Lophelia Metagenome Extraction Protocol - Step 5

Video showing Step 5 of the  Lophelia Metagenome Extraction Protocol. 

5. Cell Capture
a. Filter the ~7 ml of the Top extraction through a Sterivex 0.22-µm filter to concentrate microbes on the filter. This is done by attaching a sterile 10 ml syringe to the top of the Sterivex filter, pouring the 7 ml sample into the syringe and then inserting the plunger to force the liquid through the filter and into the 15 ml tube below.
b. Detach the syringe from the filter, remove the plunger, refill with the filtrate, insert plunger, filter. Repeat one more time (for a total of three passes of the liquid through the Sterivex filter).
c. The liquid can now be discarded. The microbes are on the filter.