Capabilities

By Organic Geochemistry Research Laboratory (OGRL)

Capabilities

Organic Geochemistry Research Laboratory (OGRL)

Kansas Water Science Center, Lawrence, KS

FY 2026 Services

Environmental Organic Chemistry (EOC) Unit

For additional information contact: Julie Dietze 785-832-3564, juliec@usgs.gov or

GS-W-KS_OGRL_EOC@usgs.gov

All internal Quality Control data must be requested during the establishment of the laboratory work agreement or additional fees may apply.

Requested spikes are assessed at the same price per sample as environmental samples.

Samples submitted and then later requested not to be analyzed must be submitted in email within 3 days of sample submission or the sample will be assessed analysis costs.

Re-analysis requests must be provided within 30 days of data delivery.

Original sample bottles will be disposed 12 months after delivery of data unless otherwise agreed upon in the established laboratory work agreement.

The OGRL must be notified by the end of Quarter 3 of the current fiscal year of any changes to the estimated sample totals in the established laboratory work agreement or additional fees may apply.

Routine Methods

Analysis Code: LCGY

Glyphosate, AMPA, and Glufosinate Method

Sample Prep: Derivatization (FMOC)

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water

Reporting Limit: 0.02 µg/L

OGRL Method Code: LCM57

Bottle Requirements: 1-20mL VOA amber borosilicate glass vial (filled ½ full) or 1-4oz (125mL) amber glass with Teflon lined caps (filled ½ full).

Compound Name/ CAS#: Parameter Code
Glyphosate/ 1071-83-6: 62722	*Aminomethylphosphonic Acid (AMPA)/ 1066-51-9: 62649	Glufosinate/ 51276-47-2: 62721

Analysis Code: LCAZ

Atrazine Method

Instrumentation: Direct inject liquid chromatography/tandem mass spectrometry

Matrix: Water

Reporting Limit: 0.05 µg/L

OGRL Method Code: LM127

Bottle Requirements: 1- 4oz (125mL) amber glass with Teflon lined caps (filled ½ full)

Compound/CAS#: Parameter Code
Atrazine/ 1912249: 39632

Analysis Code: LCTR

Direct Inject Water Method

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water

Reporting Limit: 2.0 ng/L

OGRL Method Code: LM129

Bottle Requirements: Contact Rachael Lane (785-760-4540; rflane@usgs.gov) for details about bottles or additional tire wear compounds of interest

Compound/CAS#: Parameter Code
6PPD-quinone / 2754428-18-5, 39944 6PPD/ 793-24-8, 39945

Other tire-related compounds available by request

Analysis Code: LCTR-EPA

EPA Draft Method 1634

Sample Prep: Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water (predominantly stormwater and surface water)

Reporting Limit: 0.200 ng/L

OGRL Method Code: LM128

Bottle Requirements: Contact Rachael Lane (785-760-4540; rflane@usgs.gov) for details about bottles or additional tire wear compounds of interest

Compound/CAS#: Parameter Code
6PPD-quinone / 2754428-18-5, 39943

Analysis Code: LCAB

Four Class Antibiotic Method

Sample Prep: Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water

Reporting Limit: 0.005 µg/L (except where noted)

Method Notes:

The concentrations for the family of isomers for chlortetracycline, oxytetracycline, and tetracycline are processed with the following differences and assumptions:

Only one isomer per family is physically added in the standard mix and standard addition solutions (isochlotetracycline, oxytetracycline, tetracycline)

Equal instrument response for each isomer in a family is assumed

Concentration is calculated on the sum of all isomers in a family and proportioned by area of individual isomer

Bottle Requirements: 1-4oz (125mL) amber glass with Teflon lined caps

Compound Name/ CAS#: Parameter Code, Method Code
Fluoroquinolines Ciprofloxacin/85721-33-1: 62898, LCM48 Enrofloxacin/ 93106-60-6: 66495 LCM48 Lomefloxacin/ 98079-51-7: 62900, LCM48 Norfloxacin/ 70458-96-7: 62757, LCM48 Ofloxacin/ 82419-36-1: 62899, LCM48 Sarafloxacin/ 98105-99-8: 62771, LCM48	Macrolides Azithromycin/ 83905-01-5: 62792, LCM94 Erythromycin (0.01 µg/L)/ 114-07-8: 62797, LCM95 *Erythromycin-H₂O (0.02 µg/L)/ 23893-13-2: 63674, LCM95 Roxithromycin/ 80214-83-1: 62895, LCM48 Tylosin (0.01 µg/L)/ 1401-69-0: 62896, LCM95 Virginiamycin/ 11006-76-1: 62897, LCM48
Sulfonamides Sulfachloropyridazine/ 80-32-0: 62774, LCM48 Sulfadiazine (0.05 µg/L)/ 68-35-9: 62963, LCM95 Sulfadimethoxine/ 122-11-2: 62776, LCM48 Sulfamethoxazole/ 723-46-6: 62775, LCM48 Sulfamethazine/ 57-68-1: 61762, LCM48 Sulfathiazole (0.05 µg/L)/ 72-14-0: 62778, LCM95	Tetracyclines Chlortetracycline (0.01 µg/L)/ 57-62-5: 61744: LCM95 Epi-chlortetracycline (0.01 µg/L)/ 101342-45-4: 63731, LCM95 Epi-iso-chlortetracycline (0.01 µg/L)/ N/A: 64047, LCM95 Iso-chlortetracycline/ 514-53-4: 64175, LCM94 Oxytetracycline/ 79-57-2: 61759, LCM94 Epi-oxytetracycline (0.01 µg/L)/ 35259-39-3: 63729, LCM95 Tetracycline/ 60-54-8: 62781, LCM94 *Epi-tetracycline (0.01 µg/L)/ 79-85-6: 63727, LCM95 Doxycycline (0.01 µg/L)/ 564-25-0: 62694, LCM95
Pharmaceuticals Carbamazepine/ 298-46-4: 62793, LCM48 Ibuprofen (0.05 µg/L)/ 15687-27-1: 62014, LCM95	Others Chloramphenicol (0.01 µg/L)/ 56-75-7: 65194, LCM95 Lincomycin/ 154-21-2: 62894, LCM48 Ormetoprim/ 6981-18-6: 62962, LCM48 Trimethoprim/ 738-70-5: 62023, LCM48

*Degradation product

Analysis Code:  LCSU  

Artificial Sweeteners Method 

Sample Prep: Solid-phase extraction  

Instrumentation: Liquid chromatography/tandem mass spectrometry 

Matrix: Water 

OGRL Method Code: LM100 

Bottle Requirements: 4-4oz (125mL) amber glass with Teflon lined caps  

Compound/CAS#/Parameter Code
Acesulfame-K (0.01 µg/L reporting limit)/ 55589-62-3/54209  Aspartame (0.002 µg/L reporting limit)/ 22839-47-0/54210	Neotame (0.0003 µg/L reporting limit)/ 165450-17-9/54211  Saccharin (0.02 µg/L reporting limit)/ 81-07-2/54212
	Sucralose (0.1 µg/L reporting limit)/ 56038-13-2/54213

Analysis Code: LCHM_PG

Progestins Method

Sample Prep: Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water

Reporting Limit: 1 ng/L (except where noted)

OGRL Method Code: LCM96 (except where noted)

Bottle Requirements: 6-4oz (125mL) amber glass with Teflon lined caps or 1-1 Liter amber glass bottle with Teflon lined cap.

Compound Name/ CAS#: Parameter Code
Desogestrel (10 ng/L)/ 54024-55-5: 53535, Method Code: LCM97 Drospirenone (10 ng/L)/ 67392-87-4: 53536, Method Code: LCM97 Gestodene/ 60282-87-3: 53537 Levonorgestrel/ 797-63-7: 53538 Medroxyprogesterone/ 520-85-4: 53539	Melengestrol acetate/ 2919-66-6: 53540 Nestorone/ 7759-35-5: 53541 19-Norethindrone/ 68-22-4: 53543 Norgestimate/ 35189-28-7: 53544 Progesterone/ 57-83-0: 64523

Analysis Code: LCHM2

Estrogens, Androgens, & Conjugates Method

Sample Prep: Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water

Reporting Limit: 1 ng/L (except where noted)

OGRL Method Code: LCM96

Bottle Requirements: 6-4oz (125mL) amber glass with Teflon lined caps or 1-1 Liter amber glass bottle with Teflon lined cap.

Compound Name/ CAS#: Parameter Code
Estrogens Trans-diethylstilbestrol/ 56-53-1: 64516 17α-Estradiol (17αE2)/ 57-91-0: 64508 17β-Estradiol (17βE2)/ 50-28-2: 64510 Estriol (E3)/ 50-27-1: 64520 Estrone (E1)/ 53-16-7: 64521 17α-Ethynylestradiol (EE2)/ 57-63-6: 64509 Equilenin/ 517-09-9: 64518 Equilin/ 474-86-2: 64519	Androgens Androstenedione/ 63-05-8: 64513 5α-Dihydrotestosterone/ 521-18-6: 53517 Epitestosterone/481-30-1: 64517 11-Ketotestosterone/ 564-35-2: 64507 Testosterone/ 58-22-0: 64545 Trenbolone/ 10161-33-8: 64526 Trenbolone acetate / 10161-34-9: 53518
Conjugates Androsterone glucuronide/ 1852-43-3: 53519 Trans-diethylstilbestrol glucuronide/ 2408-40-4: 53520 17β-Estradiol-glucuronide/ 1806-98-0: 53521 Estriol-3-glucuronide/ 2479-91-6: 53522 Estrone glucuronide/ 2479-90-5: 53523 Ethynylestradiol-3-glucuronide/ 60134-76-1: 53524 Testosterone glucuronide/1180-25-2: 53515 Androsterone sulfate/ 2479-86-9: 53525 Equilenin sulfate/ 16680-48-1: 53526 Equilin sulfate/27540-07-4: 53527 17β-Estradiol-3-sulfate/ 481-96-9: 53528 17β-Estradiol-17-sulfate/ 3233-69-0: 53529 Estriol-3-sulfate/ 481-95-8: 53530 Estriol-17-sulfate/ 42028-21-7: 53531 Estrone-3-sulfate/ 481-97-0: 53532 Ethynylestradiol-3-sulfate/ 24560-70-1: 53533 Testosterone sulfate/ 651-45-6: 53534

Compound Name/ CAS#: Parameter Code

Estrogens

Trans-diethylstilbestrol/ 56-53-1: 64516

17α-Estradiol (17αE2)/ 57-91-0: 64508

17β-Estradiol (17βE2)/ 50-28-2: 64510

Estriol (E3)/ 50-27-1: 64520

Estrone (E1)/ 53-16-7: 64521

17α-Ethynylestradiol (EE2)/ 57-63-6: 64509

Equilenin/ 517-09-9: 64518

Equilin/ 474-86-2: 64519

Androgens

Androstenedione/ 63-05-8: 64513

5α-Dihydrotestosterone/ 521-18-6: 53517

Epitestosterone/481-30-1: 64517

11-Ketotestosterone/ 564-35-2: 64507

Testosterone/ 58-22-0: 64545

Trenbolone/ 10161-33-8: 64526

Trenbolone acetate / 10161-34-9: 53518

Conjugates

Androsterone glucuronide/ 1852-43-3: 53519

Trans-diethylstilbestrol glucuronide/ 2408-40-4: 53520

17β-Estradiol-glucuronide/ 1806-98-0: 53521

Estriol-3-glucuronide/ 2479-91-6: 53522

Estrone glucuronide/ 2479-90-5: 53523

Ethynylestradiol-3-glucuronide/ 60134-76-1: 53524

Testosterone glucuronide/1180-25-2: 53515

Androsterone sulfate/ 2479-86-9: 53525

Equilenin sulfate/ 16680-48-1: 53526

Equilin sulfate/27540-07-4: 53527

17β-Estradiol-3-sulfate/ 481-96-9: 53528

17β-Estradiol-17-sulfate/ 3233-69-0: 53529

Estriol-3-sulfate/ 481-95-8: 53530

Estriol-17-sulfate/ 42028-21-7: 53531

Estrone-3-sulfate/ 481-97-0: 53532

Ethynylestradiol-3-sulfate/ 24560-70-1: 53533

Testosterone sulfate/ 651-45-6: 53534

Analysis Code: LCHM_MTox

Sample Prep: Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water

Reporting Limit: 1 ng/L (except where noted)

OGRL Method Code: LCM96

Bottle Requirements: 6-4oz (125mL) amber glass with Teflon lined caps or 1-1 Liter amber glass bottle with Teflon lined cap.

Compound Name/ CAS#: Parameter Code
Beauvericin (2 ng/L)/ 26048-05-5: 53551 Deoxynivalenol (2 ng/L)/ 51481-10-8: 53552 Nivalenol (2 ng/L)/ 23282-20-4: 53553 Verrucarin A/ 3148-09-02: 53554	α-Zearalanol/ 26538-44-3: 53555 α-Zearalenol/ 36455-72-8: 53556 β-Zearalenol/ 71030-11-0: 53557 Zearalenone/ 17924-92-4: 53558

Analysis Code: LCHM_PE

Phytoestrogens Method

Sample Prep: Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water

Reporting Limit: 1 ng/L

OGRL Method Code: LCM96

Bottle Requirements: 6-4oz (125mL) amber glass with Teflon lined caps or 1-1 Liter amber glass bottle with Teflon lined cap.

Compound Name/ CAS#: Parameter Code
Biochanin A/ 491-80-5: 53545 Coumestrol/ 479-13-0: 53546 Daidzein/ 486-66-8: 53547	Equol/ 531-95-3: 53548 Formononetin/ 485-72-3: 53549 Genistein/ 446-72-0: 53550

EOC Custom Request Research Methods: For additional information contact Rachael Lane, rflane@usgs.gov, 785-760-4540.

Analysis Code: LCPH

Pharmaceutical Method (other compounds available by request)

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water

Reporting Limit: 1 ng/L (except where noted)

Compound Name/ CAS#, Reporting Limit:
1,7-dimethylxanthine (5 ng/L)/611-59-6 Abacavir/ 136470-78-5 Acetaminophen (5 ng/L)/103-90-2 Acyclovir/59277-89-3 Albuterol/18559-94-9 Amphetamine/300-62-9 Atenolol/29122-68-7 Atrazine/1912-24-9 Bupropion/34911-55-2 Butalbital (10 ng/L)/77-26-9 Caffeine (10 ng/L)/58-08-2 Carbamazepine/298-46-4 Carisoprodol (2 ng/L)/78-44-4 Cefalexin/15686-71-2 Cimetidine/51481-61-9 Citalopram (10 ng/L) /59729-33-8	Erythromycin (5 ng/L)/114-07-8 Erythromycin-H2O (5 ng/L)/ 23893-13-2 Famotidine/76824-35-6 Fenofibrate/49562-28-9 Fexofenadine/83799-24-0 Fluconazole/86386-73-4 Fluoxetine (5 ng/L)/ 54910-89-3 Fluticasone propionate (5 ng/L)/ 80474-14-2 Gabapentin (10 ng/L)/60142-96-3 Gemfibrozil (10 ng/L)/25812-30-0 Glyburide/10238-21-8 Hydrochlorothiazide (10 ng/L)/58-93-5 Hydrocortisone (5 ng/L)/50-23-7 Hydroxybuproprion/92264-81-8	Norsertraline (20 ng/L) /87857-41-8 Nortramadol/75377-45-6 Norverapamil (5 ng/L)/67018-85-3 Oxcarbazepine/28721-07-5 Oxycodone/76-42-6 Penciclovir/39809-25-1 Pentoxifylline/1677687 Piperonyl butoxide (2 ng/L)/51-03-6 Propranolol/525-66-6 Pseudoephedrine/Ephedrine/90-82-4 & 299-42-3 Ractopamine/97825-25-7 Ranitidine/66357-35-5 Sertraline/79617-96-2 Sitagliptin/486460-32-6 Sulfamethoxazole/723-46-6
Clonidine (2 ng/L)/4205-90-7 Codeine/76-57-3 Cotinine (2 ng/L)/486-56-6 Dehydronifedipine/67035-22-7 Desacetyldiliazem/42399-40-6 Desloratadine (20 ng/L)/100643-71-8 Desmethyldiltiazem/86408-45-9 Desvenlafaxine (2 ng/L)/93413-62-8 Dextromethorphan/125-71-3 Diazepam (2 ng/L)/439-14-5 Diclofenac/15307-86-5 Diltiazem/42399-41-7 Diphenhydramine/147-24-0	Lamivudine (20 ng/L)/134678-17-4 Lamotrigine (5 ng/L)/84057-84-1 Lidocaine/137-58-6 Loratadine (5 ng/L)/79794-75-5 Meprobamate/57-53-4 Metformin (2 ng/L)/657-24-9 Methadone (10 ng/L)/76-99-3 Methocarbamol/532-03-6 Methyl-1h-benzotriazole/29385-43-1 Metoprolol/51384-51-1 Morphine (2 ng/L)/57-27-2 Nadolol/42200-33-9 N-desmethylcitalopram (5 ng/L)/62498-67-3 Nicotine (20 ng/L)/54-11-5	Tamoxifen (10 ng/L)/10540-29-1 Temazepam/846-50-4 Theophylline (10 ng/L)/58-55-9 Thiabendazole/148-79-8 Tiotropium/186691-13-4 Topiramate (10 ng/L)/97240-79-4 Tramadol/27203-92-5 Triamterene/396-01-0 Trimethoprim/738-70-5 Venlafaxine/93413-69-5 Verapamil (2 ng/L)/52-53-9

Analysis Code: LCTS

Solid Method

Sample Prep: Energized Dispersive Guided Extraction and Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Sediments and Soils

Reporting Limit:

OGRL Method Code: Not Assigned Yet

Compound/CAS#:
6PPD-quinone / 2754428-18-5 6PPD/ 793-24-8

Other tire-related compounds available by request

Analysis Code: LCGS

Solid Glyphosate, AMPA, and Glufosinate Method

Sample Prep: Derivatization (FMOC)

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Solids

Reporting Limit: 1-10 µg/kg

OGRL Method Code: LCM69

Compound Name/ CAS#, Parameter Code (Dry Weight)
Glyphosate/ 1071-83-6: 52168	*Aminomethylphosphonic Acid (AMPA)/ 1066-51-9: 52169	Glufosinate/ 51276-47-2: 52170

*Degradation product

Analysis Code: LCAS

Solid Four Class Antibiotic Method

Sample Prep: Pressurized liquid extraction, Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Solids

Compound Name:
Fluoroquinolines Ciprofloxacin Enrofloxacin Lomefloxacin Norfloxacin Ofloxacin Sarafloxacin	Macrolides Azithromycin Erythromycin *Erythromycin-H₂O Roxithromycin Tylosin Virginiamycin	Sulfonamides Sulfachloropyridazine Sulfadiazine Sulfadimethoxine Sulfamethoxazol Sulfamethazine Sulfathiazole
Tetracyclines -	Pharmaceuticals	Others
Chlorotetracycline Epi-chlorotetracycline Epi-iso-chlorotetracycline Iso-chlorotetracycline Oxytetracycline Epi-oxytetracycline Tetracycline *Epi-tetracycline Doxycycline	Carbamazepine Ibuprofen	Chloramphenicol Lincomycin Ormetoprim Trimethoprim

Compound Name:

Fluoroquinolines

Ciprofloxacin

Enrofloxacin

Lomefloxacin

Norfloxacin

Ofloxacin

Sarafloxacin

Macrolides

Azithromycin

Erythromycin

*Erythromycin-H₂O

Roxithromycin

Tylosin

Virginiamycin

Sulfonamides

Sulfachloropyridazine

Sulfadiazine

Sulfadimethoxine

Sulfamethoxazol

Sulfamethazine

Sulfathiazole

Tetracyclines -

Pharmaceuticals

Others

Chlorotetracycline

*Epi-chlorotetracycline

*Epi-iso-chlorotetracycline

*Iso-chlorotetracycline

Oxytetracycline

*Epi-oxytetracycline

Tetracycline

*Epi-tetracycline

Doxycycline

Carbamazepine

Ibuprofen

Chloramphenicol

Lincomycin

Ormetoprim

Trimethoprim

Analysis Code: LCHS2

Solid Estrogens, Androgens, & Conjugates Method

Sample Prep: Pressurized liquid extraction, Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Solids

OGRL Method Code: Not assigned yet

Compound Name/ CAS#: Parameter Codes not assigned yet
Estrogens Trans-diethylstilbestrol/ 56-53-1 17α-Estradiol (17αE2)/ 57-91-0 17β-Estradiol (17βE2)/ 50-28-2 Estriol (E3)/ 50-27-1 Estrone (E1)/ 53-16-7 17α-Ethinylestradiol (EE2)/ 57-63-6 Equilenin/ 517-09-9 Equilin/ 474-86-2	Androgens Androstenedione/ 63-05-8 5α-Dihydrotestosterone/ 521-18-6 Epitestosterone/ 481-30 11-Ketotestosterone/ 564-35-2 Testosterone/ 58-22-0 Trenbolone/ 10161-33-8 Trenbolone acetate/ 10161-34-9
Conjugates Androsterone glucuronide/ 1852-43-3 Trans-diethylstilbestrol glucuronide/ 2408-40-4 17β-estradiol-glucuronide/ 1806-98-0 Estriol-3-glucuronide/ 2479-91-6 Estrone glucuronide/ 2479-90-5 Ethynylestradiol-3-glucuronide/ 60134-76-1 Testosterone glucuronide/ 118-25-2 Androsterone sulfate/ 2479-86-9 Equilenin sulfate/ 16680-48-1 Equilin sulfate/ 16680-47-0 17β-estradiol-3-sulfate/ 481-96-9 17β-estradiol-17-sulfate/ 3233-69-0 Estriol-3-sulfate/ 481-95-8 Estriol-17-sulfate/ 42028-21-7 Estrone-3-sulfate/ 481-97-0 Ethynylestradiol-3-sulfate/ 24560-70-1 Testosterone sulfate/ 651-45-6

Compound Name/ CAS#: Parameter Codes not assigned yet

Estrogens

Trans-diethylstilbestrol/ 56-53-1

17α-Estradiol (17αE2)/ 57-91-0

17β-Estradiol (17βE2)/ 50-28-2

Estriol (E3)/ 50-27-1

Estrone (E1)/ 53-16-7

17α-Ethinylestradiol (EE2)/ 57-63-6

Equilenin/ 517-09-9

Equilin/ 474-86-2

Androgens

Androstenedione/ 63-05-8

5α-Dihydrotestosterone/ 521-18-6

Epitestosterone/ 481-30

11-Ketotestosterone/ 564-35-2

Testosterone/ 58-22-0

Trenbolone/ 10161-33-8

Trenbolone acetate/ 10161-34-9

Conjugates

Androsterone glucuronide/ 1852-43-3

Trans-diethylstilbestrol glucuronide/ 2408-40-4

17β-estradiol-glucuronide/ 1806-98-0

Estriol-3-glucuronide/ 2479-91-6

Estrone glucuronide/ 2479-90-5

Ethynylestradiol-3-glucuronide/ 60134-76-1

Testosterone glucuronide/ 118-25-2

Androsterone sulfate/ 2479-86-9

Equilenin sulfate/ 16680-48-1

Equilin sulfate/ 16680-47-0

17β-estradiol-3-sulfate/ 481-96-9

17β-estradiol-17-sulfate/ 3233-69-0

Estriol-3-sulfate/ 481-95-8

Estriol-17-sulfate/ 42028-21-7

Estrone-3-sulfate/ 481-97-0

Ethynylestradiol-3-sulfate/ 24560-70-1

Testosterone sulfate/ 651-45-6

Analysis Code: LCHS_PE

Solid Phytoestrogens Method

Sample Prep: Pressurized liquid extraction, Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Solids

OGRL Method Code: Not Assigned Yet

Compound Name/ CAS#: Parameter Codes not assigned yet
Biochanin A/ 491-80-5 Coumestrol/ 479-13-0 Daidzein/ 486-66-8	Equol/ 531-95-3 Formononetin/ 485-72-3 Genistein/ 446-72-0

Analysis Code: LCHS_PG

Solid Progestins Method

Sample Prep: Pressurized liquid extraction, Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Solids

OGRL Method Code: Not Assigned Yet

Compound Name/ CAS#: Parameter Codes not assigned yet
Desogestrel/ 54024-55-5 Drospirenone/ 67392-87-4 Gestodene/ 60282-87-3 Levonorgestrel/ 797-63-7 Medroxyprogesterone/ 520-85-4	Melengestrol acetate/ 2919-66-6 Nestorone/ 7759-35-5 19-Norethindrone/ 62-88-4 Norgestimate/ 35189-28-7 Progesterone/ 57-83-0

Analysis Code: LCHS_MTox

Solid Mycotoxin Method

Sample Prep: Pressurized liquid extraction, Solid-phase extraction

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Solids

OGRL Method Code: Not Assigned Yet

Compound Name/ CAS#: Parameter Codes not assigned yet
Beauvericin/ 26048-05-5 Deoxynivalenol/ 51481-10-8 Nivalenol/ 23282-20-4 Verrucarin A/ 3148-09-02	α-Zearalanol/ 26538-44-3 α-Zearalenol/ 36455-72-8 β-Zearalenol/ 71030-11-0 Zearalenone/ 17924-92-4

Compound Name/ CAS#: Parameter Codes not assigned yet

Beauvericin/ 26048-05-5

Deoxynivalenol/ 51481-10-8

Nivalenol/ 23282-20-4

Verrucarin A/ 3148-09-02

α-Zearalanol/ 26538-44-3

α-Zearalenol/ 36455-72-8

β-Zearalenol/ 71030-11-0

Zearalenone/ 17924-92-4

Psychoactive Compounds, Preliminary Method Development

Sample Prep: To be determined.

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water

Price Per Sample: To be determined.

Potential Compounds:
Amphetamines Amphetamine Cathinone Cocaine Benzoylecgonine Cocaethylene *Ecgonine Methyl Ester MDA MDEA MDMA Methamphetamine Phentermine	Benzodiazepines Alprazolam Chlordiazepoxide Clonazepam Diazepam Estazolam Flunitrazepam Lorazepam Midazolam Nitrazepam Oxazepam Temazepam Zolpidem	Barbiturates Amobarbital Butalbital Pentobarbital Phenobarbital Secobarbital Hallucinogens Ketamine Norketamine LSD 2-Oxo-3-hydroxy-LSD Methoxetamine	Opioids Buprenorphine Norbuprenorphine Glucuronide Codeine Fentanyl Heroin Hydrocodone Hydromorphone Mephedrone Methadone EDDP *EMDP Morphine Naloxone Oxycodone Oxymorphone Tramadol

Potential Compounds:

Amphetamines

Amphetamine

Cathinone

Cocaine

*Benzoylecgonine

*Cocaethylene

*Ecgonine Methyl Ester

MDA

MDEA

MDMA

Methamphetamine

Phentermine

Benzodiazepines

Alprazolam

Chlordiazepoxide

Clonazepam

Diazepam

Estazolam

Flunitrazepam

Lorazepam

Midazolam

Nitrazepam

Oxazepam

Temazepam

Zolpidem

Barbiturates

Amobarbital

Butalbital

Pentobarbital

Phenobarbital

Secobarbital

Hallucinogens

Ketamine

*Norketamine

LSD

*2-Oxo-3-hydroxy-LSD

Methoxetamine

Opioids

Buprenorphine

*Norbuprenorphine Glucuronide

Codeine

Fentanyl

Heroin

Hydrocodone

Hydromorphone

Mephedrone

Methadone

*EDDP

*EMDP

Morphine

Naloxone

Oxycodone

Oxymorphone

Tramadol

Cannabinoids, Preliminary Method Development

Research Method for Cannabis Compounds

Sample Prep: To be determined.

Instrumentation: Liquid chromatography/tandem mass spectrometry

Matrix: Water

Price Per Sample: To be determined.

Potential Compounds:
Cannabidiol Cannabinol	Tetrahydrocannabinol (THC) 11-Hydroxy-THC	11-Nor-9-Carboxy-THC

Potential Compounds:

Cannabidiol

Cannabinol

Tetrahydrocannabinol (THC)

11-Hydroxy-THC

11-Nor-9-Carboxy-THC

Algal and Environmental Toxins (AET) Unit

For additional information contact Zach Laughrey 785-832-3585, zlaughrey@usgs.gov

*All internal Quality Control data must be requested during the establishment of the sample processing agreement or additional fees may apply.

*Call to discuss analysis of Protein Phosphatase 2a Inhibition (785) 764-1408

ELISA techniques

OGRL Method Name: Description, Parameter Code, Method Code
Microcystin: CAS# 101043-37-2 IMNT2: Total Analysis, 89011, IMM26 IMNT2S: Total High Salinity, 89011, IMM28 IMND2: Dissolved Analysis, 89012, IMM27 IMND2S: Dissolved High Salinity, 89012, IMM29 Cylindrospermopsin: CAS# 143545-90-8 IMYT: Total Analysis, 89005, IMM10 IMYTS: Total High Salinity, 89005, IMM20 IMYD: Dissolved Analysis, 89006, IMM11 IMYDS: Dissolved High Salinity, 89006, IMM21 Saxitoxin/ 35523-89-8 IMXT: Total Analysis, 89003, IMM08 IMXTS: Total High Salinity, 89003, IMM22 IMXD: Dissolved Analysis, 89004, IMM09 IMXDS: Dissolved High Salinity, 89004, IMM23 Anatoxin-a/ 64285-06-9 IMAAT: Total Analysis, 53954, IMM24 IMAAD: Dissolved Analysis, 53955, IMM25

OGRL Method Name: Description, Parameter Code, Method Code

Microcystin: CAS# 101043-37-2

IMNT2: Total Analysis, 89011, IMM26

IMNT2S: Total High Salinity, 89011, IMM28

IMND2: Dissolved Analysis, 89012, IMM27

IMND2S: Dissolved High Salinity, 89012, IMM29

Cylindrospermopsin: CAS# 143545-90-8

IMYT: Total Analysis, 89005, IMM10

IMYTS: Total High Salinity, 89005, IMM20

IMYD: Dissolved Analysis, 89006, IMM11

IMYDS: Dissolved High Salinity, 89006, IMM21

Saxitoxin/ 35523-89-8

IMXT: Total Analysis, 89003, IMM08

IMXTS: Total High Salinity, 89003, IMM22

IMXD: Dissolved Analysis, 89004, IMM09

IMXDS: Dissolved High Salinity, 89004, IMM23

Anatoxin-a/ 64285-06-9

IMAAT: Total Analysis, 53954, IMM24

IMAAD: Dissolved Analysis, 53955, IMM25

Algal Toxins by Liquid chromatography/tandem mass spectrometry

LCTXO-MT-QT (Marine Toxins only: Total Analysis)

Sample Prep: Freeze/thaw x 3 then filter

Instrumentation: Liquid chromatography/High Resolution Mass Spectrometry via Orbitrap

Matrix: Water

Reporting Limit: 0.10µg/L (except where noted)

OGRL Method Code: LCM87

Compound Name/ CAS#: Parameter Code
13-Desmethyl spirolide C (0.08 µg/L)/ 334974-07-1: 53973 Azaspiracid-1/ 214899-21-5: 53969 Dinophysistoxin-1 (0.30 µg/L)/ 81720-10-7: 53970 Dinophysistoxin-2/ N/A: 53974 Domoic Acid (0.08 µg/L)/ 14277-97-5: 52687	Gymnodimine/ 173792-58-0: 53971 Nodularin R (NDLR)/ 118399-22-7: 52696 Okadaic Acid/ 78111-17-8: 52697 Pectenotoxin-2/ 97564-91-5: 53972

Compound Name/ CAS#: Parameter Code

13-Desmethyl spirolide C (0.08 µg/L)/ 334974-07-1: 53973

Azaspiracid-1/ 214899-21-5: 53969

Dinophysistoxin-1 (0.30 µg/L)/ 81720-10-7: 53970

Dinophysistoxin-2/ N/A: 53974

Domoic Acid (0.08 µg/L)/ 14277-97-5: 52687

Gymnodimine/ 173792-58-0: 53971

Nodularin R (NDLR)/ 118399-22-7: 52696

Okadaic Acid/ 78111-17-8: 52697

Pectenotoxin-2/ 97564-91-5: 53972

LCTXO-MD-QT (Marine Toxins only: Dissolved Analysis)

Sample Prep: Filtered

Instrumentation: Liquid chromatography/High Resolution Mass Spectrometry via Orbitrap

Matrix: Water

Reporting Limit: 0.10µg/L (except where noted)

OGRL Method Code: LCM87

Compound Name/ CAS#: Parameter Code
13-Desmethyl spirolide C (0.08 µg/L)/ 334974-07-1: 53979 Azaspiracid-1/ 214899-21-5: 53975 Dinophysistoxin-1 (0.30 µg/L)/ 81720-10-7: 53976 Dinophysistoxin-2/ N/A: 53980 Domoic Acid (0.08 µg/L)/ 14277-97-5: 65193	Gymnodimine 173792-58-0: 53977 Nodularin R (NDLR)/ 118399-22-7: 56189 Okadaic Acid/ 78111-17-8: 52164 Pectenotoxin-2/ 97564-91-5: 53978

Compound Name/ CAS#: Parameter Code

13-Desmethyl spirolide C (0.08 µg/L)/ 334974-07-1: 53979

Azaspiracid-1/ 214899-21-5: 53975

Dinophysistoxin-1 (0.30 µg/L)/ 81720-10-7: 53976

Dinophysistoxin-2/ N/A: 53980

Domoic Acid (0.08 µg/L)/ 14277-97-5: 65193

Gymnodimine 173792-58-0: 53977

Nodularin R (NDLR)/ 118399-22-7: 56189

Okadaic Acid/ 78111-17-8: 52164

Pectenotoxin-2/ 97564-91-5: 53978

LCTXO-CT-QT (Freshwater Toxins only: Total Analysis)

Sample Prep: Freeze/thaw x 3 then filter

Instrumentation: Liquid chromatography/High Resolution Mass Spectrometry via Orbitrap

Matrix: Water

Reporting Limit: 0.10µg/L (except where noted)

OGRL Method Code: LCM87

Compound Name/ CAS#: Parameter Code
Anatoxin-a (ANTA) (0.08 µg/L)/ 64285-06-9: 68287 Cylindrospermopsin (CYLS)/ 143545-90-8: 52686 Microcystin HtYR (MCHtYR)/ N/A: 52690 Microcystin HiLR (MCHiLR)/ N/A: 52836 Microcystin LA (MCLA)/ 96180-79-9: 52688 Microcystin LF (MCLF)/ 154037-70-4: 52694 Microcystin LR (MCLR)/ 101043-37-2: 63686	Microcystin LW (MCLW)/ 111755-37-4: 52685 Microcystin LY (MCLY)/ 123304-10-9: 52689 Microcystin RR (MCRR) (0.08 µg/L)/ 111755-37-4: 52695 Microcystin YR (MCYR)/ 101043-37-2: 52693 Nodularin R (NDLR)/ 118399-22-7: 52696

Compound Name/ CAS#: Parameter Code

Anatoxin-a (ANTA) (0.08 µg/L)/ 64285-06-9: 68287

Cylindrospermopsin (CYLS)/ 143545-90-8: 52686

Microcystin HtYR (MCHtYR)/ N/A: 52690

Microcystin HiLR (MCHiLR)/ N/A: 52836

Microcystin LA (MCLA)/ 96180-79-9: 52688

Microcystin LF (MCLF)/ 154037-70-4: 52694

Microcystin LR (MCLR)/ 101043-37-2: 63686

Microcystin LW (MCLW)/ 111755-37-4: 52685

Microcystin LY (MCLY)/ 123304-10-9: 52689

Microcystin RR (MCRR) (0.08 µg/L)/ 111755-37-4: 52695

Microcystin YR (MCYR)/ 101043-37-2: 52693

Nodularin R (NDLR)/ 118399-22-7: 52696

LCTXO-CD-QT (Freshwater Toxins only: Dissolved Analysis)

Sample Prep: Filtered

Instrumentation: Liquid chromatography/High Resolution Mass Spectrometry via Orbitrap

Matrix: Water

Reporting Limit: 0.10µg/L (except where noted)

OGRL Method Code: LCM87

Compound Name/ CAS#: Parameter Code
Anatoxin-a (ANTA) (0.08 µg/L)/ 64285-06-9: 65191 Cylindrospermopsin (CYLS)/ 143545-90-8: 65190 Microcystin HtYR (MCHtYR)/ N/A: 52161 Microcystin HiLR (MCHiLR)/ N/A: 52165 Microcystin LA (MCLA)/ 96180-79-9: 65188 Microcystin LF (MCLF)/ 154037-70-4: 65186 Microcystin LR (MCLR)/ 101043-37-2: 63685	Microcystin LW (MCLW)/ 111755-37-4: 65185 Microcystin LY (MCLY)/ 123304-10-9: 52163 Microcystin RR (MCRR) (0.08 µg/L)/ 111755-37-4: 64048 Microcystin YR (MCYR)/ 101043-37-2: 65187 Nodularin R (NDLR)/ 118399-22-7: 65189

Compound Name/ CAS#: Parameter Code

Anatoxin-a (ANTA) (0.08 µg/L)/ 64285-06-9: 65191

Cylindrospermopsin (CYLS)/ 143545-90-8: 65190

Microcystin HtYR (MCHtYR)/ N/A: 52161

Microcystin HiLR (MCHiLR)/ N/A: 52165

Microcystin LA (MCLA)/ 96180-79-9: 65188

Microcystin LF (MCLF)/ 154037-70-4: 65186

Microcystin LR (MCLR)/ 101043-37-2: 63685

Microcystin LW (MCLW)/ 111755-37-4: 65185

Microcystin LY (MCLY)/ 123304-10-9: 52163

Microcystin RR (MCRR) (0.08 µg/L)/ 111755-37-4: 64048

Microcystin YR (MCYR)/ 101043-37-2: 65187

Nodularin R (NDLR)/ 118399-22-7: 65189

LCTXO-AT-QT (Freshwater and Marine Toxins: Total Analysis)

Sample Prep: Freeze/thaw x 3 then filter

Instrumentation: Liquid chromatography/High Resolution Mass Spectrometry via Orbitrap

Matrix: Water

Reporting Limit: 0.10µg/L (except where noted)

OGRL Method Code: LCM87

Compound Name/ CAS#: Parameter Code
13-Desmethyl spirolide C/ 334974-07-1: 53973 Anatoxin-a (ANTA) (0.08 µg/L)/ 64285-06-9: 68287 Azaspiracid-1/ 214899-21-5: 53969 Cylindrospermopsin (CYLS)/ 143545-90-8: 52686 Dinophysistoxin-1/ 81720-10-7: 53970 Dinophysistoxin-2/ N/A: 53974 Domoic Acid/ 14277-97-5: 52687 Gymnodimine/ 173792-58-0: 53971 Microcystin HtYR (MCHtYR)/ N/A: 52690 Microcystin HiLR (MCHiLR)/ N/A: 52836 Microcystin LA (MCLA)/ 96180-79-9: 52688 Microcystin LF (MCLF)/ 154037-70-4: 52694	Microcystin LR (MCLR)/ 101043-37-2: 63686 Microcystin LW (MCLW)/ 111755-37-4: 52685 Microcystin LY (MCLY)/ 123304-10-9: 52689 Microcystin RR (MCRR) (0.08 µg/L)/ 111755-37-4: 52695 Microcystin YR (MCYR)/ 101043-37-2: 52693 Nodularin R (NDLR)/ 118399-22-7: 52696 Okadaic Acid/ 78111-17-8: 52697 Pectenotoxin-2/ 97564-91-5: 53972

Compound Name/ CAS#: Parameter Code

13-Desmethyl spirolide C/ 334974-07-1: 53973

Anatoxin-a (ANTA) (0.08 µg/L)/ 64285-06-9: 68287

Azaspiracid-1/ 214899-21-5: 53969

Cylindrospermopsin (CYLS)/ 143545-90-8: 52686

Dinophysistoxin-1/ 81720-10-7: 53970

Dinophysistoxin-2/ N/A: 53974

Domoic Acid/ 14277-97-5: 52687

Gymnodimine/ 173792-58-0: 53971

Microcystin HtYR (MCHtYR)/ N/A: 52690

Microcystin HiLR (MCHiLR)/ N/A: 52836

Microcystin LA (MCLA)/ 96180-79-9: 52688

Microcystin LF (MCLF)/ 154037-70-4: 52694

Microcystin LR (MCLR)/ 101043-37-2: 63686

Microcystin LW (MCLW)/ 111755-37-4: 52685

Microcystin LY (MCLY)/ 123304-10-9: 52689

Microcystin RR (MCRR) (0.08 µg/L)/ 111755-37-4: 52695

Microcystin YR (MCYR)/ 101043-37-2: 52693

Nodularin R (NDLR)/ 118399-22-7: 52696

Okadaic Acid/ 78111-17-8: 52697

Pectenotoxin-2/ 97564-91-5: 53972

LCTXO-AD-QT (Freshwater and Marine Toxins: Dissolved Analysis)

Sample Prep: Filtered

Instrumentation: Liquid chromatography/High Resolution Mass Spectrometry via Orbitrap

Matrix: Water

Reporting Limit: 0.10µg/L (except where noted)

OGRL Method Code: LCM87

Compound Name/ CAS#: Parameter Code
13-Desmethyl spirolide C/ 334974-07-1: 53979 Anatoxin-a (ANTA) (0.08 µg/L)/ 64285-06-9: 65191 Azaspiracid-1/ 214899-21-5: 53975 Cylindrospermopsin (CYLS)/ 143545-90-8: 65190 Dinophysistoxin-1/ 81720-10-7: 53976 Dinophysistoxin-2/ N/A: 53980 Domoic Acid/ 14277-97-5: 65193 Gymnodimine/ 173792-58-0: 53977 Microcystin HtYR (MCHtYR)/ N/A: 52161 Microcystin HiLR (MCHiLR)/ N/A: 52165 Microcystin LA (MCLA)/ 96180-79-9: 65188 Microcystin LF (MCLF)/ 154037-70-4: 65186	Microcystin LR (MCLR)/ 101043-37-2: 63685 Microcystin LW (MCLW)/ 111755-37-4: 65185 Microcystin LY (MCLY)/ 123304-10-9: 52163 Microcystin RR (MCRR) (0.08 µg/L)/ 111755-37-4: 64048 Microcystin YR (MCYR)/ 101043-37-2: 65187 Nodularin R (NDLR)/ 118399-22-7: 65189 Okadaic Acid/ 78111-17-8: 52164 Pectenotoxin-2/ 97564-91-5: 53978

Compound Name/ CAS#: Parameter Code

13-Desmethyl spirolide C/ 334974-07-1: 53979

Anatoxin-a (ANTA) (0.08 µg/L)/ 64285-06-9: 65191

Azaspiracid-1/ 214899-21-5: 53975

Cylindrospermopsin (CYLS)/ 143545-90-8: 65190

Dinophysistoxin-1/ 81720-10-7: 53976

Dinophysistoxin-2/ N/A: 53980

Domoic Acid/ 14277-97-5: 65193

Gymnodimine/ 173792-58-0: 53977

Microcystin HtYR (MCHtYR)/ N/A: 52161

Microcystin HiLR (MCHiLR)/ N/A: 52165

Microcystin LA (MCLA)/ 96180-79-9: 65188

Microcystin LF (MCLF)/ 154037-70-4: 65186

Microcystin LR (MCLR)/ 101043-37-2: 63685

Microcystin LW (MCLW)/ 111755-37-4: 65185

Microcystin LY (MCLY)/ 123304-10-9: 52163

Microcystin RR (MCRR) (0.08 µg/L)/ 111755-37-4: 64048

Microcystin YR (MCYR)/ 101043-37-2: 65187

Nodularin R (NDLR)/ 118399-22-7: 65189

Okadaic Acid/ 78111-17-8: 52164

Pectenotoxin-2/ 97564-91-5: 53978