Assimilation efficiency of rainbow trout fed natural diets

Assimilation efficiency is a critical assumption of stable isotope mixing models and bioenergetics models, yet few studies examine how assimilation efficiency influences modeling inferences. We conducted laboratory experiments to determine rainbow trout (Oncorhynchus mykiss) assimilation efficiencies. Assimilation efficiency averaged 55.8% (SE ± 0.90) and 64.5% (SE ± 1.98) at the 10% and 25% ration levels, respectively, and did not differ significantly. Caloric energy egested in feces was not significantly different between ration levels. Caloric energy excreted in ammonia and urea was significantly different between the ration levels, with a higher amount at the 25% ration level. Absorption efficiency was significantly greater at a higher ration level. Percent energy egested in feces was significantly greater at lower ration levels. Percent energy excreted as ammonia and urea was high compared to previous studies and did not differ significantly by ration level. Our estimates of assimilation efficiency of rainbow trout (56% and 65%) were lower than some previously reported estimates, and our estimates of energy losses (feces and ammonia) were higher than some previous estimates. As knowledge of species-specific assimilation efficiencies increases, our ability to draw strong inferences and improve the accuracy of model predictions will improve.