Two tables of data are included in this data release. The first table provides screening results for 32 previously described and/or published microsatellites and 1 sexing marker, primer redesigns for use in Mexican Spotted Owls in this data release, and size of markers in Mexican Spotted Owls. The second table provides microsatellite and sexing marker results for 10 tissue and 98 feather samples for 11 microsatellite markers identified as variable in initial marker screenings.