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An inducible HSP70 gene from the midge Chironomus dilutus: Characterization and transcription profile under environmental stress

January 1, 2009

In the present study, we identified and characterized an inducible heat shock protein 70 (HSP70) from the midge Chironomus dilutus and investigated the transcriptional profile of the gene under baseline and environmentally stressful conditions. Using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), we observed increased expression of CD-HSP70-1 in response to both heat shock and copper stress. We also investigated the expression of this gene during midge development. All C. dilutus developmental stages expressed CD-HSP70-1 under normal conditions, although at extremely low levels. Phylogenetic analysis of the amino acid sequence demonstrated distinct clustering of this gene with inducible HSP70s from other insect species. ?? 2008 The Authors.

Citation Information

Publication Year 2009
Title An inducible HSP70 gene from the midge Chironomus dilutus: Characterization and transcription profile under environmental stress
DOI 10.1111/j.1365-2583.2008.00853.x
Authors N. K. Karouna-Renier, K.R. Rao
Publication Type Article
Publication Subtype Journal Article
Series Title Insect Molecular Biology
Series Number
Index ID 70032271
Record Source USGS Publications Warehouse
USGS Organization