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Biofilm formation of Francisella noatunensis subsp. orientalis

November 19, 2015

Francisella noatunensis subsp. orientalis (Fno) is an emergent fish pathogen in both marine and fresh water environments. The bacterium is suspected to persist in the environment even without the presence of a suitable fish host. In the present study, the influence of different abiotic factors such as salinity and temperature were used to study the biofilm formation of different isolates of Fno including intracellular growth loci C (iglC)and pathogenicity determinant protein A (pdpA) knockout strains. Finally, we compared the susceptibility of planktonic and biofilm to three disinfectants used in the aquaculture and ornamental fish industry, namely Virkon®, bleach and hydrogen peroxide. The data indicates that Fno is capable of producing biofilms within 24 h where both salinity as well as temperature plays a role in the growth and biofilm formation of Fno. Mutations in theiglC or pdpA, both known virulence factors, do not appear to affect the capacity of Fno to produce biofilms, and the minimum inhibitory concentration, and minimum biocidal concentration for the three disinfectants were lower than the minimum biofilm eradication concentration values. This information needs to be taken into account if trying to eradicate the pathogen from aquaculture facilities or aquariums.

Publication Year 2015
Title Biofilm formation of Francisella noatunensis subsp. orientalis
DOI 10.1016/j.vetmic.2015.10.007
Authors Esteban Soto, Iona Halliday-Wimmonds, Stewart Francis, Michael T. Kearney, John D. Hansen
Publication Type Article
Publication Subtype Journal Article
Series Title Veterinary Microbiology
Index ID 70159728
Record Source USGS Publications Warehouse
USGS Organization Western Fisheries Research Center