These data were compiled or measured (depending on the trait) for 110 plant species which were documented in vegetation monitoring surveys in years 2012-2014 along the Colorado River through Grand Canyon National Park. New trait data was collected forspecific leaf area, stem specific gravity, δ13C, δ15N, percent carbon and nitrogen, and carbon/nitrogen ratio. Seed mass and plant height data were collected from the literature and the KEW Royal Botanic Garden Seed Information Database (http://data.kew.org/sid/). The purpose of these data are to examine how species-level functional traits vary across wetland indicator status categories, but can be used for a wide variety of trait-based analyses. These data are associated with the journal manuscript: McCoy-Sulentic M.E., Kolb T.E., Merritt D.M., Palmquist E.C., Ralston B.E., Sarr D.A., Variation in species-level plant functional traits over wetland indicator status categories: Ecology and Evolution, v. (online) doi: 10.1002/ece3.2975, https://doi.org/10.1002/ece3.2975.