Luke R Iwanowicz, Ph.D. (Former Employee)
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A method for determining avian influenza virus hemagglutinin and neuraminidase subtype association
Methods for grouping specific avian influenza virus (AIV) hemagglutinin (HA) and neuraminidase (NA) subtype reverse-transcription polymerase chain reaction (RT-PCR) products into HA:NA subtypes when egg incubation is technically not feasible were evaluated. These approaches were adopted for use as post hoc methods after melt curve analysis. The methods are based on ratios obtained from amplicon co
Authors
Christopher A. Ottinger, Deborah D. Iwanowicz, Luke R. Iwanowicz, Cynthia R. Adams, Lakyn R. Sanders, Christine L. Densmore
Temporal evaluation of estrogenic endocrine disruption markers in smallmouth bass (Micropterus dolomieu) reveals seasonal variability in intersex
A reconnaissance project completed in 2009 identified intersex and elevated plasma vitellogenin in male smallmouth bass inhabiting the Missisquoi River, VT. In an attempt to identify the presence and seasonality of putative endocrine disrupting chemicals or other factors associated with these observations, a comprehensive reevaluation was conducted between September 2012 and June 2014. Here, we co
Authors
Luke R. Iwanowicz, A.E. Pinkney, C.P. Guy, A.M. Major, K. Munney, Vicki S. Blazer, David Alvarez, Heather L. Walsh, Adam J. Sperry, Lakyn R. Sanders, D. R. Smith
An evaluation of the toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fish and their forage base
Potash, with the active ingredient potassium chloride (KCl) is a chemical that is currently being evaluated for potential use as a molluscicide to combat invasive zebra mussels and quagga mussels in Western United States waters. Although data available for other freshwater fishes indicate that recommended treatment levels of potash as a molluscicide are sublethal, this has not been demonstrated fo
Authors
Christine L. Densmore, Luke R. Iwanowicz, Anne P. Henderson, Vicki S. Blazer, Baileigh M. Reed-Grimmett, Lakyn R. Sanders
Pharmaceuticals, hormones, pesticides, and other bioactive contaminants in water, sediment, and tissue from Rocky Mountain National Park, 2012–2013
Pharmaceuticals, hormones, pesticides, and other bioactive contaminants (BCs) are commonly detected in surface water and bed sediment in urban and suburban areas, but these contaminants are understudied in remote locations. In Rocky Mountain National Park (RMNP), Colorado, USA, BCs may threaten the reproductive success and survival of native aquatic species, benthic communities, and pelagic food w
Authors
William A. Battaglin, Paul M. Bradley, Luke R. Iwanowicz, Celeste A. Journey, Heather L. Walsh, Vicki S. Blazer
Exposure to synthetic hydraulic fracturing waste influences the mucosal bacterial community structure of the brook trout (Salvelinus fontinalis) epidermis
Production of natural gas using unconventional technologies has risen as demand for alternative fuels has increased. Impacts on the environment from waste generated from these processes are largely unexplored. In particular, the outcomes of organismal exposure to hydraulic fracturing waste have not been rigorously evaluated. We evaluated the effects of exposure to surrogate hydraulic fracturing wa
Authors
Heather S. Galbraith, Deborah D. Iwanowicz, Daniel E. Spooner, Luke R. Iwanowicz, David Keller, Paula M. Zelanko, Cynthia R. Adams
Genomic evolution, recombination, and inter-strain diversity of chelonid alphaherpesvirus 5 from Florida and Hawaii green sea turtles with fibropapillomatosis
Chelonid alphaherpesvirus 5 (ChHV5) is a herpesvirus associated with fibropapillomatosis (FP) in sea turtles worldwide. Single-locus typing has previously shown differentiation between Atlantic and Pacific strains of this virus, with low variation within each geographic clade. However, a lack of multi-locus genomic sequence data hinders understanding of the rate and mechanisms of ChHV5 evolutionar
Authors
Cheryl L. Morrison, Luke R. Iwanowicz, Thierry M. Work, Elizabeth Fahsbender, Mya Breitbart, Cynthia R. Adams, Deborah D. Iwanowicz, Lakyn Sanders, Mathias Ackermann, Robert S. Cornman
Contaminant-associated health effects in fishes from the Ottawa and Ashtabula Rivers, Ohio
The health of resident fishes serves as a biologically relevant barometer of aquatic ecosystem integrity. Here, the health of the Ottawa River and Ashtabula River (both within the Lake Erie Basin) were assessed using morphological and immunological biomarkers in brown bullheads (Ameiurus nebulosus) and largemouth bass (Micropterus salmoides). Biomarker metrics were compared to fish collected from
Authors
Luke R. Iwanowicz, Vicki S. Blazer, Heather L. Walsh, Cassidy H. Shaw, David S. DeVault, Jo A. Banda
Investigating the influence of nitrate nitrogen on post-smolt Atlantic salmon Salmo salar reproductive physiology in water recirculation aquaculture systems
A major issue affecting land-based, closed containment Atlantic salmon Salmo salar growout production in water recirculation aquaculture systems (RAS) is precocious male maturation, which can negatively impact factors such as feed conversion, fillet yield, and product quality. Along with other water quality parameters, elevated nitrate nitrogen (NO3-N) has been shown to influence the reproductive
Authors
Christopher Good, John Davidson, Luke R. Iwanowicz, Michael T. Meyer, Julie E. Dietze, Dana W. Kolpin, David Marancik, Jill Birkett, Christina Williams, Steven T. Summerfelt
Draft genome sequence of a picorna-like virus associated with gill tissue in clinically normal brook trout, Salvelinus fontinalis
Here, we report a draft genome sequence of a picorna-like virus associated with brook trout, Salvelinus fontinalis, gill tissue. The draft genome comprises 8,681 nucleotides, excluding the poly(A) tract, and contains two open reading frames. It is most similar to picorna-like viruses that infect invertebrates.
Authors
Luke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Heather S. Galbraith, Aaron Aunins, Robert S. Cornman
Highlighting the complexities of a groundwater pilot study during an avian influenza outbreak: Methods, lessons learned, and select contaminant results
The highly pathogenic avian influenza (H5N2) outbreak in the Midwestern United States (US) in 2015 was historic due to the number of birds and poultry operations impacted and the corresponding economic loss to the poultry industry and was the largest animal health emergency in US history. The U.S. Geological Survey (USGS), with the assistance of several state and federal agencies, aided the respon
Authors
Laura E. Hubbard, Dana W. Kolpin, Chad L. Fields, Michelle L. Hladik, Luke R. Iwanowicz
Widespread occurrence and potential for biodegradation of bioactive contaminants in Congaree National Park, USA
Organic contaminants with designed molecular bioactivity, such as pesticides and pharmaceuticals, originate from human and agricultural sources, occur frequently in surface waters, and threaten the structure and function of aquatic and terrestrial ecosystems. Congaree National Park in South Carolina (USA) is a vulnerable park unit due to its location downstream of multiple urban and agricultural c
Authors
Paul M. Bradley, William A. Battaglin, Jimmy M. Clark, Frank Henning, Michelle L. Hladik, Luke R. Iwanowicz, Celeste A. Journey, Jeffrey W. Riley, Kristin M. Romanok
Occurrence and in vitro bioactivity of estrogen, androgen, and glucocorticoid compounds in a nationwide screen of United States stream waters
In vitro bioassays are sensitive, effect-based tools used to quantitatively screen for chemicals with nuclear receptor activity in environmental samples. We measured in vitro estrogen (ER), androgen (AR), and glucocorticoid receptor (GR) activity, along with a broad suite of chemical analytes, in streamwater from 35 well-characterized sites (3 reference and 32 impacted) across 24 states and Puerto
Authors
Justin M. Conley, Nicola Evans, Mary C. Cardon, Laura Rosenblum, Luke R. Iwanowicz, Phillip C. Hartig, Kathleen M. Schenck, Paul M. Bradley, Vickie S. Wilson
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
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A method for determining avian influenza virus hemagglutinin and neuraminidase subtype association
Methods for grouping specific avian influenza virus (AIV) hemagglutinin (HA) and neuraminidase (NA) subtype reverse-transcription polymerase chain reaction (RT-PCR) products into HA:NA subtypes when egg incubation is technically not feasible were evaluated. These approaches were adopted for use as post hoc methods after melt curve analysis. The methods are based on ratios obtained from amplicon coAuthorsChristopher A. Ottinger, Deborah D. Iwanowicz, Luke R. Iwanowicz, Cynthia R. Adams, Lakyn R. Sanders, Christine L. DensmoreTemporal evaluation of estrogenic endocrine disruption markers in smallmouth bass (Micropterus dolomieu) reveals seasonal variability in intersex
A reconnaissance project completed in 2009 identified intersex and elevated plasma vitellogenin in male smallmouth bass inhabiting the Missisquoi River, VT. In an attempt to identify the presence and seasonality of putative endocrine disrupting chemicals or other factors associated with these observations, a comprehensive reevaluation was conducted between September 2012 and June 2014. Here, we coAuthorsLuke R. Iwanowicz, A.E. Pinkney, C.P. Guy, A.M. Major, K. Munney, Vicki S. Blazer, David Alvarez, Heather L. Walsh, Adam J. Sperry, Lakyn R. Sanders, D. R. SmithAn evaluation of the toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fish and their forage base
Potash, with the active ingredient potassium chloride (KCl) is a chemical that is currently being evaluated for potential use as a molluscicide to combat invasive zebra mussels and quagga mussels in Western United States waters. Although data available for other freshwater fishes indicate that recommended treatment levels of potash as a molluscicide are sublethal, this has not been demonstrated foAuthorsChristine L. Densmore, Luke R. Iwanowicz, Anne P. Henderson, Vicki S. Blazer, Baileigh M. Reed-Grimmett, Lakyn R. SandersPharmaceuticals, hormones, pesticides, and other bioactive contaminants in water, sediment, and tissue from Rocky Mountain National Park, 2012–2013
Pharmaceuticals, hormones, pesticides, and other bioactive contaminants (BCs) are commonly detected in surface water and bed sediment in urban and suburban areas, but these contaminants are understudied in remote locations. In Rocky Mountain National Park (RMNP), Colorado, USA, BCs may threaten the reproductive success and survival of native aquatic species, benthic communities, and pelagic food wAuthorsWilliam A. Battaglin, Paul M. Bradley, Luke R. Iwanowicz, Celeste A. Journey, Heather L. Walsh, Vicki S. BlazerExposure to synthetic hydraulic fracturing waste influences the mucosal bacterial community structure of the brook trout (Salvelinus fontinalis) epidermis
Production of natural gas using unconventional technologies has risen as demand for alternative fuels has increased. Impacts on the environment from waste generated from these processes are largely unexplored. In particular, the outcomes of organismal exposure to hydraulic fracturing waste have not been rigorously evaluated. We evaluated the effects of exposure to surrogate hydraulic fracturing waAuthorsHeather S. Galbraith, Deborah D. Iwanowicz, Daniel E. Spooner, Luke R. Iwanowicz, David Keller, Paula M. Zelanko, Cynthia R. AdamsGenomic evolution, recombination, and inter-strain diversity of chelonid alphaherpesvirus 5 from Florida and Hawaii green sea turtles with fibropapillomatosis
Chelonid alphaherpesvirus 5 (ChHV5) is a herpesvirus associated with fibropapillomatosis (FP) in sea turtles worldwide. Single-locus typing has previously shown differentiation between Atlantic and Pacific strains of this virus, with low variation within each geographic clade. However, a lack of multi-locus genomic sequence data hinders understanding of the rate and mechanisms of ChHV5 evolutionarAuthorsCheryl L. Morrison, Luke R. Iwanowicz, Thierry M. Work, Elizabeth Fahsbender, Mya Breitbart, Cynthia R. Adams, Deborah D. Iwanowicz, Lakyn Sanders, Mathias Ackermann, Robert S. CornmanContaminant-associated health effects in fishes from the Ottawa and Ashtabula Rivers, Ohio
The health of resident fishes serves as a biologically relevant barometer of aquatic ecosystem integrity. Here, the health of the Ottawa River and Ashtabula River (both within the Lake Erie Basin) were assessed using morphological and immunological biomarkers in brown bullheads (Ameiurus nebulosus) and largemouth bass (Micropterus salmoides). Biomarker metrics were compared to fish collected fromAuthorsLuke R. Iwanowicz, Vicki S. Blazer, Heather L. Walsh, Cassidy H. Shaw, David S. DeVault, Jo A. BandaInvestigating the influence of nitrate nitrogen on post-smolt Atlantic salmon Salmo salar reproductive physiology in water recirculation aquaculture systems
A major issue affecting land-based, closed containment Atlantic salmon Salmo salar growout production in water recirculation aquaculture systems (RAS) is precocious male maturation, which can negatively impact factors such as feed conversion, fillet yield, and product quality. Along with other water quality parameters, elevated nitrate nitrogen (NO3-N) has been shown to influence the reproductiveAuthorsChristopher Good, John Davidson, Luke R. Iwanowicz, Michael T. Meyer, Julie E. Dietze, Dana W. Kolpin, David Marancik, Jill Birkett, Christina Williams, Steven T. SummerfeltDraft genome sequence of a picorna-like virus associated with gill tissue in clinically normal brook trout, Salvelinus fontinalis
Here, we report a draft genome sequence of a picorna-like virus associated with brook trout, Salvelinus fontinalis, gill tissue. The draft genome comprises 8,681 nucleotides, excluding the poly(A) tract, and contains two open reading frames. It is most similar to picorna-like viruses that infect invertebrates.AuthorsLuke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Heather S. Galbraith, Aaron Aunins, Robert S. CornmanHighlighting the complexities of a groundwater pilot study during an avian influenza outbreak: Methods, lessons learned, and select contaminant results
The highly pathogenic avian influenza (H5N2) outbreak in the Midwestern United States (US) in 2015 was historic due to the number of birds and poultry operations impacted and the corresponding economic loss to the poultry industry and was the largest animal health emergency in US history. The U.S. Geological Survey (USGS), with the assistance of several state and federal agencies, aided the responAuthorsLaura E. Hubbard, Dana W. Kolpin, Chad L. Fields, Michelle L. Hladik, Luke R. IwanowiczWidespread occurrence and potential for biodegradation of bioactive contaminants in Congaree National Park, USA
Organic contaminants with designed molecular bioactivity, such as pesticides and pharmaceuticals, originate from human and agricultural sources, occur frequently in surface waters, and threaten the structure and function of aquatic and terrestrial ecosystems. Congaree National Park in South Carolina (USA) is a vulnerable park unit due to its location downstream of multiple urban and agricultural cAuthorsPaul M. Bradley, William A. Battaglin, Jimmy M. Clark, Frank Henning, Michelle L. Hladik, Luke R. Iwanowicz, Celeste A. Journey, Jeffrey W. Riley, Kristin M. RomanokOccurrence and in vitro bioactivity of estrogen, androgen, and glucocorticoid compounds in a nationwide screen of United States stream waters
In vitro bioassays are sensitive, effect-based tools used to quantitatively screen for chemicals with nuclear receptor activity in environmental samples. We measured in vitro estrogen (ER), androgen (AR), and glucocorticoid receptor (GR) activity, along with a broad suite of chemical analytes, in streamwater from 35 well-characterized sites (3 reference and 32 impacted) across 24 states and PuertoAuthorsJustin M. Conley, Nicola Evans, Mary C. Cardon, Laura Rosenblum, Luke R. Iwanowicz, Phillip C. Hartig, Kathleen M. Schenck, Paul M. Bradley, Vickie S. WilsonNon-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
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*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government