This data release supports the manuscript: Walker, R.H. and A.W. Walters. 2019. A mechanistic understanding of ecological responses to anthropogenic surface disturbance in headwater streams. Ecosphere 10(10):e02907. 10.1002/ecs2.2907. It includes one data set with physicochemical and biological data from 40 stream sites (100 m length) in the Wyoming Range for summer 2016.