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Effects of polychlorinated biphenyls, hexachlorocyclohexanes, and mercury on human neutrophil apoptosis, actin cytoskelton, and oxidative state

January 1, 2006

Apoptosis, or programmed cell death, has been proposed as a biomarker for environmental contaminant effects. In this work, we test the hypothesis that in vitro assays of apoptosis are sensitive indicators of immunological effects of polychlorinated biphenyls, hexachlorocyclohexanes, and mercury on human neutrophils. Apoptosis, necrosis, and viability as well as the related indicators F-actin levels, and active thiol state were measured in purified human neutrophils after treatment with contaminants. Effective concentrations observed were 0.3 μM (60 μg/L) mercury, 750 μg/L Aroclor 1254, and 50 μM (14,500 μg/L) hexachlorocylcohexanes. Concentrations of contaminants that induced apoptosis also decreased cellular F-actin levels. Active thiols were altered by mercury, but not organochlorines. Comparison of these data with levels of contaminants reported to be threats to human health indicate neutrophil apoptosis is a sensitive indicator of mercury toxicity.

Citation Information

Publication Year 2006
Title Effects of polychlorinated biphenyls, hexachlorocyclohexanes, and mercury on human neutrophil apoptosis, actin cytoskelton, and oxidative state
DOI 10.1016/j.etap.2006.03.005
Authors L.I. Sweet, D. R. Passino-Reader, P.G. Meier, G.M. Omann
Publication Type Article
Publication Subtype Journal Article
Series Title Environmental Toxicology and Pharmacology
Series Number
Index ID 70028483
Record Source USGS Publications Warehouse
USGS Organization Great Lakes Science Center