Donald Tillitt, PhD (Former Employee)
Science and Products
Filter Total Items: 215
Reproductive condition and occurrence of intersex in bighead carp and silver carp in the Missouri River Reproductive condition and occurrence of intersex in bighead carp and silver carp in the Missouri River
Little is known about the reproductive biology of the exotic bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix in the Missouri River. In order to fill this gap in understanding, herein is described the reproductive condition of these Asian carps. Evidence is presented which indicates that bighead and silver carp in the Missouri River have a protracted...
Authors
D. M. Papoulias, D. Chapman, D. E. Tillitt
Temporal and spatial variation of early mortality syndrome in salmonids from Lakes Michigan and Huron Temporal and spatial variation of early mortality syndrome in salmonids from Lakes Michigan and Huron
To assess the extent that early mortality syndrome (EMS) impacts different Pacific salmonid stocks and the association of EMS with thiamine, we collected eggs of coho salmon Oncorhynchus kisutch from three Lake Michigan tributaries (Platte River, Thompson Creek, and Root River) in 1996-2001. We also obtained eggs of Chinook salmon O. tshawytscha from Lake Michigan (Little Manistee River)...
Authors
M. Wolgamood, J.G. Hnath, S.B. Brown, K. Moore, S.V. Marcquenski, D. C. Honeyfield, J. P. Hinterkopf, J.D. Fitzsimons, D. E. Tillitt
Effect of sample handling on thiamine and thiaminolytic activity in alewife Effect of sample handling on thiamine and thiaminolytic activity in alewife
Alewives Alosa pseudoharengus were collected to evaluate handling and processing conditions that may affect the measurement of their thiamine-thiaminase content. Fish were captured by otter trawl, and reference samples of live fish were quick-frozen on dry ice immediately following capture. Other samples were placed on wet ice (4??C) or held in ambient lake water (21.5??C) for periods of...
Authors
G.M. Wright, S.B. Brown, L. R. Brown, K. Moore, M. Villella, J.L. Zajicek, D. E. Tillitt, J.D. Fitzsimons, D. C. Honeyfield
A rapid solid-phase extraction fluorometric method for thiamine and riboflavin in salmonid eggs A rapid solid-phase extraction fluorometric method for thiamine and riboflavin in salmonid eggs
A new method has been developed and successfully applied to the selective measurement of thiamine (nonphosphorylated), total thiamine (sum of thiamine, thiamine monophosphate [TMP], thiamine diphosphate [TDP], and thiamine triphosphate [TTP]), and potentially interfering riboflavin in acidic (2% trichloroacetic acid) extracts of selected salmonid and walleye egg samples. Acidic extracts...
Authors
James L. Zajicek, Donald E. Tillitt, Scott B. Brown, Lisa R. Brown, Dale C. Honeyfield, John D. Fitzsimons
A method for measuring total thiaminase activity in fish tissues A method for measuring total thiaminase activity in fish tissues
An accurate, quantitative, and rapid method for the measurement of thiaminase activity in fish samples is required to provide sufficient information to characterize the role of dietary thiaminase in the onset of thiamine deficiency in Great Lakes salmonines. A radiometric method that uses 14C-thiamine was optimized for substrate and co-substrate (nicotinic acid) concentrations...
Authors
James L. Zajicek, Donald E. Tillitt, Dale C. Honeyfield, Scott B. Brown, John D. Fitzsimons
Early mortality syndrome in Great Lakes salmonines Early mortality syndrome in Great Lakes salmonines
Early mortality syndrome (EMS) is the termused to describe an embryonic mortality affectingthe offspring of salmonines (coho salmonOnco-rhynchus kisutch, Chinook salmonOncorhynchustshawytscha, steelhead [anadromous rainbow troutOncorhynchus mykiss], brown troutSalmo trutta,and lake trout,Salvelinus namaycush) in LakesMichigan and Ontario and, to a lesser extent, LakesHuron and Erie...
Authors
Dale C. Honeyfield, Scott B. Brown, John D. Fitzsimons, Donald E. Tillitt
Science and Products
Filter Total Items: 215
Reproductive condition and occurrence of intersex in bighead carp and silver carp in the Missouri River Reproductive condition and occurrence of intersex in bighead carp and silver carp in the Missouri River
Little is known about the reproductive biology of the exotic bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix in the Missouri River. In order to fill this gap in understanding, herein is described the reproductive condition of these Asian carps. Evidence is presented which indicates that bighead and silver carp in the Missouri River have a protracted...
Authors
D. M. Papoulias, D. Chapman, D. E. Tillitt
Temporal and spatial variation of early mortality syndrome in salmonids from Lakes Michigan and Huron Temporal and spatial variation of early mortality syndrome in salmonids from Lakes Michigan and Huron
To assess the extent that early mortality syndrome (EMS) impacts different Pacific salmonid stocks and the association of EMS with thiamine, we collected eggs of coho salmon Oncorhynchus kisutch from three Lake Michigan tributaries (Platte River, Thompson Creek, and Root River) in 1996-2001. We also obtained eggs of Chinook salmon O. tshawytscha from Lake Michigan (Little Manistee River)...
Authors
M. Wolgamood, J.G. Hnath, S.B. Brown, K. Moore, S.V. Marcquenski, D. C. Honeyfield, J. P. Hinterkopf, J.D. Fitzsimons, D. E. Tillitt
Effect of sample handling on thiamine and thiaminolytic activity in alewife Effect of sample handling on thiamine and thiaminolytic activity in alewife
Alewives Alosa pseudoharengus were collected to evaluate handling and processing conditions that may affect the measurement of their thiamine-thiaminase content. Fish were captured by otter trawl, and reference samples of live fish were quick-frozen on dry ice immediately following capture. Other samples were placed on wet ice (4??C) or held in ambient lake water (21.5??C) for periods of...
Authors
G.M. Wright, S.B. Brown, L. R. Brown, K. Moore, M. Villella, J.L. Zajicek, D. E. Tillitt, J.D. Fitzsimons, D. C. Honeyfield
A rapid solid-phase extraction fluorometric method for thiamine and riboflavin in salmonid eggs A rapid solid-phase extraction fluorometric method for thiamine and riboflavin in salmonid eggs
A new method has been developed and successfully applied to the selective measurement of thiamine (nonphosphorylated), total thiamine (sum of thiamine, thiamine monophosphate [TMP], thiamine diphosphate [TDP], and thiamine triphosphate [TTP]), and potentially interfering riboflavin in acidic (2% trichloroacetic acid) extracts of selected salmonid and walleye egg samples. Acidic extracts...
Authors
James L. Zajicek, Donald E. Tillitt, Scott B. Brown, Lisa R. Brown, Dale C. Honeyfield, John D. Fitzsimons
A method for measuring total thiaminase activity in fish tissues A method for measuring total thiaminase activity in fish tissues
An accurate, quantitative, and rapid method for the measurement of thiaminase activity in fish samples is required to provide sufficient information to characterize the role of dietary thiaminase in the onset of thiamine deficiency in Great Lakes salmonines. A radiometric method that uses 14C-thiamine was optimized for substrate and co-substrate (nicotinic acid) concentrations...
Authors
James L. Zajicek, Donald E. Tillitt, Dale C. Honeyfield, Scott B. Brown, John D. Fitzsimons
Early mortality syndrome in Great Lakes salmonines Early mortality syndrome in Great Lakes salmonines
Early mortality syndrome (EMS) is the termused to describe an embryonic mortality affectingthe offspring of salmonines (coho salmonOnco-rhynchus kisutch, Chinook salmonOncorhynchustshawytscha, steelhead [anadromous rainbow troutOncorhynchus mykiss], brown troutSalmo trutta,and lake trout,Salvelinus namaycush) in LakesMichigan and Ontario and, to a lesser extent, LakesHuron and Erie...
Authors
Dale C. Honeyfield, Scott B. Brown, John D. Fitzsimons, Donald E. Tillitt
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