Appendix C: Interim report on subtask focused on resampling historic Kennedy/ITD plots for RP-284

In October 2019, an Idaho Transportation Department (ITD) Cooperative Transportation Research Program award was made to Boise State University in partnership with the U.S. Geological Survey to investigate the use of weed-suppressive bacteria (Pseudomonas fluorescens strain ACK55) with preemergent herbicides (imazapic and indaziflam) to reduce exotic annual grasses (cheatgrass, medusahead) on ITD right-of-ways. The work includes a subtask in which ITD right-of-ways treated with ACK55 by Dr. Ann Kennedy 4-5 years previously (2017 report; ITD-RP-258) were resampled in summer 2020, focusing only on ACK55 and not the herbicides (which are tested separately and will be reported on in the future). The 2020 sampling protocol was similar but more intensive than the ITD-RP-258. There were no differences in annual grasses on areas sprayed with ACK55 and nearby untreated areas.