Picture of Cape Alava at sunset. Cape Alava is a point of land on the outer coast of the Olympic Peninsula. The cape is situated within Olympic National Park, and the Makah Indian Reservation.
Carla M Conway
Carla is a Biological Science Laboratory Technician (Microbiology) at the Western Fisheries Research Center.
Science and Products
Non-lethal Detection of Skin Injuries in Juvenile Chinook Salmon Oncorhynchus tshawytscha by Fast Green FCF Dye
In fish, as in humans, an intact epidermis is critical to defense against entry of pathogens into the skin. Macroscopic examination of scale loss is the principal method of evaluating physical damage to juvenile salmonids out-migrating through hydroelectric dams in the Snake and Columbia Rivers, and in fish subjected to capture and handling procedures in locations such as hatcheries, fish bypass...
Cape Alava sunset
Picture of Cape Alava at sunset. Cape Alava is a point of land on the outer coast of the Olympic Peninsula. The cape is situated within Olympic National Park, and the Makah Indian Reservation.
Gills from Lost River Suckers - heavy infestation of Ichthyobodo sp.
Gills from Lost River suckers with a heavy infestation of Ichthyobodo sp. (arrows). Slide is stained with hematoxylin and eosin.
Gills from Lost River suckers with a heavy infestation of Ichthyobodo sp. (arrows). Slide is stained with hematoxylin and eosin.
WFRC DFAT for detection of Renibacterium salmoninarum
Direct fluorescent antibody test (DFAT) for the detection of Renibacterium salmoninarum in tissues. Fluorescing R. salmoninarum cells are visible on a slide.
Direct fluorescent antibody test (DFAT) for the detection of Renibacterium salmoninarum in tissues. Fluorescing R. salmoninarum cells are visible on a slide.
Figure 1. Appearance of Descaling Site Exposed to Fast Green FCF Dye
Figure 1. Appearance of descaling site exposed to fast green FCF dye six hours after intentional descaling injury, showing loss of scales and presence of fast green staining. Areas of unintentional integumental injury are also stained (arrows).
Related image Figure 2.
Figure 1. Appearance of descaling site exposed to fast green FCF dye six hours after intentional descaling injury, showing loss of scales and presence of fast green staining. Areas of unintentional integumental injury are also stained (arrows).
Related image Figure 2.
Figure 2. Scanning Electron Micrograph of Descaling Area
Figure 2. Scanning electron micrograph of descaling area delimited by box in Figure 1 showing epidermal disruption, empty scale pockets and an exposed scale with visible concentric ridges (upper right). Scale bar = 500 µm.
Figure 2. Scanning electron micrograph of descaling area delimited by box in Figure 1 showing epidermal disruption, empty scale pockets and an exposed scale with visible concentric ridges (upper right). Scale bar = 500 µm.
Figure 3. Appearance of Descaling Site Exposed to Fast Green FCF Dye
Figure 3. Appearance of descaling site exposed to fast green FCF dye 96 hours after intentional descaling injury, showing lack of scales, presence of fast green staining in areas of epidermal disruption and absence of staining in areas where migrating epidermal cells have closed the wound.
Figure 3. Appearance of descaling site exposed to fast green FCF dye 96 hours after intentional descaling injury, showing lack of scales, presence of fast green staining in areas of epidermal disruption and absence of staining in areas where migrating epidermal cells have closed the wound.
Figure 4. Scanning Electron Micrograph of Descaling Area
Figure 4. Scanning electron micrograph of descaling area delimited by box in Figure 3 showing epidermal disruption (arrows), empty scale pockets and restoration of epidermal integrity (asterisk). An exposed scale with visible concentric ridges is visible at the lower center. Scale bar = 500 µm.
Figure 4. Scanning electron micrograph of descaling area delimited by box in Figure 3 showing epidermal disruption (arrows), empty scale pockets and restoration of epidermal integrity (asterisk). An exposed scale with visible concentric ridges is visible at the lower center. Scale bar = 500 µm.
Science and Products
Non-lethal Detection of Skin Injuries in Juvenile Chinook Salmon Oncorhynchus tshawytscha by Fast Green FCF Dye
In fish, as in humans, an intact epidermis is critical to defense against entry of pathogens into the skin. Macroscopic examination of scale loss is the principal method of evaluating physical damage to juvenile salmonids out-migrating through hydroelectric dams in the Snake and Columbia Rivers, and in fish subjected to capture and handling procedures in locations such as hatcheries, fish bypass...
Cape Alava sunset
Picture of Cape Alava at sunset. Cape Alava is a point of land on the outer coast of the Olympic Peninsula. The cape is situated within Olympic National Park, and the Makah Indian Reservation.
Picture of Cape Alava at sunset. Cape Alava is a point of land on the outer coast of the Olympic Peninsula. The cape is situated within Olympic National Park, and the Makah Indian Reservation.
Gills from Lost River Suckers - heavy infestation of Ichthyobodo sp.
Gills from Lost River suckers with a heavy infestation of Ichthyobodo sp. (arrows). Slide is stained with hematoxylin and eosin.
Gills from Lost River suckers with a heavy infestation of Ichthyobodo sp. (arrows). Slide is stained with hematoxylin and eosin.
WFRC DFAT for detection of Renibacterium salmoninarum
Direct fluorescent antibody test (DFAT) for the detection of Renibacterium salmoninarum in tissues. Fluorescing R. salmoninarum cells are visible on a slide.
Direct fluorescent antibody test (DFAT) for the detection of Renibacterium salmoninarum in tissues. Fluorescing R. salmoninarum cells are visible on a slide.
Figure 1. Appearance of Descaling Site Exposed to Fast Green FCF Dye
Figure 1. Appearance of descaling site exposed to fast green FCF dye six hours after intentional descaling injury, showing loss of scales and presence of fast green staining. Areas of unintentional integumental injury are also stained (arrows).
Related image Figure 2.
Figure 1. Appearance of descaling site exposed to fast green FCF dye six hours after intentional descaling injury, showing loss of scales and presence of fast green staining. Areas of unintentional integumental injury are also stained (arrows).
Related image Figure 2.
Figure 2. Scanning Electron Micrograph of Descaling Area
Figure 2. Scanning electron micrograph of descaling area delimited by box in Figure 1 showing epidermal disruption, empty scale pockets and an exposed scale with visible concentric ridges (upper right). Scale bar = 500 µm.
Figure 2. Scanning electron micrograph of descaling area delimited by box in Figure 1 showing epidermal disruption, empty scale pockets and an exposed scale with visible concentric ridges (upper right). Scale bar = 500 µm.
Figure 3. Appearance of Descaling Site Exposed to Fast Green FCF Dye
Figure 3. Appearance of descaling site exposed to fast green FCF dye 96 hours after intentional descaling injury, showing lack of scales, presence of fast green staining in areas of epidermal disruption and absence of staining in areas where migrating epidermal cells have closed the wound.
Figure 3. Appearance of descaling site exposed to fast green FCF dye 96 hours after intentional descaling injury, showing lack of scales, presence of fast green staining in areas of epidermal disruption and absence of staining in areas where migrating epidermal cells have closed the wound.
Figure 4. Scanning Electron Micrograph of Descaling Area
Figure 4. Scanning electron micrograph of descaling area delimited by box in Figure 3 showing epidermal disruption (arrows), empty scale pockets and restoration of epidermal integrity (asterisk). An exposed scale with visible concentric ridges is visible at the lower center. Scale bar = 500 µm.
Figure 4. Scanning electron micrograph of descaling area delimited by box in Figure 3 showing epidermal disruption (arrows), empty scale pockets and restoration of epidermal integrity (asterisk). An exposed scale with visible concentric ridges is visible at the lower center. Scale bar = 500 µm.