Species differ in their habitat preferences based on seasonal hydrologic conditions. Species such as the semi-aquatic marsh rice rat (Oryzomys palustris) are adapted to high water conditions, while other species, such as the hispid cotton rat (Sigmodon hispidus) and the cotton mouse (Peromyscus gossypinus) prefer drier hammocks during the wet season. The aim of our study was to understand the status of rodent species in Everglades National Park. Specifically, we estimated the presence and density of three focal species: marsh rice rat, hispid cotton rat, and cotton mouse.