We developed two quantitative PCR assays for use with environmental DNA (eDNA) to detect numerous species in the genus Cottus that are indigenous to the Pacific coast watersheds of North America. We conducted in vitro assay validations on ten Cottus species and 32 potentially co-occurring non-Cottus species. We demonstrate the efficacy of these assays by field testing eDNA samples collected from streams inhabited by Cottus. These assays will be particularly useful for detecting Cottus in habitats where one (or more) of several Cottus species could be present.
Citation Information
| Publication Year | 2020 |
|---|---|
| Title | Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America |
| DOI | 10.1007/s12686-019-01118-7 |
| Authors | Marshal Hoy, Carl Ostberg |
| Publication Type | Article |
| Publication Subtype | Journal Article |
| Series Title | Conservation Genetics Resources |
| Index ID | 70206718 |
| Record Source | USGS Publications Warehouse |
| USGS Organization | Western Fisheries Research Center |
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Marshal Hoy
Biological Science Laboratory Technician
Biological Science Laboratory Technician
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Carl Ostberg, Ph.D.
Research Fish Biologist
Research Fish Biologist
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Marshal Hoy
Biological Science Laboratory TechnicianEmailPhoneCarl Ostberg, Ph.D.
Research Fish BiologistEmailPhone