These data are a part of the effort to map geologic substrates of the Stellwagen Bank National Marine Sanctuary region off Boston, Massachusetts. The overall goal is to develop high-resolution (1:25,000) interpretive maps, based on multibeam sonar data and seabed sampling, showing surficial geology and seabed sediment dynamics. The work was conducted in collaboration with the Stellwagen Bank National Marine Sanctuary, and the data collected will aid research on the ecology of fish and invertebrate species that inhabit the region. The Sanctuary's research vessel, R/V Auk, visited a total of 233 locations on Stellwagen Bank during six separate surveys from May 2016 to April 2019. During these surveys a customized Van Veen grab sampler (SEABOSS) equipped with a video camera and a CTD was deployed in drift mode to collect sediment for grain-size analysis, video imagery of the seabed, and measurements of water column properties including temperature, salinity, density, and fluorescence.