Effect of dietary biotin and lipid on growth, stamina, lipid metabolism and biotin-containing enzymes in brook trout (Salvelinus fontinalis)

Simultaneous dietary fat and biotin deficiencies in brook trout suppressed weight gain, feed/gain efficiency, swimming stamina, and total liver lipids. Dietary fat alone reduced body water, increased body fat and ash, reversed the elevating effect of biotin plus fat on liver palmitic and stearic acids, and widened the 16:0 to 16:1, 18:0 to 18:1, 18:2 to 20:4 and 18:3 to 22:5 liver fatty acid ratios. Fat also accentuated the accumulation of liver oleic and linoleic acids caused by biotin deprivation and apparently prevented docosapentaenoic acid biosynthesis in biotin-deprived trout. Fish fed fat with no biotin had the least, and those fed biotin with no fat the most, liver acetyl CoA carboxylase (ACC) and pyruvate carboxylase (PC) activities. Those fed neither supplemental biotin nor fat and those fed both showed equal liver ACC activity.