A mixture of closely related streptomyces fermentation products, antimycin A, Is separated, and the components are identified by using reversed-phase high-performance liquid chromatography with directly linked 400-MHz proton nuclear magnetic resonance detection. Analyses of mixtures of three amino acids, alanine, glycine, and valine, are used to determine optimal measurement conditions. Sensitivity increases of as much as a factor of 3 are achieved, at the expense of some loss in chromatographic resolution, by use of an 80-μL NMR cell, Instead of a smaller 14-μL cell. Analysis of the antimycin A mixture, using the optimal analytical high performance liquid chromatography/nuclear magnetic resonance conditions, reveals it to consist of at least 10 closely related components.
|Title||Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic-resonance spectrometry|
|Authors||Steven T. K. Ha, Charles L. Wilkins, Sharon L. Abidi|
|Publication Subtype||Journal Article|
|Series Title||Analytical Chemistry|
|Record Source||USGS Publications Warehouse|
|USGS Organization||Upper Midwest Environmental Sciences Center|