R. L. Smith, N.S. Bloom, D. Wallschlaeger
Simultaneous reduction of nitrate and selenate by cell suspensions of selenium-respiring bacteria
Washed-cell suspensions of Sulfurospirillum barnesiireduced selenate [Se(VI)] when cells were cultured with nitrate, thiosulfate, arsenate, or fumarate as the electron acceptor. When the concentration of the electron donor was limiting, Se(VI) reduction in whole cells was approximately fourfold greater in Se(VI)-grown cells than was observed in nitrate-grown cells; correspondingly, nitrate reducti
Authors
R.S. Oremland, J.S. Blum, A.B. Bindi, P.R. Dowdle, M. Herbel, J.F. Stolz
Science and Products
- Publications
Filter Total Items: 18
Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucumán, Argentina
Brevibacterium linens AE038-8, isolated from As-contaminated groundwater in Tucumán (Argentina), is highly resistant to arsenic oxyanions, being able to tolerate up to 1 M As(V) and 75 mM As(III) in a complex medium. Strain AE038-8 was also able to reduce As(V) to As(III) when grown in complex medium but paradoxically it could not do this in a defined minimal medium with sodium acetate and ammoniuAuthorsDaniela Maizel, Jodi S. Blum, Marcela A. Ferrero, Sagar M. Utturkar, Steven D. Brown, Barry P. Rosen, Ronald S. OremlandA microbial arsenic cycle in sediments of an acidic mine impoundment: Herman Pit, Clear Lake, California
The involvement of prokaryotes in the redox reactions of arsenic occurring between its +5 [arsenate; As(V)] and +3 [arsenite; As(III)] oxidation states has been well established. Most research to date has focused upon circum-neutral pH environments (e.g., freshwater or estuarine sediments) or arsenic-rich “extreme” environments like hot springs and soda lakes. In contrast, relatively little work hAuthorsJodi S. Blum, Shelley McCann, S. Bennett, Laurence G. Miller, J. R. Stolz, B. Stoneburner, C. Saltikov, Ronald S. OremlandMicrobiological reduction of Sb(V) in anoxic freshwater sediments
Microbiological reduction of millimolar concentrations of Sb(V) to Sb(III) was observed in anoxic sediments from two freshwater settings: (1) a Sb- and As-contaminated mine site (Stibnite Mine) in central Idaho and 2) an uncontaminated suburban lake (Searsville Lake) in the San Francisco Bay Area. Rates of Sb(V) reduction in anoxic sediment microcosms and enrichment cultures were enhanced by amendAuthorsRonald S. Oremland, Thomas R. Kulp, Laurence G. Miller, Franco Braiotta, Samuel M. Webb, Benjamin D Kocar, Jodi S. BlumDesulfohalophilus alkaliarsenatis gen. nov., sp. nov., an extremely halophilic sulfate- and arsenate-respiring bacterium from Searles Lake, California
A haloalkaliphilic sulfate-respiring bacterium, strain SLSR-1, was isolated from a lactate-fed stable enrichment culture originally obtained from the extreme environment of Searles Lake, California. The isolate proved capable of growth via sulfate-reduction over a broad range of salinities (125–330 g/L), although growth was slowest at salt-saturation. Strain SLSR-1 was also capable of growth via dAuthorsJodi Switzer Blum, Thomas R. Kulp, Sukkyun Han, Brian Lanoil, Chad W. Saltikov, John F. Stolz, Laurence G. Miller, Ronald S. OremlandA bacterium that can grow by using arsenic instead of phosphorus
Life is mostly composed of the elements carbon, hydrogen, nitrogen, oxygen, sulfur, and phosphorus. Although these six elements make up nucleic acids, proteins, and lipids and thus the bulk of living matter, it is theoretically possible that some other elements in the periodic table could serve the same functions. Here, we describe a bacterium, strain GFAJ-1 of the Halomonadaceae, isolated from MoAuthorsFelisa Wolfe-Simon, Jodi S. Blum, T.R. Kulp, Gordon W. Rattray, S.E. Hoeft, J. Pett-Ridge, J.F. Stolz, S.M. Webb, P.K. Weber, P.C.W. Davies, A.D. Anbar, R.S. OremlandMicrobiology: A microbial arsenic cycle in a salt-saturated, extreme environment
Searles Lake is a salt-saturated, alkaline brine unusually rich in the toxic element arsenic. Arsenic speciation changed from arsenate [As(V)] to arsenite [As(III)] with sediment depth. Incubated anoxic sediment slurries displayed dissimilatory As(V)-reductase activity that was markedly stimulated by H2 or sulfide, whereas aerobic slurries had rapid As(III)-oxidase activity. An anaerobic, extremelAuthorsR.S. Oremland, T.R. Kulp, J.S. Blum, S.E. Hoeft, S. Baesman, L.G. Miller, J.F. StolzStructural and spectral features of selenium nanospheres produced by Se-respiring bacteria
Certain anaerobic bacteria respire toxic selenium oxyanions and in doing so produce extracellular accumulations of elemental selenium [Se(0)]. We examined three physiologically and phylogenetically diverse species of selenate- and selenite-respiring bacteria, Sulfurospirillum barnesii, Bacillus selenitireducens, and Selenihalanaerobacter shriftii, for the occurrence of this phenomenon. When grownAuthorsR.S. Oremland, M.J. Herbel, J.S. Blum, S. Langley, T.J. Beveridge, P.M. Ajayan, T. Sutto, A.V. Ellis, S. CurranReduction of elemental selenium to selenide: Experiments with anoxic sediments and bacteria that respire Se-oxyanions
A selenite-respiring bacterium, Bacillus selenitireducens, produced significant levels of Se(-II) (as aqueous HSe−) when supplied with Se(0). B. selenitireducens was also able to reduce selenite [Se(IV)] through Se(0) to Se(-II). Reduction of Se(0) by B. selenitireducens was more rapid in cells grown on colloidal sulfur [S(0)] or Se(IV) as their electron acceptor than for cell lines grown on fumarAuthorsM.J. Herbel, J.S. Blum, R.S. Oremland, S.E. BorglinDissimilatory arsenate reductase activity and arsenate-respiring bacteria in bovine rumen fluid, hamster feces, and the termite hindgut
Bovine rumen fluid and slurried hamster feces completely reduced millimolar levels of arsenate to arsenite upon incubation under anoxic conditions. This activity was strongly inhibited by autoclaving or aerobic conditions, and partially inhibited by tungstate or chloramphenicol. The rate of arsenate reduction was faster in feces from a population of arsenate-watered (100 ppm) hamsters compared toAuthorsM.J. Herbel, Blum J. Switzer, S.E. Hoeft, S.M. Cohen, L.L. Arnold, J. Lisak, J.F. Stolz, R.S. OremlandSelenihalanaerobacter shriftii gen. nov., sp. nov., a halophilic anaerobe from Dead Sea sediments that respires selenate
We isolated an obligately anaerobic halophilic bacterium from the Dead Sea that grew by respiration of selenate. The isolate, designated strain DSSe-1, was a gram-negative, non-motile rod. It oxidized glycerol or glucose to acetate+CO2 with concomitant reduction of selenate to selenite plus elemental selenium. Other electron acceptors that supported anaerobic growth on glycerol were nitrate and trAuthorsBlum J. Switzer, J.F. Stolz, A. Oren, R.S. OremlandBacterial dissimilatory reduction of arsenate and sulfate in meromictic Mono Lake, California
The stratified (meromictic) water column of alkaline and hypersaline Mono Lake, California, contains high concentrations of dissolved inorganic arsenic (~200 ??mol/L). Arsenic speciation changes from arsenate [As (V)] to arsenite [As (III)] with the transition from oxic surface waters (misolimnion) to anoxic bottom waters (monimolimnion). A radioassay was devised to measure the reduction of 73As (AuthorsR.S. Oremland, P.R. Dowdle, S. Hoeft, J.O. Sharp, J.K. Schaefer, L.G. Miller, Blum J. Switzer, R. L. Smith, N.S. Bloom, D. WallschlaegerSimultaneous reduction of nitrate and selenate by cell suspensions of selenium-respiring bacteria
Washed-cell suspensions of Sulfurospirillum barnesiireduced selenate [Se(VI)] when cells were cultured with nitrate, thiosulfate, arsenate, or fumarate as the electron acceptor. When the concentration of the electron donor was limiting, Se(VI) reduction in whole cells was approximately fourfold greater in Se(VI)-grown cells than was observed in nitrate-grown cells; correspondingly, nitrate reductiAuthorsR.S. Oremland, J.S. Blum, A.B. Bindi, P.R. Dowdle, M. Herbel, J.F. Stolz