These data were compiled to better understand the controls on the magnitude of the metalimnion low dissolved oxygen zone in Lake Powell reservoir. These data represent dissolved oxygen, total suspended sediment, and nutrient data collected during laboratory water and sediment incubations. These data also represent summaries of summertime metalimnion dissolved oxygen concentrations at seven sites in Lake Powell, largely drawing from a pre-existing data release. These data were collected in Lake Powell reservoir, in the inflow regions where the Colorado River and the San Juan River enter Lake Powell, and from the Chinle Wash, which flows into the San Juan River before its confluence with Lake Powell by the U.S. Geological Survey and in collaboration with the Bureau of Reclamation and the National Park Service. These data can be used to quantify dissolved oxygen demand and nutrient transformations in the Lake Powell inflows. These data can also be used to evaluate patterns in summertime metalimnion dissolved oxygen, although we recommend users consult the original data release (Andrews and Deemer 2024) cited herein for this purpose given the more extensive nature of that dataset.