Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): Characterization of a sex-linked (Z-specific) and 14 autosomal loci
Canada goose (Branta Canadensis) and harlequin duck (Histrionicus histrionicus) DNAs were digested with Sau3AI, and size selected (300-700 bp) fragments were ligated into BamHI-digested pBluscriptII KS+. The enrichment protocol of Ostrander et al.1 was followed. The resulting libraries were screened using a [ƴ-32P]ATP end-labelled (CA)20 oligonucleotides as a hybridization probe. Positive clones were sequenced using cycle-sequencing protocols (Epicentre Technologies, Madison, WI) and primers flanking the inserts. PCR primers were designed to amplify the repeat and yield amplification products of ≈100-200 bp. DNA samples were screened for variation at these loci using [ƴ-32P]ATP end-labelled primers. The products were resolved using 6% denaturing polyacrylamide gels and autoradiography.
Citation Information
| Publication Year | 1998 |
|---|---|
| Title | Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): Characterization of a sex-linked (Z-specific) and 14 autosomal loci |
| DOI | 10.1046/j.1365-2052.1998.00247.x |
| Authors | W.G. Buchholz, John M. Pearce, Barbara J. Pierson, Kim T. Scribner |
| Publication Type | Article |
| Publication Subtype | Journal Article |
| Series Title | Animal Genetics |
| Index ID | 1012977 |
| Record Source | USGS Publications Warehouse |
| USGS Organization | Alaska Biological Science Center; Alaska Science Center |