John T. Lisle, Ph.D.
My research is focused on characterizing how microbes influence the geochemistry and carbon and nutrient cycling in surface water, ground water and coastal marine water and associated sediment systems thru the application of phylogenetics, microbial energetics and stable isotopes and radiolabeled substrates.
Professional Experience
2004-present Microbial Ecologist, USGS Center for Coastal & Watershed Studies, St. Petersburg, FL
2002-present Affiliate Graduate Faculty in the University of South Florida’s Biology Department
2002-2009 Assistant Courtesy Professor in the University of South Florida’s College of Marine Sciences
2002-2004 USGS Mendenhall Fellow, Center for Coastal & Watershed Studies, St. Petersburg, FL
2001-2002 Microbial Ecologist, NASA, Astrobiology Institute for the Study of Biomarkers, Johnson Space Center, Houston
2000-2001 Research Microbiologist, Lockheed Martin, NASA Astrobiology Institute for Biomarkers, Johnson Space Center, Houston
1998-2000 Assistant Research Professor, Department of Microbiology, Montana State University
1996-1998 Post-doctoral research fellow at Montana State University/Dr. Gordon McFeters, Department of Microbiology
Education and Certifications
1996 Ph.D., University of South Florida, College of Public Health
1983 M.S., Eastern Kentucky University, Department of Biology
1978 B.S., Eastern Kentucky University, Department of Biology
Affiliations and Memberships*
Collaborating scientist (2005-2007). The influence on microbial activities on arsenic mobilization from Floridan aquifer material (Dr. John Arthur/FLDEP).
Collaborating scientist (2005-2007). Microbial chemotaxis in hydrocarbon contaminated aquifers (Dr. Ron Harvey/USGS NRP).
Collaborating scientist (2004-Present). Characterization of bacterial and bacteriophage dynamics in ice and selected glacial melt streams and permanently ice-covered lakes in the Antarctic Dry Valleys
Science and Products
USGS Arctic Ocean Carbon Cruise 2012: Field Activity L-01-12-AR to collect carbon data in the Arctic Ocean, August-September 2012
Aragonite saturation states and nutrient fluxes in coral reef sediments in Biscayne National Park, FL, USA
U.S. Geological Survey Karst Interest Group Proceedings, Carlsbad, New Mexico, April 29-May 2, 2014
Evaluation of coral pathogen growth rates after exposure to atmospheric African dust samples
Survival of bacterial indicators and the functional diversity of native microbial communities in the Floridan aquifer system, south Florida
AMAP Assessment 2013: Arctic Ocean acidification
Baseline monitoring of the western Arctic Ocean estimates 20% of the Canadian Basin surface waters are undersaturated with respect to aragonite
USGS Arctic Ocean carbon cruise 2011: field activity H-01-11-AR to collect carbon data in the Arctic Ocean, August - September 2011
Characterization of sediments from the Gulf of Mexico and Atlantic shorelines, Texas to Florida
A survey of microbial community diversity in marine sediments impacted by petroleum hydrocarbons from the Gulf of Mexico and Atlantic shorelines, Texas to Florida
A survey of alterations in microbial community diversity in marine sediments in response to oil from the Deepwater Horizon spill: Northern Gulf of Mexico shoreline, Texas to Florida
When a habitat freezes solid: Microorganisms over-winter within the ice column of a coastal Antarctic lake
Science and Products
- Science
- Data
- Multimedia
- Publications
Filter Total Items: 37
USGS Arctic Ocean Carbon Cruise 2012: Field Activity L-01-12-AR to collect carbon data in the Arctic Ocean, August-September 2012
From August 25 to September 27, 2012, the United States Coast Guard Cutter (USCGC) Healy was part of an Extended Continental Shelf Project to determine the limits of the extended continental shelf in the Arctic. On a non-interference basis, a USGS ocean acidification team participated on the cruise to collect baseline water data in the Arctic. The collection of data extended from coastal waters neAuthorsLisa L. Robbins, Jonathan Wynn, Paul O. Knorr, Bogdan Onac, John T. Lisle, Katherine Y. McMullen, Kimberly K. Yates, Robert H. Byrne, Xuewu LiuAragonite saturation states and nutrient fluxes in coral reef sediments in Biscayne National Park, FL, USA
Some coral reefs, such as patch reefs along the Florida Keys reef tract, are not showing significant reductions in calcification rates in response to ocean acidification. It has been hypothesized that this recalcitrance is due to local buffering effects from biogeochemical processes driven by seagrasses. We investigated the influence that pore water nutrients, dissolved inorganic carbon (DIC) andAuthorsJohn T. Lisle, Christopher D. Reich, Robert B. HalleyU.S. Geological Survey Karst Interest Group Proceedings, Carlsbad, New Mexico, April 29-May 2, 2014
Karst aquifer systems are present throughout parts of the United States and some of its territories, and have developed in carbonate rocks (primarily limestone and dolomite) that span an interval of time encompassing more than 550 million years. The depositional environments, diagenetic processes, post-depositional tectonic events, and geochemical weathering processes that form karst aquifers areAuthorsEve L. Kuniansky, Lawrence E. SpanglerEvaluation of coral pathogen growth rates after exposure to atmospheric African dust samples
Laboratory experiments were conducted to assess if exposure to atmospheric African dust stimulates or inhibits the growth of four putative bacterial coral pathogens. Atmospheric dust was collected from a dust-source region (Mali, West Africa) and from Saharan Air Layer masses over downwind sites in the Caribbean [Trinidad and Tobago and St. Croix, U.S. Virgin Islands (USVI)]. Extracts of dust sampAuthorsJohn T. Lisle, Virginia H. Garrison, Michael A. GraySurvival of bacterial indicators and the functional diversity of native microbial communities in the Floridan aquifer system, south Florida
The Upper Floridan aquifer in the southern region of Florida is a multi-use, regional scale aquifer that is used as a potable water source and as a repository for passively recharged untreated surface waters, and injected treated surface water and wastewater, industrial wastes, including those which contain greenhouse gases (for example, carbon dioxide). The presence of confined zones within the FAuthorsJohn T. LisleAMAP Assessment 2013: Arctic Ocean acidification
This assessment report presents the results of the 2013 AMAP Assessment of Arctic Ocean Acidification (AOA). This is the first such assessment dealing with AOA from an Arctic-wide perspective, and complements several assessments that AMAP has delivered over the past ten years concerning the effects of climate change on Arctic ecosystems and people. The Arctic Monitoring and Assessment PrograBaseline monitoring of the western Arctic Ocean estimates 20% of the Canadian Basin surface waters are undersaturated with respect to aragonite
Marine surface waters are being acidified due to uptake of anthropogenic carbon dioxide, resulting in surface ocean areas of undersaturation with respect to carbonate minerals, including aragonite. In the Arctic Ocean, acidification is expected to occur at an accelerated rate with respect to the global oceans, but a paucity of baseline data has limited our understanding of the extent of Arctic undAuthorsLisa L. Robbins, Jonathan G. Wynn, John T. Lisle, Kimberly K. Yates, Paul O. Knorr, Robert H. Byrne, Xuewu Liu, Mark C. Patsavas, Kumiko Azetsu-Scott, Taro TakahashiUSGS Arctic Ocean carbon cruise 2011: field activity H-01-11-AR to collect carbon data in the Arctic Ocean, August - September 2011
Carbon dioxide (CO2) in the atmosphere is absorbed at the surface of the ocean by reacting with seawater to form a weak, naturally occurring acid called carbonic acid. As atmospheric carbon dioxide increases, the concentration of carbonic acid in seawater also increases, causing a decrease in ocean pH and carbonate mineral saturation states, a process known as ocean acidification. The oceans haveAuthorsLisa L. Robbins, Kimberly K. Yates, Paul O. Knorr, Jonathan Wynn, John Lisle, Brian J. Buczkowski, Barbara Moore, Larry Mayer, Andrew Armstrong, Robert H. Byrne, Xuewu LiuCharacterization of sediments from the Gulf of Mexico and Atlantic shorelines, Texas to Florida
In response to the Deepwater Horizon oil spill, sediment samples that were projected to have a high probability of being impacted by the oil were collected from shoreline zones of Texas, Louisiana, Mississippi, Alabama, and Florida. Sixty-one sites were sampled and analyzed for hydraulic conductivity, porosity, and grain-size distribution. The objective of this effort was to provide a set of baselAuthorsJohn T. Lisle, Norris N. ComerA survey of microbial community diversity in marine sediments impacted by petroleum hydrocarbons from the Gulf of Mexico and Atlantic shorelines, Texas to Florida
Microbial community genomic DNA was extracted from sediment samples collected along the Gulf of Mexico and Atlantic coasts from Texas to Florida. Sample sites were identified as being ecologically sensitive and (or) as having high potential of being impacted by Macondo-1 (M-1) well oil from the Deepwater Horizon blowout. The diversity within the microbial communities associated with the collectedAuthorsJohn T. Lisle, Sarah H. StellickA survey of alterations in microbial community diversity in marine sediments in response to oil from the Deepwater Horizon spill: Northern Gulf of Mexico shoreline, Texas to Florida
Microbial community genomic DNA was extracted from sediment samples collected from the northern Gulf of Mexico (NGOM) coast. These samples had a high probability of being impacted by Macondo-1 (M-1) well oil from the Deepwater Horizon (DWH) drilling site. The hypothesis for this project was that presence of M-1 oil in coastal sediments would significantly alter the diversity within the microbial cAuthorsJohn T. LisleWhen a habitat freezes solid: Microorganisms over-winter within the ice column of a coastal Antarctic lake
A major impediment to understanding the biology of microorganisms inhabiting Antarctic environments is the logistical constraint of conducting field work primarily during the summer season. However, organisms that persist throughout the year encounter severe environmental changes between seasons. In an attempt to bridge this gap, we collected ice core samples from Pony Lake in early November 2004AuthorsC.M. Foreman, M. Dieser, M. Greenwood, R.M. Cory, J. Laybourn-Parry, John T. Lisle, C. Jaros, P.L. Miller, Y.-P. Chin, Diane M. McKnight - News
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government