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A first application of enzyme-linked immunosorbent assay for screening cyclodiene insecticides in ground water

January 1, 1996

A commercially available enzyme-linked immunosorbent assay (ELISA) plate kit for screening of cyclodiene insecticides (aldrin, chlordane, dieldrin, endosulfan, endrin, and heptachlor) was evaluated for sensitivity, cross reactivity, and overall performance using ground-water samples from a contaminated site. Ground-water contaminants included several pesticide compounds and their manufacturing by-products, as well as many other organic and inorganic compounds. Cross-reactivity studies were carried out for the cyclodiene compounds, and results were compared to those listed by the manufacturer. Data obtained were used to evaluate the sensitivity of the ELISA kit to the cyclodiene compounds in ground water samples with a contaminated matrix. The method quantitation limit for the ELISA kit was 15 μg/L (as chlordane). Of the 56 ground-water samples analyzed using the ELISA plate kits, more than 85% showed cyclodiene insecticide contamination. The ELISA kit showed excellent potential as a screening tool for sites with suspected ground-water contamination by insecticides.

Publication Year 1996
Title A first application of enzyme-linked immunosorbent assay for screening cyclodiene insecticides in ground water
DOI 10.1021/bk-1996-0646.ch013
Authors T. R. Dombrowski, E. M. Thurman, G. B. Mohrman
Publication Type Article
Publication Subtype Journal Article
Series Title ACS Symposium Series
Index ID 70018071
Record Source USGS Publications Warehouse