This data release includes metadata and tabular data that documents the response of ohia (Metrosideros polymorpha) and ambrosia beetles (Curculionidae, Xyleborini) following the intentional inoculation of healthy ohia to Ceratocystis lukuohia, one of two fungal pathogens that causes Rapid Ohia Death (ROD). Ohia were inoculated with the ROD pathogen at three sites differing in elevation on the Island of Hawaii during January and June 2019. Inoculated and control trees were monitored for changes in foliage condition, beetle attack rates, beetle species composition, and the viability of the fungi within the tree through August 2021. These data are represented in the following seven files: 1) descriptions of trees, 2) tree conditions and the presence of beetle galleries, 3) beetles collected in boring dust traps (BDTs), 4) viability of fungi in beetle frass, 5) beetles collected on sticky traps, 6) descriptions of felled trees, and 7) beetles emerging from felled trees.