Effective management of deep-sea ecosystems and the high seas is hindered by the absence of a globally consistent framework for characterizing benthic habitats. Here we present the first global ecological classification of the seafloor, comprising 250 unique ecological benthic units (EBUs), distributed on the seafloor as nearly 700,000 EBU polygon occurrences, generated by intersecting a high-resolution geomorphic map with multivariate environmental seascapes. Using 17 million seafloor data points and 0.05° resolution biophysical datasets—including bottom temperature, dissolved oxygen, pH, carbon flux, sediment thickness, crustal age, and bottom currents—we identified 57 benthic regions across six major geomorphic groups (shelves, slopes, seamounts/guyots, spreading ridges, abyssal/hadal areas, plateaus). The resulting EBUs reveal previously unrecognized ecological gradients, quantify global patterns of benthic heterogeneity, and expose large-scale environmental vulnerabilities. Notably, we find that 95.6 million km2 (26% of the ocean area) of abyssal seafloor lies below the carbonate compensation depth, that 4.16 million km2 (1% of the ocean) of continental slopes intersect severe oxygen minima, and <1% of seamounts occur in seascapes most environmentally favorable to life. These insights provide a powerful basis for identifying rare habitat configurations, assessing exposure to climate-driven stressors, and prioritizing areas for high seas marine protected area planning, as well as a policy-relevant foundation for environmental impact assessment and biodiversity baseline proxies under the new United Nations High Seas Treaty.
