Fish fin and muscle tissue natural abundance stable isotope data for four prairie fishes native to the upper Red River basin of Texas and Oklahoma, USA, including plains minnow (Hybognathus placitus), prairie chub (Macrhybopsis australis), Red River shiner (Notropis bairdi), and Red River pupfish (Cyprinodon rubrofluviatilis). Fish fin and muscle tissue were analyzed for 13C and 15N isotopes. Also included are length, mass, and age estimates for each specimen. Ages were estimated by examining growth increments on otoliths.