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Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic-resonance spectrometry

March 1, 1989

A mixture of closely related streptomyces fermentation products, antimycin A, Is separated, and the components are identified by using reversed-phase high-performance liquid chromatography with directly linked 400-MHz proton nuclear magnetic resonance detection. Analyses of mixtures of three amino acids, alanine, glycine, and valine, are used to determine optimal measurement conditions. Sensitivity increases of as much as a factor of 3 are achieved, at the expense of some loss in chromatographic resolution, by use of an 80-μL NMR cell, Instead of a smaller 14-μL cell. Analysis of the antimycin A mixture, using the optimal analytical high performance liquid chromatography/nuclear magnetic resonance conditions, reveals it to consist of at least 10 closely related components.

Publication Year 1989
Title Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic-resonance spectrometry
DOI 10.1021/ac00180a005
Authors Steven T. K. Ha, Charles L. Wilkins, Sharon L. Abidi
Publication Type Article
Publication Subtype Journal Article
Series Title Analytical Chemistry
Index ID 70006923
Record Source USGS Publications Warehouse
USGS Organization Upper Midwest Environmental Sciences Center
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