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Protocols for the Detection of Renibacterium salmoninarum in salmon
Renibacterium salmoninarum is a fastidious, gram-positive bacterium and the causative agent of bacterial kidney disease (BKD) in wild and cultured salmonid fishes. A number of diagnostic tests exist for the direct and indirect detection of R. salmoninarum. These tests have different analytical properties and different abilities to quantify infection levels, detect remote infections and distinguish between live and dead bacteria. This website links to standard operating procedures (SOP) for the major R. salmoninarum diagnostic tests.
Quantitative Culture for R. salmoninarum - (2011) (Culture of Renibacterium salmoninarum from Tissues and Ovarian Fluid)
Enzyme linked immunosorbent assay (ELISA) for R. salmoninarum – (2012) (Double Polyclonal Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of Renibacterium salmoninarum)
Direct fluorescent antibody test (DFAT) – (2011) (Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues)
Membrane filtration fluorescent antibody test (MF-FAT) – (2012) (Membrane-Filtration Fluorescent Antibody Test (MF-FAT) for the detection of Renibacterium salmoninarum)
DNA extraction for gram positive bacteria – (2011) (DNA Extraction for PCR-based detection of Renibacterium salmoninarum)
Nested PCR (nPCR) test – (2011) (Nested Polymerase Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum)
Quantitative PCR (qPCR) test – (Quantitative Polymerase Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum)
Below are multimedia items associated with this project.
Title: Culture of Renibacterium salmoninarum from Tissues and Ovarian Fluid
Number: BACT-1
Version: 01 Created April 19, 2011
Purpose: Culture of Renibacterium salmoninarum in fish tissues or ovarian fluids
Title: Culture of Renibacterium salmoninarum from Tissues and Ovarian Fluid
Number: BACT-1
Version: 01 Created April 19, 2011
Purpose: Culture of Renibacterium salmoninarum in fish tissues or ovarian fluids
Title: Double polyclonal enzyme-linked immunosorbent assay (ELISA) for the detection of Renibacterium salmonarium
Number: BACT-2
Version: 02
Purpose: To detect Renibacterium salmoninarum soluble antigen in fish tissues or fluids
Title: Double polyclonal enzyme-linked immunosorbent assay (ELISA) for the detection of Renibacterium salmonarium
Number: BACT-2
Version: 02
Purpose: To detect Renibacterium salmoninarum soluble antigen in fish tissues or fluids
Title: Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues
Number: BACT-3
Version: 02 Created December 14, 2011
Purpose: Detection of Renibacterium salmoninarum cells in fish tissues or culture
Title: Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues
Number: BACT-3
Version: 02 Created December 14, 2011
Purpose: Detection of Renibacterium salmoninarum cells in fish tissues or culture
Title: Membrane-Filtration Fluorescent Antibody Text (MF-FAT) for the detection of Renibacterium salmoninarum
Number: BACT-4
Version: 02 Created January 10, 2012
Purpose: Detection of Renibacterium salmoninarum in fish fluids or broth culture
Title: Membrane-Filtration Fluorescent Antibody Text (MF-FAT) for the detection of Renibacterium salmoninarum
Number: BACT-4
Version: 02 Created January 10, 2012
Purpose: Detection of Renibacterium salmoninarum in fish fluids or broth culture
Title: DNA Extraction for PCR-based Detection of Renibacterium salmoninarum
Number: BACT-5
Version: 01 Created April 15, 2011
Purpose: Recommended DNA extraction procedure of fish tissues and ovarian fluids for PCR-based detection of Renibacterium salmoninarum
Title: DNA Extraction for PCR-based Detection of Renibacterium salmoninarum
Number: BACT-5
Version: 01 Created April 15, 2011
Purpose: Recommended DNA extraction procedure of fish tissues and ovarian fluids for PCR-based detection of Renibacterium salmoninarum
Title: Nested Polymerase Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum
Number: BACT-6
Version: 01 Created April 15, 2011
Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using a nested PCR primer set
Title: Nested Polymerase Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum
Number: BACT-6
Version: 01 Created April 15, 2011
Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using a nested PCR primer set
Title: Quantitative Polymerase Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum
Number: BACT-7
Version: 02 Created December 14, 2011
Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using quantitative PCR
Title: Quantitative Polymerase Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum
Number: BACT-7
Version: 02 Created December 14, 2011
Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using quantitative PCR
Protocols for the Detection of Renibacterium salmoninarum in salmon
Renibacterium salmoninarum is a fastidious, gram-positive bacterium and the causative agent of bacterial kidney disease (BKD) in wild and cultured salmonid fishes. A number of diagnostic tests exist for the direct and indirect detection of R. salmoninarum. These tests have different analytical properties and different abilities to quantify infection levels, detect remote infections and distinguish between live and dead bacteria. This website links to standard operating procedures (SOP) for the major R. salmoninarum diagnostic tests.
Quantitative Culture for R. salmoninarum - (2011) (Culture of Renibacterium salmoninarum from Tissues and Ovarian Fluid)
Enzyme linked immunosorbent assay (ELISA) for R. salmoninarum – (2012) (Double Polyclonal Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of Renibacterium salmoninarum)
Direct fluorescent antibody test (DFAT) – (2011) (Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues)
Membrane filtration fluorescent antibody test (MF-FAT) – (2012) (Membrane-Filtration Fluorescent Antibody Test (MF-FAT) for the detection of Renibacterium salmoninarum)
DNA extraction for gram positive bacteria – (2011) (DNA Extraction for PCR-based detection of Renibacterium salmoninarum)
Nested PCR (nPCR) test – (2011) (Nested Polymerase Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum)
Quantitative PCR (qPCR) test – (Quantitative Polymerase Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum)
Below are multimedia items associated with this project.
Title: Culture of Renibacterium salmoninarum from Tissues and Ovarian Fluid
Number: BACT-1
Version: 01 Created April 19, 2011
Purpose: Culture of Renibacterium salmoninarum in fish tissues or ovarian fluids
Title: Culture of Renibacterium salmoninarum from Tissues and Ovarian Fluid
Number: BACT-1
Version: 01 Created April 19, 2011
Purpose: Culture of Renibacterium salmoninarum in fish tissues or ovarian fluids
Title: Double polyclonal enzyme-linked immunosorbent assay (ELISA) for the detection of Renibacterium salmonarium
Number: BACT-2
Version: 02
Purpose: To detect Renibacterium salmoninarum soluble antigen in fish tissues or fluids
Title: Double polyclonal enzyme-linked immunosorbent assay (ELISA) for the detection of Renibacterium salmonarium
Number: BACT-2
Version: 02
Purpose: To detect Renibacterium salmoninarum soluble antigen in fish tissues or fluids
Title: Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues
Number: BACT-3
Version: 02 Created December 14, 2011
Purpose: Detection of Renibacterium salmoninarum cells in fish tissues or culture
Title: Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues
Number: BACT-3
Version: 02 Created December 14, 2011
Purpose: Detection of Renibacterium salmoninarum cells in fish tissues or culture
Title: Membrane-Filtration Fluorescent Antibody Text (MF-FAT) for the detection of Renibacterium salmoninarum
Number: BACT-4
Version: 02 Created January 10, 2012
Purpose: Detection of Renibacterium salmoninarum in fish fluids or broth culture
Title: Membrane-Filtration Fluorescent Antibody Text (MF-FAT) for the detection of Renibacterium salmoninarum
Number: BACT-4
Version: 02 Created January 10, 2012
Purpose: Detection of Renibacterium salmoninarum in fish fluids or broth culture
Title: DNA Extraction for PCR-based Detection of Renibacterium salmoninarum
Number: BACT-5
Version: 01 Created April 15, 2011
Purpose: Recommended DNA extraction procedure of fish tissues and ovarian fluids for PCR-based detection of Renibacterium salmoninarum
Title: DNA Extraction for PCR-based Detection of Renibacterium salmoninarum
Number: BACT-5
Version: 01 Created April 15, 2011
Purpose: Recommended DNA extraction procedure of fish tissues and ovarian fluids for PCR-based detection of Renibacterium salmoninarum
Title: Nested Polymerase Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum
Number: BACT-6
Version: 01 Created April 15, 2011
Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using a nested PCR primer set
Title: Nested Polymerase Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum
Number: BACT-6
Version: 01 Created April 15, 2011
Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using a nested PCR primer set
Title: Quantitative Polymerase Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum
Number: BACT-7
Version: 02 Created December 14, 2011
Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using quantitative PCR
Title: Quantitative Polymerase Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum
Number: BACT-7
Version: 02 Created December 14, 2011
Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using quantitative PCR