Detection of Dakota skipper eDNA from inflorescences in the Upper Midwest, June and July 2022 (ver. 1.1, November 2025)
September 5, 2025
This dataset contains environmental DNA (eDNA) data for two separate tests of a novel quantitative polymerase chain reaction (qPCR) assay for detection of Dakota skipper (Hesperia dacotae) from inflorescences. One test was conducted in a controlled setting, and the other was conducted in a field setting. Also included are qPCR data for negative controls and standard dilutions of Dakota skipper gBlock.
Citation Information
| Publication Year | 2025 |
|---|---|
| Title | Detection of Dakota skipper eDNA from inflorescences in the Upper Midwest, June and July 2022 (ver. 1.1, November 2025) |
| DOI | 10.5066/P13JBGIU |
| Authors | David Pilliod, Michaela R Grossklaus, Stacie A Kageyama, Cale Nordmeyer, Jerry Reinisch, Erik Runquist, Stephen F Spear |
| Product Type | Data Release |
| Record Source | USGS Asset Identifier Service (AIS) |
| USGS Organization | Forest and Rangeland Ecosystem Science Center (FRESC) Headquarters |
| Rights | This work is marked with CC0 1.0 Universal |
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A 21st Century butterfly net: Using eDNA to detect the imperiled Dakota skipper A 21st Century butterfly net: Using eDNA to detect the imperiled Dakota skipper
The development of environmental DNA (eDNA) methods for terrestrial arthropods could be transformative for the difficult task of assessing the status of species of conservation concern. The primary goal of this study was to investigate the efficacy of detecting the Dakota skipper (Hesperia dacotae) from its DNA left behind on inflorescences as a means of inferring species presence. We...
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A 21st Century butterfly net: Using eDNA to detect the imperiled Dakota skipper A 21st Century butterfly net: Using eDNA to detect the imperiled Dakota skipper
The development of environmental DNA (eDNA) methods for terrestrial arthropods could be transformative for the difficult task of assessing the status of species of conservation concern. The primary goal of this study was to investigate the efficacy of detecting the Dakota skipper (Hesperia dacotae) from its DNA left behind on inflorescences as a means of inferring species presence. We...
Authors
David Pilliod, Michaela Grossklaus, Stacie Kageyama, Cale Nordmeyer, Jerry Reinisch, Erik Runquist, Stephen Spear
Stephen Spear, PhD
Research Biologist
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Phone