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Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America

November 13, 2019

We developed two quantitative PCR assays for use with environmental DNA (eDNA) to detect numerous species in the genus Cottus that are indigenous to the Pacific coast watersheds of North America. We conducted in vitro assay validations on ten Cottus species and 32 potentially co-occurring non-Cottus species. We demonstrate the efficacy of these assays by field testing eDNA samples collected from streams inhabited by Cottus. These assays will be particularly useful for detecting Cottus in habitats where one (or more) of several Cottus species could be present.

Citation Information

Publication Year 2020
Title Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America
DOI 10.1007/s12686-019-01118-7
Authors Marshal Hoy, Carl Ostberg
Publication Type Article
Publication Subtype Journal Article
Series Title Conservation Genetics Resources
Series Number
Index ID 70206718
Record Source USGS Publications Warehouse
USGS Organization Western Fisheries Research Center

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