Data includes finescale (1.5 cm minimum resolution) vertical solute concentrations for filtered methylmercury (ng/L), filtered total mercury (ng/L), dissolved organic carbon (mg/L), and dissolved chloride (micromole/L) in SixMile Brook, New York and McTier Creek, South Carolina. Vertical solute data were assessed one time at one site in each stream in 2009, at edge-of-water (margin) and center-of-channel (channel) using two mini-piezometer (USGS MINIPOINT) devices (seven 0.325 cm stainless steel tubes, 0.8 cm screened interval). Each MINIPOINT device was installed with sampling points in surface water and at approximately 1.5, 4.5, 7.5, 12.5, 17.5, 30, and 60 cm below the bed-sediment/surface water interface.