John Thomas Lisle

My research is focused on characterizing how microbes influence the geochemistry and carbon and nutrient cycling in surface water, ground water and coastal marine water and associated sediment systems thru the application of phylogenetics, microbial energetics and stable isotopes and radiolabeled substrates.


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1996    Ph.D., University of South Florida, College of Public Health

1983    M.S., Eastern Kentucky University, Department of Biology

1978    B.S., Eastern Kentucky University, Department of Biology


Professional Experience

2004-present   Microbial Ecologist, USGS Center for Coastal & Watershed Studies, St. Petersburg, FL

2002-present   Affiliate Graduate Faculty in the University of South Florida’s Biology Department

2002-2009       Assistant Courtesy Professor in the University of South Florida’s College of Marine Sciences

2002-2004       USGS Mendenhall Fellow, Center for Coastal & Watershed Studies, St. Petersburg, FL

2001-2002       Microbial Ecologist, NASA, Astrobiology Institute for the Study of Biomarkers, Johnson Space Center, Houston

2000-2001       Research Microbiologist, Lockheed Martin, NASA Astrobiology Institute for Biomarkers, Johnson Space Center, Houston

1998-2000       Assistant Research Professor, Department of Microbiology, Montana State University      

1996-1998       Post-doctoral research fellow at Montana State University/Dr. Gordon McFeters, Department of Microbiology


Additional Research Activities

Collaborating scientist (2005-2007). The influence on microbial activities on arsenic mobilization from Floridan aquifer material (Dr. John Arthur/FLDEP).  

Collaborating scientist (2005-2007). Microbial chemotaxis in hydrocarbon contaminated aquifers (Dr. Ron Harvey/USGS NRP).

Collaborating scientist (2004-Present). Characterization of bacterial and bacteriophage dynamics in ice and selected glacial melt streams and permanently ice-covered lakes in the Antarctic Dry Valleys (NSF LTER: (Dr. John Priscu/Montana State University) 


Lisle, J. and Robbins, L. (2016). Viral bursting of cyanobacteria as a mechanism for calcium carbonate nucleation and precipitation in seawater. Frontiers In Microbiology (10.3389/fmicb.2016.01958; In Press).

Lisle, J. (2016). Membrane diffusion chambers and above ground mesocosms for the study of microbial responses to groundwater geochemistry. Journal of Visualized Experiments (In Press).

Harvey, R., Underwood, J., Lisle, J., Metge, D., Aiken, G. (2014). Role of DOC in the fate and transport of Escherichia coli K12 in a deep limestone aquifer in South Florida. USGS Scientific Investigation Report 2014-5035, pp. 4. 

H. Browman, S. Dupont, J. Havenhand, L. Robbins,  M. Beman, C. Duarte, M. Fine, J. Fosså, J. Hall-Spencer, P. Hallock-Muller, T. Hurst, D. Iglesias-Rodriguez, P. Knorr, H. Kurihara, J. Lisle, C. Manno, S. McCoy, F. Melzner, P. Munday, H. Pörtner, J. Ries, D. Robert, J. Runge, D. Scott, H. Skjoldal, K. Suzuki, F. Thingstad, T. Wootton (2013). Biological responses to ocean acidification. Chp. 3, pp. 37-54. In: AMAP Assessment 2013: Arctic Ocean Acidification. Arctic Monitoring and Assessment Programme, Oslo, Norway. pp. 99.

Forman, C., Cory, R., Morris, C., SanClements, M., Smith, H., Lisle, J., Miller, P., Chin, Y. and McKnight, D. (2013). Microbial growth under humic-free conditions in a supraglacial stream system on the Cotton Glacier, Antarctica. Environmental Research Letters 8(3): 1-11. 

Dieser, M., Foreman, C., Jaros, C., Lisle, J., Greenwood, M., Laybourn-Parry, J., Miller, P., Chin, Y-. and McKnight, D. (2013). Physicochemical and biological dynamics in a coastal Antarctic lake as it transitions from frozen to open water. Antarctic Science 25(5): 663-675.

Foreman, C., Dieser, M., Greenwood, M., Cory, R., Laybourn-Parry, J., Lisle, J., Jaros, C., Miller, P., Chin, Y., McKnight, D. (2011). When a habitat freezes solid: microbes over-winter within the ice column of a coastal Antarctic lake.  FEMS Microbial Ecology, 76(3): 401-412.