A study was conducted during 2004 to examine soil carbon storage of Prairie Pothole Region wetland catchments. These data represent the soil profile descriptions performed during that study; the remaining data were published previously (https://doi.org/10.5066/F7KS6QG2). Soil profile descriptions were performed at 270 temporary, seasonal, and semipermanent wetland catchments distributed throughout the Glaciated Plains and Missouri Coteau physiographic regions of the Prairie Pothole Region. Data were collected from four to six wetland and upland zones of the catchment. Study sites included cropland, restored grassland (formerly cropland), and native prairie (no cultivation history) catchments located in Iowa, Minnesota, Montana, North Dakota, and South Dakota.