Development of methods distinguishing live and dead cell DNA for qPCR detection and quantification of pathogen genes in environmental samples for quantitative microbial risk assessment- Carrie Givens

Molecular methods such as quantitative polymerase chain reaction (qPCR) allow scientists the ability to target specific genes allowing for a rapid, sensitive, and cost-effective way to determine the presence and relative abundance of potential pathogen genes in the environment. Although there are many benefits to qPCR such as not relying on the ability to culture fastidious microorganisms ...