Little is known about nitric oxide (NO) production or consumption in the subsurface, an environment which may be conducive to NO accumulation. A study conducted in a nitrogen-contaminated aquifer on Cape Cod, Massachusetts assessed the occurrence and turnover of NO within a contaminant plume in which nitrification and denitrification were known to occur. NO (up to 8.6 nM) was detected in restricted vertical zones located within a nitrate (NO3−) gradient and characterized by low dissolved oxygen (